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Enzymes in everyday life
The effects of enzyme concentration on its activity
Investigating enzyme action
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Investigating the Effects Catalyse Has on Hydrogen Peroxide Solution
Aim: I am going to investigate the effects which catalyse has on a
hydrogen peroxide solution
Preliminary work
We would use potato to do the experiment because we had done a
previous experiment when we put a piece of potato and liver into a
hydrogen peroxide solution and the rate of reaction for the liver was
too fast to measure and time. But the potato had a slower reaction
which will be easier to time and we can measure the size of the froth
using a ruler.
Theory
Enzymes are special shaped protein molecules which act as catalyst and
work in living organisms. Enzymes help speed up the rate of reactions.
Each enzyme has a unique 3D shape which means that they work with
certain types of substrates. This is also known as ‘lock and key’
because the shape of the substrate fits into the shape of the enzyme,
just the way a lock and key do. Enzymes work by attaching itself to
the substrate molecule. The substrate molecule brakes down where the
active site is. The substrate molecule splits and the enzyme is left
unaffected. The factors that affect enzymes are: temperature, pH and
surface area. Enzymes are used in everyday life because all living
things produce enzymes. Saliva in a human produces a lot of enzymes
for digestion. The optimum temperature for these enzymes to work in is
37.5 °C. Enzymes also perform best at a pH which is around neutral.
When an enzyme reaches 40°C it becomes denatured. Enzymes are also
used in industry e.g. bakery, cheese making, starch processing and
production of fruit juices and other drinks
[IMAGE]
The substrate splits
.
Active site
[IMAGE]
[IMAGE]
This is a graph to show the rate of reaction of enzymes due to
temperature
graph showing effect of temperature
[IMAGE]
Prediction
I predict that the potato cylinder with the diameter of 10mm will
· I predict that the enzyme will work at its best at 37c because that
First of all we decided to use a plastic cup because it was easy to
Effect of Substrate Concentration on Catalase. Aims This is an experiment to examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme. Catalase. Background Information Enzymes such as catalase are protein molecules, which are found in.
The Effect of a Catalase on the Breakdown of Hydrogen Peroxide Aim To follow the progress of a catalysed reaction by measuring the volume of gas produced as the reaction proceeds. Using the initial rates of a series of experiments I will be able to find the orders of the reaction with respect to enzyme and substrate. Also to find out if concentration has an effect on the reaction when an enzyme is used to accelerate the breakdown of hydrogen peroxide.
Place one egg in a beaker. Fill this beaker with distilled water to just cover the egg. See Figure 1. Note the appearance of the water at this time and record your observation in Data Table 3. CAUTION: Be careful to avoid breaking glassware.
Investigating the Rates of Reaction Between Hydrogen Peroxide and Manganese Dioxide Aim: To be able to My aim for this investigation is to find out how concentration affects the rate of reaction between hydrogen peroxide and manganese dioxide. Equipment List -. Syringe filled with water (gas collector). Conical flask -.. Manganese dioxide powder -. Hydrogen Peroxide liquid -..
Analysis of the Decomposition Rate of Hydrogen Peroxide With Catalase As a Catalyst Aim: To measure the rate of decomposition of Hydrogen Peroxide with Catalase from a Yeast solution using PH as a variable. Hypothesis: The enzyme Catalase speeds up the Hydrogen Peroxide decomposition as its active sites match the shape of the Hydrogen Peroxide molecule. This process will only work at certain PH levels as the Enzyme sites may become disfigured at extremes. Logic suggests that Catalase will work well at PH7 Neutral, but due to the nature of Catalase removing Hydrogen Peroxide from human body cells a slightly acidic solution might work just as well. [IMAGE] This is based on the Key and Lock principle of the enzyme; [IMAGE] When various different PH values are present the shape of the Lock of the Enzyme varies, this can cause a slower rate of reaction, or in the event of the lock become completely deformed no reaction.
water and the slower the reaction will be. If the pot is close to the
Ø Then pour the catalyst into a test tube along with the H O and
How Hydrogen Peroxide Affects the Rate of Reaction of the Enzyme Catalase Introduction: Catalase, like all enzymes, is made up of protein molecules. It can be found in the cytoplasm of living tissue. It speeds up the decomposition of Hydrogen Peroxide, a metabolic waste product, into water and oxygen that can safely be removed from the cell. The type of reaction involved is known as a catabolic reaction (i.e. substrate broken down.)This is simply because the substrate enters the active site and is broken down, and leaves as 2 separate products, in this case water and oxygen: 2H2O2> 2H20 + O2
Investigating the Effect of Concentration on the Temperature Rise, Heat Evolved and Heat of Neutralization for the Reaction Between HCl and NaOH
I started with a paper towel, an 18 by 18-millimeter bowl and a rubber band. I also had twelve 88.7-milliliter dixie cups filled with 50 milliliters of frozen, red colored water. The first step in this experiment was ...
Investigation into the Factors that Affect the Enthalpy Change During a Displacement Reaction The Task -------- To investigate the factors affecting the enthalpy change during a displacement reaction. Background Knowledge =
Hydrogen Peroxide Investigation When hydrogen peroxide is added to a piece of fresh potato, bubbles of. gas are seen to form. When a bigger piece of potato is used, a larger. number of bubbles produced. A possible explanation for this.
An Investigation into the Decomposition of Hydrogen Peroxide Aim: To investigate the rate of decomposition of H2O2 with different amounts of catalyst (MnO2). Hypothesis: When H2O2 and a catalyst are mixed together, the catalyst would break down H2O2 into water and oxygen. This will result in bubbles being produced. With the data of these oxygen bubbles, the rate at which H2O2 decomposed could be found out. 2H2O2 (l) à2H2O + O2 The controlwould be to maintain the same temperature (room temperature) and to use the same amount of hydrogen peroxide (10ml) in all the tubes.