Lab Experiment: The Effectiveness of Different Antibiotics on Bacteria

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Objectives:

1. To investigate the effect of different antibiotics on bacteria

2. To develop problem solving and experimental skills, for example, information is accurately processed and presented, experimental procedures are planned, designed and evaluated properly, producing valid results, recording results, and valid conclusion is drawn.

3. To develop the aseptic techniques for preparing agar plates and bacterial culture.

Problem statement

Do antibiotics have the same effect on killing different types of bacteria?

Abstract:

The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment includes: the using of aseptic techniques for the preparation of agar plates. Paper discs containing different types of antibiotics then placed on the hardened agar plates. The inhibition zones of the bacteria on agar plates then is observed after 24 hours to determine the effectiveness of different types of antibiotics. The main result of this experiment is that the paper dics soaked with tetracycline has the greatest zone of inhibition in both Bacillus subtilis and E. Coli agar plates. Therefore, from this experiment, we can conclude that Tetracycline is the most effective antibiotics for Bacillus subtilis and E. Coli.

Introduction:

Antibiotics

Antibiotics are among the frequently prescribed medicine for treating bacterial infection. Finishing antibiotics is important to prevent remaining bacteria from developing resistance to the antibiotics. However, antibiotics are useless to treat diseases causing by viruses such as colds, flu, coughs, and bronchitis. (emedicinehealth, 2010). So...

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...om: http://www.emedicinehealth.com/antibiotics/article_em.htm Accessed on 6th February 2010

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Wikipedia. 2010. Aseptic technique. Available from: http://en.wikipedia.org/wiki/Aseptic_technique Accessed on 6th February 2010

Wikipedia. 2010. Bacillus subtilis. Available from: http://en.wikipedia.org/wiki/Bacillus_subtilis Accessed from 6th February 2010

Wikipedia. 2010. Escherichia coli. Available from: http://en.wikipedia.org/wiki/Escherichia_coli Accessed on 6th February 2010

Wikipedia. 2010. Micropipette. Available from: http://en.wikipedia.org/wiki/Micropipette Accessed on 6th February 2010

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