Chromatography

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Recommended: Note on chromatography

Chromatography is a method of separating and analyzing complicated substances. This is done in two phases, a mobile phase and a stationary phase .During the stationary phase, said substance is stationary, while during the mobile phase, the substance moves in a specific direction. During the mobile phase, the substance is filtered through the stationary phase. The stationary phase in necessary in order for the substances to be separated even though it doesn?t involve movement of the substance because it filters the substance through the stationary phase.. Since the substance is made of different, specific substances, each can go though the process of chromatography at different rates. This causes the components of the substance to be moved over materials made for absorption at different times. This makes the different components of the substance absorb at different rates. This is done numerous times and is a very precise method of separation. This process can be used to separate a wide variety of things, and can be used to separate most volatile or soluble substances. This process is used many like because it is gentle enough to separate delicate solutions, like those of proteins.

There are many types of chromatography, the types of which are as follows: Gas Chromatography, Liquid Chromatography, Ion Exchange Chromatography, and Affinity Chromatography . Gas Chromatography uses a pressurized gas camber to filter gasses by either thermal conductivity or flame ionization. There are three types of gas chromatography: capillary gas chromatography, gas adsorption chromatography and gas-liquid chromatography. Capillary gas chromatography used more often than any other type of gas chromatography. In this form of chromatography...

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...ntro to Chromatography." 25 Feb. 2008

Environ/CHROMO/chromintro.html>

Carrier, Rebecca, and Julia Bordanaro. "Gas Chromatology." 25 Feb. 2008

Carrier, Rebecca, and Julia Bordonaro. "Liquid Chromatography." 25 Feb. 2008

Carrier, Rebecca, and Julia Bordanaro. "Ion Exchange Chromatography." 25 Feb. 2008

Carrier, Rececca, and Julia Bordonaro. "Affinity Chromatography." 25 Feb. 2008 < http://www.rpi.edu/dept/chem-eng/Biotech- Environ/CHROMO/chromaffinity.html>

"Chromatography." Shaffald Hallam University. 25 Feb. 2008

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