Importance Of Pharmaceutical Analysis

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CHAPTER I INTRODUCTION Pharmaceutical analysis plays a vital role in quality assurance and also in quality control of bulk drugs and pharmaceutical formulations. It is a branch of analytical chemistry that includes separation, identification and determination of the relative amounts of components in the sample. The pharmaceutical analysis deals with the characterization of the sample qualitatively as well as quantitatively. Qualitative analysis reveals the chemical identity of the sample and quantitative analysis establish the respective amounts of components in the sample in numerical terms (Rappoport and Liebman, 2009; Paul et al., 2011). Fast increase in pharmaceutical industries and drug production in various parts of the world has leads …show more content…

Kuhn and A. Winterstein) published a paper (Ettre & Sakodynskii, 1993) on purification of xanthophylls on CaCO3 adsorption column following the process described by Tswett. In the year 1941, partition chromatography was discovered by R. L. M. Synge and A. J. P. Martin at Cambridge University in the UK, (Martin & Synge, 1941) for that in 1952 they were awarded the Noble Prize. In 1952, Martin and Synge published a seminal paper (Martin, 1941) which, along with the paper of A.T. James and A. J. P. Martin (Martin & Synge, 1952), laid a foundation for the quick growth of chromatographic techniques that shortly followed. Prior to the 1970's, few good chromatographic methods were commercially obtainable to the laboratory scientist. During 1970's, most chemical separations were performed using different techniques including open-column chromatography, TLC (thin-layer chromatography) and paper chromatography. However, these chromatographic techniques were insufficient for resolution between similar compounds and quantification of compounds. During this time, to decrease flow-through time pressure liquid chromatography began to be used, thus reducing time taken for purification and separation of compounds being isolated by column chromatography. As flow rates were Inconsistent, the question if it was good to have a constant flow rate or constant pressure was debated (Chatrabhuji et al., 2015). In the mid-1970's, High pressure liquid chromatography (HPLC) was developed and improved rapidly with the development of different column packing materials and the additional suitable detectors. Some new methods including reverse phase liquid chromatography allowed for better separation between very similar molecules. By 1980's, for the separation of chemical molecules, HPLC was widely used. New techniques enhance purification, separation,

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