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Importance of seed preservation
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INTRODUCTION
Seed proteins can be broadly classified into two categories: housekeeping proteins and storage proteins. Seed storage proteins are group of proteins synthesized mainly during seed development, serve as a major nitrogen reserve which are utilized during germination of the seed. They are the source of necessary free amino acids and nitrogen to the growing plant with the sole purpose of providing proteins (nitrogen and sulphur source) required during germination(Chua et al., 2008). These are a complex mixture of different proteins which differ in structure, size, shape, amino acid composition, solubility and other physiological, functional, nutritional, nutraceutical and nutragenomic properties. Storage proteins are generaly found
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S., & Mandal, 2000; Bojórquez-Velázquez et al., 2016). Because of the abundance of these storage proteins, they are mainly responsible for the nutritional quality of the human diet. Apart from nutritional qualities, seed proteins gain special importance due to their ability to influence the functional quality, which inturn determines the end use of grain in food processing (Bojórquez-Velázquez et al., 2016). Seed storage proteins accumulate in the cotyledon and embryo of dicotyledonous plants and in the endosperm of monocotyledonous plants. Salt-soluble globulins are the predominant storage protein in legumes and are grouped under two classes, 7S and 11S globulins, while alcohol-soluble prolamins are the major storage proteins in cereals. Oats and rice are exceptional, in which the major storage proteins are …show more content…
Oilseeds occupy very important position in the Indian agricultural economy. The varieties of oilseeds grown in India are ground nut, soybean, sesame, sunflower, cotton, safflower, mustard, etc. Oilseed storage proteins are generally rich source of nitrogen. In oilseeds, large amount of proteins are present in protein bodies (Pernollet and Mosse, 1982). Utilization of protein from unconventional sources of vegetable origin, including oilseeds for edible purposes has been explored. The oilseed proteins are categorized into 2 group’s namely low and high molecular weight fractions and based on the sedimentation coefficient the protein fractions are designated as 11S, 7S, 2S protein (Prakash and Rao,
n.d. - n.d. Peptides and Proteins. Proteins. Retrieved July 25, 2008, from http://www.cd http://www.cem.msu.edu/reusch/VirtualText/protein2.htm Ophardt, C. E. (2003).
Introduction: Gibberellin is a plant hormone that is involved in the production of amylase which is vital for the conversion of starch into maltose into glucose molecules required for the embryo to grow. It does this by “regulating the genes that are involved in the synthesis of amylase. In barley seeds, it has been shown that the application of gibberellin causes an increase in the transcription of mRNA coding for amylase”. 1(Jones, p.120)
The Structure and Function of Carbohydrates Large biological molecules are called macromolecules, there are giant molecules (polymers) made up of repeating units (monomers). Carbohydrates are one of the main classes of biological molecules. Macromolecule units (monomers) are joined together by condensation reactions and hydrolysis reactions split macromolecules down into their individual units. Carbohydrates are molecules that contain elements of carbon, hydrogen, and oxygen. Carbohydrates have a 2:1 hydrogen to oxygen ratio, there are twice as many hydrogen atoms as oxygen atoms (the same proportion as in water).
Abstract/Summary: “Proteins account for more than 50% of the dry weight of most cells, and they are instrumental in almost everything organisms do” (Campbell, 1999). The significance of proteins to the continuation of our biological systems is undeniable, and a study of how to quantify proteins seems an appropriate introduction to our studies of biology. In order to study proteins we must first know how to separate then quantify the amount using basic principles of experimental design such as a standard curve. In this experiment we wish to quantify the amount of previously extracted protein by measuring the absorbance of the unknown amount and determining its concentration by overlaying it against a standard curve of the absorbance of known concentrations of the protein. We used the dye agent Bradford Protein Assay to get an absorbance of 0.078, 0.143, 0.393, 0.473, and 0.527 at the protein’s respective concentrations of 0.28, 0.56, 0.84, 1.12, and 1.40 mg/mL. When a best-fit line was applied to the standard curve, and the absorbance of our unknown concentration (0.317 A) plotted, we estimated a concentration of around 0.84 mg/mL of protein. Our calculations indicated a quantity of 168 mg of protein, which was an approximately 8.96% yield of the projected 1875 mg that was expected. Errors that may have led to this small yield percentage may have stemmed from our previous lab and our initial attempts to extract the desired amount of protein.
