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The effects of concentration on osmosis
Determining the effect of glucose concentration on osmosis
About Osmosis
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Recommended: The effects of concentration on osmosis
Hypothesis:
The concentration of a solute does affect the rate of osmosis over time, the higher the concentration of a solute, the faster the rate of osmosis. This happens because, in a semi-permeable membrane the water is the only through that can move through. In this case our solute is sugar, and sugar cannot fit through the pores in the membrane. When there is more sugar the difference in concentration is greater. That difference makes all the molecules want to go. That results in osmotic pressure being built up. Everything moves, the sugar tries to get through, but the pores don’t accommodate it. It being neat the pores block the water going through. That means that the water can only go through less pores because more sugar is blocking
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We take a long strand of dialysis tubing, and split it into nine 3 inch pieces.
2. Fill a flask with pure water (0% sugar), one with 0.2M water, another with 0.4 M water, and the last one with 0.8M water.
3. Also fill a smaller flask with pure water as well.
4. Take a piece of dialysis, find the mass of the dialysis, and the zero out the scale on which the dialysis was measured.
5. Then take the tubing, and dip it into the water to open it up, do it on both sides.
6. Next tie one side tight, and fill the tubing with water, and once it is filled to the top, close the top and tie it as well.
7. Then measure the mass of sphere, the drop it in the 0.2 solution for 7 minutes.
8. After time is up take the mass of it again.
9. Then record the changed mass.
10. Repeat 3 times for both 0.2M and 0.8M.
11. Then make the same ball for the 0.4 solution except put tape around
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After extensive review, we realized that this was not the case. We had conducted three different tests. In one test we had dropped our dialysis tube into a flask where the concentration was 0.8M and in that trial, we got an average of 0.1566g of water lost(three trials) . With the trials of 0.2M, the info shows that 0.27g of water were lost on average. The last part of the experiment we conducted a trial with the tubing in 0.4 water, but the catch was that we had surrounded the tubing with tape. That tape was to simulate the 0.8M solution because we felt that with the 0.8M solution, there was more sugar that blocked pores on the semi-permeable membrane. We believed that since the sugar block some of the pores, it would be like covering part of the faucet spout and it would make the water move through at a stronger and faster rate. After the trials, our prediction was correct in a sense; the tape did affect the osmosis. The tape had affected the osmosis, but it had had less mass lost than we expected. We conducted this trial before we had done the 0.8M solution, so this was against our beliefs at that stage. The data showed that the tape had made the dialysis tube lose 0.1866g on average. Our initial hypothesis stated that in the higher concentration, the sugar would be in an abundance and it would cover up the pores. We had thought that the pressure built up by that was
Start with the hot water and first measure the temperature. Record it. 8. Then pour 40 ml into the beaker. You can measure how much water was used by looking at the meniscus.
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
6. Place the test tube in the beaker. Secure the test tube and thermometer to the retort stand using clamps. Begin heating the water bath gently.
The potato tubes in higher concentration sugar solution will shrink
* In the first dialysis tube (bag A), we would insert ten mL of one
So what is happening is water is moving from a high concentration, i.e. a dilute solution to a more concentrated solution. Preliminary Work on Osmosis I am going to test carrots in a small osmosis experiment to see how the experiment works with carrots and salt solution and water. Variables 1) Temperature - Keep the same 2) Number of days left soaking - Keep the same 3) Size of beaker - Keep the same 4) Size of carrots - Keep the same 5) Concentration of salt solution -
In osmosis, water can travel in three different ways. If the molecules outside the cell are lower than the concentration in the cytosol, the solution is said to be hypotonic to the cytosol, in this process, water diffuses into the cell until equilibrium is established. If the molecules outside the cell are higher than the concentration in the cytosol, the solution is said to be hypertonic to the cytosol, in this process, water diffuses out of the cell until equilibrium exists. If the molecules outside and inside the cell are equal, the solution is said to be isotonic to the cytosol, in this process, water diffuses into and out of the cell at equal rates, causing no net movement of water. In osmosis the cell is selectively permeable, meaning that it only allows certain substances to be transferred into and out of the cell.
I am going to carry out an experiment to measure the change in mass of
Investigate the Osmosis of Potato Cells in Various Salt Solutions. Introduction I have been asked to investigate the effect of changing the concentration of a solution on the movement of water into and out of potato cells. I will be able to change the input of my experiment. The input variable is the concentration of the solution.
According to osmosis theory as the concentration of the sucrose solution increases the particles water potential increases and becomes higher than the particles that are in the
Then the value was divided by 2 to give the radius -the length of the string was measured -two metal block were clamped with the string in between, the string was tied to the bob -the bob was pulled to the side and released -the time was started when the bob passed the reference line. -a full oscillation is when the bob passes the reference line forth back and forth again. - the length of the string is increased or decreased in a regular pattern - the time was monitored by reading through the stopwatch. -the same brass ball was used over the experiment to keep the mass constant. - the length was varied 10 times with a 10 cm difference.
confirm this by weighing the ball on the scale used in step one. 4. Cover the whole by placing one layer of duck tape over it and ensuring that no sand comes out of it. 5. repeat step 2, at step 3 add.2 pounds and proceed to step 4 6. repeat step 2, at step 3 add.3 pounds and proceed to step 4 7.
In the diagram below on the right, we see two solutions divided by a partially/selectively permeable membrane (i.e. one that is porous, but allows water molecules through faster than dissolved substances). Text Box: Originally, the two solutions were; pure water, on the left, and sugar solution with a high sugar concentration, on the right. The pure water solution is said to have higher water potential than the concentrated sugar solution, because the water will flow from the area of high concentration of free water molecules (the dilute solution), to the area of low concentration of free water molecules (the concentrated solution). In other words, to the sugar solution.
The higher the levels of concentration were, the more significant the weight gain was. This meaning that high concentration creates more pressure resulting in weight gain. This pressure is created due to the attempt to diffuse rapidly to a lower concentration. The higher the concentration the bigger the urge to equalize. Table 2 shows the raw data from the experiment which allowed us to track that higher concentration effects osmosis.
the proper way to count a fluid on a hemacytometer. Recently our lab purchased two new