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Amylase on the rate of starch digestion
Effects of amylase concentration on its activity
Effects of amylase concentration on its activity
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Recommended: Amylase on the rate of starch digestion
According to Authority Nutrition, Carb Cutters block 50-65% of carb-digesting enzymes.(“What Are Carb Blockers”). How accurate is this statistic? In a recent study I participated in, I studied whether Carb Cutter prevents amylase from digesting starch. Carb Cutter is used to block starch from digesting in the body. Normally, starch is broken down by the enzyme amylase into glucose. However, when Carb Cutter is used, it prevents the function of the enzyme amylase, causing starch not to be digested into glucose. Many times Carb Cutters are used to help with weight loss since it is claimed to stop starch from being digested. However, there may be some suspicion on whether or not Carb Cutters actually work or if they are just a fraud. My experiment studied whether or not Carb Cutters actually work and how effective they really are. …show more content…
To begin the study, I first calculated how much of each solution I would need. I knew that the final volume of my reaction solution needed to me 30ml, so I calculated how much of starch, amylase, and tris buffer I would need. I used the formula Concentration (initial stock solution) x Volume (initial stock solution)= Concentration (final solution) x Volume (final solution). Using this formula, I found that I would need an initial concentration of 21 ml of starch, 1 ml of amylase, and 8 ml of the tris buffer. After calculating the amounts of substances I would need, I created two different solutions, one with the Carb Cutter and one without. Carb Cutter claims to block starch, however, to find this I needed to test the absorbance level of the control to compare the effect Carb Cutter had on the solution. Below is a graph showing the concentration of the control reaction over one minute intervals through the
The unknown bacterium that was handed out by the professor labeled “E19” was an irregular and raised shaped bacteria with a smooth texture and it had a white creamy color. The slant growth pattern was filiform and there was a turbid growth in the broth. After all the tests were complete and the results were compared the unknown bacterium was defined as Shigella sonnei. The results that narrowed it down the most were the gram stain, the lactose fermentation test, the citrate utilization test and the indole test. The results for each of the tests performed are listed in Table 1.1 below.
Input variables In this experiment there are two main factors that can affect the rate of the reaction. These key factors can change the rate of the reaction by either increasing it or decreasing it. These were considered and controlled so that they did not disrupt the success of the experiment. Temperature-
These labels indicated the lactose solution that was be placed into the mini-microfuge tubes. The varying lactose ph solutions were obtained. The four miniature pipets were then used, (one per solution,) to add 1mL of the solution to the corresponding mini-microfuge tubes. When this step is completed there were two mini-microfuge tubes that matched the paper towel. Then, once all of the solutions contained their respective lactose solutions, 0.5mL of the lactase enzyme suspension was added to the first mini-microfuge tube labeled LPH4 on the paper towel, and 4 on the microfuge tube. As soon as the lactase enzyme suspension was added to the mini-microfuge tube, the timer was started in stopwatch mode (increasing.) When the timer reached 7 minutes and 30 seconds, the glucose test strip was dipped into the created solution in the mini-microfuge tube for 2 seconds (keep timer going, as the timer is also needed for the glucose strip. Once the two seconds had elapsed, the test strip was immediately removed, and the excess solution was wiped gently on the side of the mini-microfuge tube. The timer was continued for 30 addition seconds. Once the timer reached 7:32 (the extra two seconds accounting for the glucose dip), the test strip was then compared the glucose test strip color chart that is found on the side of the glucose test strip
The control for both curves was the beaker with 0% concentration of substrate, which produced no enzyme activity, as there were no substrate molecules for...
...eases, including temperature. It is determined from the data that the reaction is more likely to have a step wise mechanism than a concerted due to the small – ΔS and a relatively large value of ΔH from the tables. Due to some errors, it is best to perform another experiment for future protocols. In addition with the variance the 35°C where at one point the absorbance levels off and then increases. In comparison to the rate constant against temperatures, at 25°C it is higher than 35 and 45. More test is required to ensure proper determination of the rate constant at those temperatures.
