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How osmosis can be investigated
Osmosis investigation
How osmosis can be investigated
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Investigating Osmosis in Potatoes
Preliminary Experiment
Aim
The aim of this experiment is to determine the solute concentration of
potato tissue; i.e. To investigate at which point the sucrose
concentration is the same as the solute concentration inside the
potato (an equilibrium is reached)
Introduction
Osmosis is the movement of water molecules from an area of high
concentration; (a weak solution), to an area of low concentration; (a
strong solution), through a partially permeable membrane. My
experiment will prove that the concentration inside the potato is
lower than the concentration of the water molecules, outside the
potato if using distilled water or very low concentrations of sucrose
solution.
Preliminary Method
Cut two potato cylinders to the correct sizes e.g. 5cm in length, and
dry them out using paper towels. It is important to dry out the potato
cylinders because the excess water inside would have an effect on the
results, and this would therefore create an unfair experiment. Then
one must record their masses and lengths. Place them into the two
different solutions; 1.0M of sucrose solution and distilled water, and
leave them for fifteen minutes. One must leave the potato cylinders in
the two different solutions for at least fifteen minutes because this
is a minimum time, in which the starch particles will be able to
diffuse into the surrounding liquid, if indeed that is the outcome.
Then remove the potato cylinders and dry them out for a second time to
rid any excess water which is just clinging to the outside of the
potato cylinder, so that the only mass counted, is that of the liquid
which is inside the potato. Record their masses and lengths again. For
safety reasons I will be very careful when using the scalpel and
potato corer.
Equipment for my preliminary experiment
· One boiling tube with 30cm cubed of distilled water
· One boiling tube with 30cm cubed of 1.0M of sucrose solution
· Two potato cylinders, approximately the same mass and length
Conclusion In my conclusion, the potatoes with the lowest concentration gained the most mass, and would become hard relating back to the Turgor theory I stated earlier. In contrast to this, the potatoes in the most concentrated solution lost the most weight thus becoming plasmolysed and limp also relating back to the background I have mentioned earlier. Evaluation In general the experiment was succesful the results were consistent and also were in accordance with the theories made at the start.. The experiment could have been improved by: · More subjects used instead of potatoes · More potatoes · Wider time ranger · Different molarities Using this variety of methods could have improved the experiemnt, however I was generally satisfied with the results of this osmosis experiement.
We then cut our potato tubes with the cork borer and cut them with the scalpel so they were the same length and weighed them. We then put one potato tube in each test tube and then added the same amount sugar solution in to each tube. The concentration of sugar solutions varied in each test tube.
Osmosis in Carrots Background Osmosis is the diffusion of water from a dilute solution to a more concentrated solution through a partially permeable membrane, which allows the pass of water molecules but not solute molecules. [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE]If a cell is placed in a less concentrated solution water enters because the less concentrated solution will have a high concentration of water than the inside of the cell. Once the cell takes in maximum water the cell becomes turgid. If the cell was to be placed in a high concentrated solution, water would leave the cell because the cell would contain a low concentrated solution. So in the low concentrated solution there will be a high concentration of water and in the high concentrated solution there will be a low concentration of water.
* Note the mass down in the table at the end of the first page.
When I am not using them I will place them away from my experiment and
I am going to carry out an experiment to measure the change in mass of
So the experiment will be based upon the movement of water. The first potato chip will be placed in a zero percent solution. I believe that this will increase the mass. I believe this because the zero percent solution has a lower concentration than the cell sap inside the potato. The potato takes on the water through osmosis and the cell pushes out from inside the cell making it swell and become more rigid.
The Effect of Water Concentration on the Mass of Potato Tissue Aim: The aim of this investigation is to discover the effect of water concentration on the mass of potato tissue, and also to investigate the movement of osmosis through potato tissue. Scientific Knowledge: When a substance such as a sugar dissolves in water, the sugar molecules attract some of the water molecules and stop them moving freely. This, in effect, reduces the concentration of water molecules.
To investigate the osmotic effect of changing the concentration of sucrose solution; distilled water, 20% sucrose solution, 40% sucrose solution, 60% sucrose solution on the change in mass of potato cylinder after 30 minutes of being in solution.
This is because the water molecules passed from a high concentration in the water, to a low concentration, in the piece of potato. Therefore, the potato in higher water concentrations will have a larger mass than in higher sucrose concentrations. i.e. The potato pieces are shown to have water potential. The attached graph shows the concentration of the sucrose solution plotted against the average mean change in mass.
Investigating the Effect of Sugar Solution on the Weight and Size of Potato Cells Aim: To investigate whether the different concentration of sugar solution will affect the weight and size of the potato cells. General background information: Osmosis is defined as the movement of water or any other solution's molecules from an area in which they are highly concentrated to a region in which they are less concentrated. This movement must take place across a partially permeable membrane such as a cell wall, which lets smaller molecules (E.g. water) through but does not allow larger solute molecules to pass through. The molecules will continue to diffuse until the area in which the molecules are found to reach a state of equilibrium, meaning that the molecules are equally distributed throughout the cell, with no area having a higher or lower concentration than any other hence equal. Hypothesis/prediction: For this particular investigation I believe that the lower the concentration of the sugar solution in the test tube the mass of the potato will be greater and the longer the potato cells will be.
water in the potato, then the water will go out of the potato and into
Prediction I think that when the potato is placed in distilled water the potato mass will increase. This is because water, has, if pure, a weaker concentration than the potato, and the water molecules move in through a partially permeable membrane by osmosis. If the potato is in a high concentration salt solution, the potato mass will decrease because the potato is less concentrated than the salt solution, and the water will move through the partially permeable membrane into the stronger solution. However, if the potato mass stays the same after the experiment, this means that the water/salt solution and the potato must be of equal concentration.
The Effect of Solute Concentration on the Rate of Osmosis Aim: To test and observe how the concentration gradient between a potato and water & sugar solution will affect the rate of osmosis. Introduction: Osmosis is defined as, diffusion, or net movement, of free water molecules from high to low concentration through a semi-permeable membrane. When a substance, such as sugar (which we will be using in the experiment we are about to analyse), dissolves in water, it attracts free water molecules to itself, and in doing so, stops them from moving freely. The effect of this, is that the concentration of (free) water molecules in that environment goes down. There are less free water molecules, and therefore less water molecules to pass across a semi-permeable membrane, through which sugar molecules and other molecules attached to them are too big to diffuse across with ease.
Determining the Concentration of the Cell Sap in Potato Storage Tissue Aim: To determine the concentration of the cell sap in potato storage tissue. By using Osmosis, determine what the sugar concentration of cell sap is. Prediction I predict that the potato segment in the distilled water will definitely gain in weight because the solution outside it has a much higher concentration of water then in the cell sap meaning Osmosis will occur and the potato segment take in water. I predict that 0.2M sugar solution will also gain weight because it still has quite a high concentration of water outside the potato. The potato in the 0.4M solution will gain weight but the potato in the 0.6M and 0.8M solutions will lose weight.