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Staphylococcus aureus research paper
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Unknown Bacteria report: Staphylococcus Aureus
Staphylococcus aureus is the most known bacteria since the 1800s. It is derived from the commonly known genus staphylococci, which has over 20 different known species. Staphylococcus aureus species is the most dangerous of all the strains of staphylococcal bacteria. Staphylococcus aureus is the most common cause of staph infections and is the third most common cause of infections in the newborn nursery and surgical wards. [1] It is a primary cause of skin and soft tissue infections such as boils, furuncles, and cellulitis. The prominence of staphylococcus aureus as a serious health concern in hospitals, communities and jails has prompted extensive studies of the bacteria.
Staphylococcus aureus is a gram-positive coccal bacterium that is a member of the Firmicutes, and is frequently found in the nose, respiratory tract, and on the
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The history of Staphylococcus aureus was first observed in 1871 by von Recklinghausen but not isolated. In 1884, Rosenbach successfully isolated and grew Staphylococcus aureus from pus. Rosenbach is credited with proposing the genus Staphylococcus and the species Staphylococcus aureus.[3] Under a microscope, Staphylococcus aureus looks like an irregular cluster of grapes.
Staphylococcus aureus produces round, opaque colonies and can grow at any temperature between 10 and 46 degrees Celsius. It grows well with the help of oxygen and carbon dioxide. Most strains are metabolically versatile where they digest proteins and lipids and ferment a variety of sugars. Staphylococci are facultative anaerobes that grow by aerobic respiration or by fermentation that produces lactic acid. The species is considered among the most resistant of all non-spore forming pathogens. It stays alive after months of air drying and
...indole, it is motile, there is no urease present and there is no coagulase activity. By deduction and logical reasoning Unknown 10a was determined to be Escherichia coli.
In this lab project, the microbiology students were given 2 unknown bacteria in a mixed broth each broth being numbered. The goal of this project is to determine the species of bacteria in the broth. They had to separate and isolate the bacteria from the mixed broth and ran numerous tests to identify the unknown bacteria. The significance of identifying an unknown bacteria is in a clinical setting. Determining the exact bacteria in order to prescribe the right treatment for the patient. This project is significant for a microbiology students because it gives necessary skills to them for future careers relating to clinical and research work.
The purpose of this laboratory is to learn about cultural, morphological, and biochemical characteristics that are used in identifying bacterial isolates. Besides identifying the unknown culture, students also gain an understanding of the process of identification and the techniques and theory behind the process. Experiments such as gram stain, negative stain, endospore and other important tests in identifying unknown bacteria are performed. Various chemical tests were done and the results were carefully determined to identify the unknown bacteria. First session of lab started of by the selection of an unknown bacterium then inoculations of 2 tryptic soy gar (TSA) slants, 1 nutrient broth (TSB), 1 nutrient gelatin deep, 1 motility
Staphylococcus epidermidis is classified as a bacterium. This particular bacterium is identified in laboratory testing by its forms of clusters and a violet stain. The violet stain indicates that the staphylococcus epidermidis is gram- positive meaning that it is sphere shaped and does not contain an outer cell membran). This type of Staphylococcus does not typically cause disease in healthy people. However, S. epidermidis is quite the opportunist.
In the late 1880s, genes, white blood cells, and aspirin were discovered. An Augustinian monk from Austria, Johann Gregor Mendel experimented in the crossplanting of pea plants. Eventually his research lead to the discovery of genes. In 1892, Elie Metchnikoff discovered phagocytosis. After observing the larvae of starfish, he found that mobile cells served as a defense for the organisms. This research on the cells lead him to believe that these cells swallow up and digest bacteria, therefore leading into the identification of white blood cells. Although it is unclear who deserves credit for the discovery of aspirin, Felix Hoffman and Heinrich Dreser are credited for the introduction. Both of them researched the drug while working for Bayer and they are credited for actually naming it "aspirin".
In the last decade, the number of prescriptions for antibiotics has increases. Even though, antibiotics are helpful, an excess amount of antibiotics can be dangerous. Quite often antibiotics are wrongly prescribed to cure viruses when they are meant to target bacteria. Antibiotics are a type of medicine that is prone to kill microorganisms, or bacteria. By examining the PBS documentary Hunting the Nightmare Bacteria and the article “U.S. government taps GlaxoSmithKline for New Antibiotics” by Ben Hirschler as well as a few other articles can help depict the problem that is of doctors prescribing antibiotics wrongly or excessively, which can led to becoming harmful to the body.