Abstract: Gibberellic acid is a plant hormone that is used to stimulate growth and fasten the germination of plants. When Gibberellic acid used on plants, it produces bigger and fuller leaves following by elongating the stems. This experiment was designed to determine the effect that Gibberellic acid will have on the growth of a seed germination. As performed in class, three types of radish seed were treated with Gibberellic acid to see the effects the acid will have on those three seeds. During the treatment plan, the three seeds received a different amount of acid and water five times a week. For instance, Seed A got approximately 2.5ML of Gibberellin acid, seed B got 5.0ML, and seed C got 10ML, following by seed A getting 17.5ML of water, seed B getting 15.0ML, and seed C obtaining 10ML of water. However, based on this treatment plan, seed A and B showed no growth. While, the seed that consumed more acid, which was Seed C showed rapid growth. In this case, the only possible explanation for this surprising result could be that the Seeds
The independent variable for this experiment is the enzyme concentration, and the range chosen is from 1% to 5% with the measurements of 1, 2, 4, and 5%. The dependant variable to be measured is the absorbance of the absorbance of the solution within a colorimeter, Equipments: Iodine solution: used to test for present of starch - Amylase solution - 1% starch solution - 1 pipette - 3 syringes - 8 test tubes – Stop clock - Water bath at 37oc - Distilled water- colorimeter Method: = == ==
I blended on high to make the potatoes more liquid-like. I grabbed the cheesecloth and placed on the top of the blender. I poured the potato extract on the container and labeled it. I found out that I have to make 1% sugar solution so I grabbed the sugar and measured into 5 grams on the scale. I added 5 grams of sugar on 250 ml graduated cylinder and poured the water into the cylinder. I mixed the sugar with water and poured it into the saucepan. I refilled the water into the graduated cylinder and poured into the saucepan. I turned on the heat of the stove and saw the sugar dissolved. I poured into a container and labeled 1% sugar solution. I repeated the same thing with 1% salt solution by using 1 gram of salt and filled the water into graduated cylinder by 100 ml. I answered question three. In the first experiment, I grabbed four transfer pipets and used it to put solutions into the test tubes by 3ml. I labeled it and placed into the plastic cups so it can stand upright. I grabbed each test tube and poured 2 ml of catalase solution into it. I also tapped and swirled to measure the bubbles by using the ruler. I wrote the numbers into the lab report. In the second experiment, I labeled the room
Severely restricting carbohydrates is not healthy and offers little advantages in terms of fat loss. Consumers of low carbohydrate products are often deceived into believing all low carbohydrate products are better for their health. However, usually when a product claims to have a low amount of carbohydrates, it fails to mention the increase of fats and proteins the product gains to compensate for the lost carbohydrates. In reality low carbohydrate diets increase health risks and give dieters false hopes. Carbohydrates are significant in supplying energy to the body’s needs. Through plenty of carbohydrates, especially for active people and athletes, the body is able to use its’ adequate amounts of energy efficiently.
site) then the quicker the starch (substrate) will be broken. down, resulting in a faster reaction rate. Therefore, a smaller amount. of amylase will result in a slower reaction rate. [ IMAGE] Text Box: Fischerâ€TMs †Lock and Keyâ€TM hypothesis (1890).
Carb cycling, a diet plan that alters the intake of carbs on a daily basis in order to keep metabolism high without a plateau or muscle loss, is growing in popularity for everyday people, endurance athletes, power lifters, and body competitors. That is not too surprising considering its simplicity and effectiveness. The basis of carb cycling is that from day-to-day one’s diet is constantly changing in the amount of carbohydrates and fats that are consumed. This results in overall fat loss while gaining muscle with more psychological ease, making it one of the easiest diets out there; however, there is no day-by-day instruction manual to follow. It takes knowledge and knowing one’s body. Everyone’s body is different; thus, every diet will be different, but learning how to alter the diet based on one’s body and daily routines is the key. To be more specific, endurance athletes have very different nutrition needs than power lifters, non-endurance sports, and less active people.
Investigating the Effect of Enzyme Concentration on the Hydrolysis of Starch with Amylase Aim: Investigate the effect of enzyme concentration on the rate of an enzyme-controlled reaction. Using amylase and starch as my example. Introduction: I am investigating the effect of the concentration of the enzyme, amylase on the time taken for the enzyme to fully breakdown the substrate, starch to a sugar solution. The varied variable will be the concentration and all other variables are going to be fixed. The different concentrations will be: 0.5% 0.75% 1.0% 1.5% 2% An enzyme is a class of protein, which acts as a biological catalyst to speed up the rate of reaction with its substrates.
Volume of substrate used Continuous Yes Type of enzyme Discontinuous Yes Overall mass of piece of meat Continuous No Type of substrate used Discontinuous Yes Temperature of substrate Continuous Yes pH of substrate Continuous No -- Concentration of substrate used Continuous Yes The independent variable I have chosen, or the one to be changed throughout the experiment will be 'the concentration of substrate used', which will range from 0.25M to 1.25M with increments of 0.25M. With reference to the table above, it has been chosen, as it is continuous (i.e. it has a numerical value of some sort and this can be altered) so the results can facilitate a graph.
There were five test solutions used in this experiment, water being the control, which were mixed with a yeast solution to cause fermentation. A 1ml pipetman was used to measure 1 ml of each of the test solutions and placed them in separated test tubes. The 1 ml pipetman was then used to take 1ml of the yeast solution, and placed 1ml of yeast into the five test tubes all containing 1 ml of the test solutions. A 1ml graduated pipette was placed separately in each of the test tubes and extracted 1ml of the solutions into it. Once the mixture was in the pipette, someone from the group placed a piece of parafilm securely on the open end of the pipette and upon completion removed the top part of the graduated pipette.
If I was to do this experiment again I might use a Fungi amylase to