Introduction:
Respiration commonly known as the inhalation and exhaling or breathing has a more little known definition. This is the definition that involves the cellular level of eukaryotic cells. Cellular respiration may best be described by the following equation: C6h1206+602-6CO2+6H20+36ATP. ATP that is the energy needed for a cell to function as part of cellular respiration. ATP is needed to power the cell processes. If cells are denied energy they will die. The second law of thermal dynamics says energy is lost in the form of heat whenever energy changes form. ATP is stored. Glucose produced C02, water and ATP. Respiration may be said to be a controlled breakdown of glucose that produces ATP for cell activities to be carried out.
The purpose of the lab was to show the effect of temperature on the rate of respiration. Temperature increases the rate of reaction between molecules this will have a profound impact on the rate of respiration.
Rate is determined on how fast something is being consumed in a reaction, or how fast something is being crated. The rate of respiration is measured the easiest by the amount of C02 produced by the reaction or by the amount of 02 consumed.
The best way to measure gasses is by creating a closed system for an experiment to take place. Consuming oxygen would decrease the volume of gas while the production of CO2 will increase the production of gas. By using this principle the measurement of an organisms volume if it absorbs CO2 released in respiration can be attributed to the consumption of oxygen.
Hypothesis: If the temperature increases, then the respiration rate will also increase. The respiration rate will increase because more activity is going on. Experiment:
The experiment a simple respirometer will be used in this experiment to detect changes in gas volume. Changes of volume will be measured at five different temperature levels and compared. A chamber will be constructed using respiring material (peas) and a carbon dioxide absorbing agent. Gas volumes may be influence by outside factors like air and pressure and temperature. A second chamber used as a control will be used to measure any changes due to air pressure or temp that are out of a controlled experiments control.
The Procedure:
I prepared two large test tubes each should have an inch of KOH pellets on the bottom of the tube. Next a cotton ball is placed in each of the two test tubes
5. A second test tube was then filled with water and placed in a test
gars. These are then split into two three-carbon sugar phosphates and then these are split into two pyruvate molecules. This results in four molecules of ATP being released. Therefore this process of respiration in cells makes more energy available for the cell to use by providing an initial two molecules of ATP.
For this I used the scale provided by the program. The manometer is a U-shaped tube that held liquid. This aided the experiment by showing me how much oxygen was consumed by the rats while they were placed in the jar. The airtight, glass animal chamber was the equipment I used to have an isolated area for the rats to go while I monitored their breathing. The tubes that connected this to the manometer had clamps that allowed me to block the passage way of air into the glass chamber or to stop air flow into the manometer while I collected the results.
For this experiment we have prepared to water baths. One bath is ice water, around 10 degrees Celsius, the other warm, around 25 degrees Celsius. Both water bath...
Equipment = == == == == § Hydrochloric Acid § Gelatine § Tile § Test tube § Rubber Bung § Scalpel § Ruler § Stop Clock § Goggles § Measuring Cylinder Experiment =
6. Place the test tube in the beaker. Secure the test tube and thermometer to the retort stand using clamps. Begin heating the water bath gently.
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
Cellular respiration is the process of converting glucose and oxygen into carbon dioxide and water while producing energy in the form of ATP. This process takes place throughout the mitochondria. First, glycolysis occurs in the cytosol of the cell; glucose is broken down into two pyruvates and produces NADH and some ATP. Pyruvate is then broken down into acetyl CoA and carbon dioxide is released as a byproduct. In the matrix, Krebs Cycle takes place, and acetyl CoA is broken down into NADH and FADH2. In between the matrix and intermembrane space, oxidative phosphorylation occurs; NADH and FADH2 give off protons which are pumped out of the Electron Transport Chain. NADH and FADH2 are converted into NAD+ and FAD, and they are ready to accept
Before conducting my experiment I will research into, amongst other things, the factors that affect the rate of a reaction. This is so that I may enough information to understand the effect of temperature on the rate of a reaction and also gain appropriate understanding to make a suitable prediction as to what the outcome of my experiment will be.
Under normal conditions, CO2 production through cellular respiration, and excretion through ventilation are balanced and
Cellular respiration uses oxygen and sugar to make carbon dioxide, water, and 36 ATP. The process of cellular respiration is glycolysis, then next kreb's cycle then, electron transport chain. The electron transport chain equals 32 ATP, the Krebs cycle equals 2 ATP and glycolysis equals 2 ATP also, which in the end, pulling all 3 stages together equals 36 ATP per glucose. Cellular respiration is a set of metabolic reactions and processes that take place in the cells of organisms to convert biochemical energy from nutrients into ATP, and releases waste products. Cellular respiration is the foundation for energy in a cell.
tube. Add 6 mL of 0.1M HCl to the first test tube, then 0.1M KMnO4 and
Independent variables: The temperature of hcl gas will be decreased and increased throughout the experiment.
According to our text, Campbell Essential Biology with Physiology, 2010, pg. 78. 94. Cellular respiration is stated as “The aerobic harvesting of energy from food molecules; the energy-releasing chemical breakdown of food molecules, such as glucose, and the storage of potential energy in a form that cells can use to perform work; involves glycolysis, the citric acid cycle, the electron transport chain, and chemiosmosis”.