Antibiotics Lab Report

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Kirby -Bauer Assay
Abstract:
In this Experiment, antibiotics will be applied to the bacteria to find out which antibiotic will be most effective in controlling the growth of the bacteria by comparing it to the control group.

Introduction:
This lab will help figure out which antibiotic is most effective in hindering the bacterial growth by measuring the zones of inhibition. The antibiotic that creates the greatest zone of inhibition is the most effective antibiotic in controlling bacterial growth. To show this, the experimental groups, which receive the treatment of the different antibiotics, will later be compared to the control group, and whichever antibiotic shows the greatest zone of inhibition will be the most effective.

Hypothesis: …show more content…

Clean the work area effectively.
2. Obtain three TSA plates and label as in video.
3. Obtain an inoculate plate and observe the different colonies growing on the plate and record your observations. Note their appearance and other features.
4. Choose one bacterial colony for experiment.
5. Follow the procedure for the experiment as shown in the video make bacterial lawn on the plates and allow to site for a few minutes.
6. Label the quadrants on the media containing half of the petri dish and apply a single antibiotic disc in each of the three test areas and a blank in the fourth. Each of the Kirby-Bauer plates should have one disc of each antibiotic on it.
7. Seal the plates and place them in the designated area for the GTA to collect and incubate at 28 C for 24 hours. GTA will place plates in refrigerator to halt further bacterial growth.
8. Dispose of plates and other materials and directed by instructor.
Week 2
1. Clean the work area properly with sanitizers.
2. Measure the zones of inhibition for each antibiotic and record the data in notebook.
3. Compare results to the guidelines in Table 1. These standardized values indicate whether the bacteria is resistant, intermediately susceptible, or fully susceptible to the antibiotic based on the size of the zone of

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