Proteogenomics is a kind of science field that includes proteomics and genomics. Proteomic consists of protein sequence information and genomic consists of genome sequence information. It is used to annotate whole genome and protein coding genes. Proteomic data provides genome analysis by showing genome annotation and using of peptides that is gained from expressed proteins and it can be used to correct coding regions.Identities of protein coding regions in terms of function and sequence is more important than nucleotide sequences because protein coding genes have more function in a cell than other nucleotide sequences. Genome annotation process includes all experimental and computational stages.These stages can be identification of a gene ,function and structure of a gene and coding region locations.To carry out these processes, ab initio gene prediction methods can be used to predict exon and splice sites. Annotation of protein coding genes is very time consuming process ,therefore gene prediction methods are used for genome annotations. Some web site programs provides these genome annotations such as NCBI and Ensembl. These tools shows sequenced genomes and gives more accurate gene annotations. However, these tools may not explain the presence of a protein. Main idea of proteogenomic methods is to identify peptides in samples by using these tools and also with the help of mass spectrometry.Mass spectrometry searches translation of genome sequences rather than protein database searching. This method also annotate protein protein interactions.MS/MS data searching against translation of genome can determine and identify peptide sequences.Thus genome data can be understood by using genomic and transcriptomic information with this proteogenomic methods and tools. Many of proteomic information can be achieved by gene prediction algorithms, cDNA sequences and comparative genomics. Large proteomic datasets can be gained by peptide mass spectrophotometry for proteogenomics because it uses proteomic data to annotate genome. If there is genome sequence data for an organism or closely related genomes are present,proteogenomic tools can be used. Gained proteogenomic data provides comparing of these data between many related species and shows homology relationships among many species proteins to make annotations with high accuracy.From these studies, proteogenomic data demonstrates frame shifts regions, gene start sites and exon and intron boundaries , alternative splicing sites and its detection , proteolytic sites that is found in proteins, prediction of genes and post translational modification sites for protein.
Gliadins’ are mostly monomeric proteins with molecular weights between 28,000 and 55,000 and can be classified depending upon their different primary structures into the a/b-, y- and o-type. They can be found in wheat and in different other cereals within the grass genus Triticum (Wieser, 1996). For each type, the structural differences between them are small. This is because of the substitution, deletion and insertion of single amino acid residues (Weiser, 2007). These proteins contain unusually high amounts of glutamine and proline and have large regions of repetitive sequences. As most Gliadins’ are monomeric proteins which contain eight conserved cysteine residues, some contain an extra cysteine residue which allows them to be linked with other gluten proteins to form large polymers which are essential for flour quality (Altenbach et al, 2010).
This research aims on the comparison of the brown and milled rice grain has been compared with that of the non-transgenic rice of the same variety in nutritional composition. In this study, the nutritional components, as well as the anti-nutrient levels, were measured. And it established that apart from the increased level of iron and zinc in transgenic seeds.
In this experiment, lipids from ground nutmeg are extracted using a combination of solvents and identify the lipids through chromatography. The purpose of using solvent combinations is to elute the lipids based on their polarity to binding of the silica gel. The chromatography is performed on a silica gel plate and the use of iodine to visualize the lipids. By calculating the Rf values for each compound and comparing them to the known lipids, we are able to distinguish the lipids within the grounded nutmeg.
Janick. J. (2011). Center for New Crops & Plant Products - Department of Horticulture and
For example, some key plant proteins are often low in methionine, tryptophan, lycine and isoleucine.
Protein synthesis is one of the most fundamental biological processes. To start off, a protein is made in a ribosome. There are many cellular mechanisms involved with protein synthesis. Before the process of protein synthesis can be described, a person must know what proteins are made out of. There are four basic levels of protein organization. The first is primary structure, followed by secondary structure, then tertiary structure, and the last level is quaternary structure. Once someone understands the makeup of a protein, they can then begin to learn how elements can combine and go from genes to protein. There are two main processes that occur during protein synthesis, or peptide formation. One is transcription and the other is translation. Although these biological processes slightly differ for eukaryotes and prokaryotes, they are the basic mechanisms for which proteins are formed in all living organisms.
In order for a seed to germinate, many conditions must be met. Firstly a seed must be viable. If a seed is viable it require a suitable environment with proper levels of moisture, suitable temperatures, oxygen, fertile soil, and proper pH levels to name a few. However, as many of us have experienced, some viable (living) seeds will NOT germinate, even when given the perfect conditions. In the field of botany, this phenomenon is known as “physiological seed dormancy” (will be referred to as PSD from here on out). In plain language, PSD is like a “hibernation” stage that occurs after seed maturation but before germination.
1443 - 1460. Keener, K., Hoban, T. and Balasubramanian, R. 2014. Biotechnology and its applications. [online] Available at: http://www.ces.ncsu.edu/depts/foodsci/ext/pubs/bioapp.html [Accessed: 11 Apr 2014].
Proteins (macronutrient), which are found in animal products, nuts and beans, they help to build new cells, maintain tissue and synthesis new proteins essential for performing basic bodily functions. Proteins are in abundance in the human body and are present in the outer and inner membranes of all living cells (Dummies, 2018). Proteins are essential for building new cells, maintaining tissue and helping new proteins needed for basic bodily function (
Plant nutrition is area of plant biology that is of the utmost importance for the proliferation of plants. Without proper nutrition, plants would simply cease to exist unless drastic alterations were made. There are certain elements that are required for the plant to grow and reproduce; these elements are known as essential elements. There are three requirements of an essential element: the element must be required for the completion of the plant’s life cycle, the element must not be replaceable by another element in whole, and finally the element must be direction involved in the metabolism of the plant. Chemical compounds that are involved in proper nutrition have been designated as nutrients, and further classified as macronutrients and micronutrients. Macronutrients are needed for growth, metabolism, and many other functions, but are designated as “macro” because they are required in larger amounts. Macronutrients include carbohydrates, proteins, and fat molecules. Micronutrients have a much wider function that depends on the exact micronutrient. Micronutrients are designated as so because they are needed in much smaller amounts when compared to macronutrients. Examples of micronutrients include vitamins and minerals.