Plant Chromatography Essay

In this experiment we are using a process called “chromatography” in order to accurately identify and separate the photosynthetic pigments from one another. A small sample of spinach solution is placed onto chromatography paper and made contact with a solvent. The result of solvent as it moves through the paper due to capillary action are observed and recorded. The results show that both the sample and the solvent move along the chromatography paper at the same rate. As the sample moves through the paper, the sample begins to separate itself into different pigments. Components of the mixture which contain a strong attraction to the paper with reside closer to the bottom of the chromatography paper. Where as the components which contain a weaker

Photosynthesis is a very complex system in which light energy is broken down and converted into usable chemical energy for the plant. For example, light energy (usually from the sun), carbon dioxide (usually from the exhale of mammals), and water are used by a plant to make chlorophyll and help the plant grow. In return the plant releases oxygen and produces sugar. This process repeats itself over and over again.

Through the process of photosynthesis, a plant leaf is the part of the plant that sucks in the oxygen and carbon dioxide. When this happens, the leaf, then makes a substance called chlorophyll. Chloraphyll. Inside of the plant leaf, sunlight and water enters and a light reaction occurs. From there ATP is given off and the energy is moved to the Calvin cycle. In the Calvin cycle carbon enters in the form of Carbon Dioxide and leaves in the form of glucose. A plant can produce pigments which is defined as a chemical substance that reflect only certain light wavelengths and absorb certain other wavelengths of

A line was drawn approximently one centimeter from the triangle. This line represented the “start line” for the experiment. Using the mortar and pestle, a large piece of spinach and 5 ml of 90% isopropyl alcohol was grinded until a thick liquid was created. Chromatography solvent (90% Isopropyl Alcohol) was then placed into a Microcentrifuge tube and eight drops was transferd onto the line of the chromatography paper using the capillary tube. One Centimeter of the chromatography solvent was added to the chromatography jar and the chromatography paper was placed in the jar so the tip of the triangle dipped into the solvent. After 15 minuets the chromatography paper was observed and the results were recorded. When the solvent line reached approximately one centimeter from the top of the chromatography paper, the paper was removed and observations of the furthest point of the solvents progress was recorded before the line