Bacteria play a large role in our health, the environment, and most aspects of life. They can be used in beneficial ways, such as decomposing wastes, enhancing fertilizer for crops, and breaking down of substances that our bodies cannot. However, many bacteria can also be very harmful by causing disease. Understanding how to identify bacteria has numerous applications and is incredibly important for anyone planning to enter the medical field or begin a career in research. Having the background knowledge of identifying an unknown bacteria may one day aid healthcare professionals diagnose their patient with a particular bacterial infection or help researchers determine various clinical, agricultural, and numerous other uses for bacteria.
This pathogen, Streptococcus pneumoniae, is a gram-positive coccus that is long shaped and usually seen in groups of pairs (Todar, 2008-2012). This pathogen ranges from o.5-1.25 micrometers, which is pretty small in size (Todar, 2008-2012). It “lacks catalase and ferments glucose into lactic acid” (Todar, 2008-2012). To grow this bacterium in the lab the best way to do it would be to grow it on a blood agar at 37 degrees Celsius and produces a green zone arou...
those that correlate to the flesh-eating bacteria now known as streptococcus pyogenes. Due to Hippocrates’ acts as a pioneer in the medical field, these chronicles are the first recordings we have of the existence of the bacteria.
Life History and Characteristics: Staphylococcus aureus is a gram positive bacterium that is usually found in the nasal passages and on the skin of 15 to 40% of healthy humans, but can also survive in a wide variety of locations in the body. This bacterium is spread from person to person or to fomite by direct contact. Colonies of S. aureus appear in pairs, chains, or clusters. S. aureus is not an organism that is contained to one region of the world and is a universal health concern, specifically in the food handling industries.
Staphylococcus aureus is a bacteria that is abundant in many places. It can even be found in some of our bodies. These bacteria are harmless as long as none of them are Methicillin resistant Staphylococcus aureus (MRSA). Methicillin is the name of a family of antibiotics that includes penicillin. This MRSA is the deadly superbug that has developed resistant to antibiotics. Statistics show that MRSA contributes to more US deaths than does HIV. It has become a huge threat to every country as the outbreaks can be a surprising one. This threat is caused by the evolution of the bacteria. These superbugs have evolved a resistance of antibiotics which makes them extremely difficult to treat. One article states, “In the early 1940s, when penicillin was first used to treat bacterial infections, penicillin-resistant strains of S. aureus were unknown — but by the 1950s, they were common in hospitals. Methicillin was introduced in 1961 to treat these resistant strains, and within one year, doctors had encountered methicillin-resistant S. aureus. Today, we have strains of MRSA that simultaneously resist a laundry list of different antibiotics, including vancomycin — often considered our last line of antibacterial defense.” [1]
The principle sites of nosocomial infections in patients, in order from most common to least common are: urinary tract, surgical wounds, respiratory tract, skin, blood, gastrointestinal tract, and central nervous system (Abedon). According to the CDC, the most common pathogens that cause nosocomial infections are Staphylococcus aureus, Pseudomonas aeurginosa, and Escherichia coli (EHA). Methicillin resistant Staphylococcal aureus (MRSA) is a strain of bacteria that is commonly...
Resistant strains are no joke, for years my mother has been dealing with MRSA (methicillin-resistant staphylococcus aureus). My mother has had MRSA so bad that at one time she was covered in MRSA sores much like when a person breaks out in hives. Watching her suffer the way she has worries me as I am also a healthcare worker and know what these resistant strains are capable of. I know these strains exist because of a lack of proper patient education. Proper patient education is key to the use of antibiotics and prevention of resistance-bacteria strains.
Bacterial cells, like plant cells, are surrounded by a cell wall. However, bacterial cell walls are made up of polysaccharide chains linked to amino acids, while plant cell walls are made up of cellulose, which contains no amino acids. Many bacteria secrete a slimy capsule around the outside of the cell wall. The capsule provides additional protection for the cell. Many of the bacteria that cause diseases in animals are surrounded by a capsule. The capsule prevents the white blood cells and antibodies from destroying the invading bacterium. Inside the capsule and the cell wall is the cell membrane. In aerobic bacteria, the reactions of cellular respiration take place on fingerlike infoldings of the cell membrane. Ribosomes are scattered throughout the cytoplasm, and the DNA is generally found in the center of the cell. Many bacilli and spirilla have flagella, which are used for locomotion in water. A few types of bacteria that lack flagella move by gliding on a surface. However, the mechanism of this gliding motion is unknown. Most bacteria are aerobic, they require free oxygen to carry on cellular respiration. Some bacteria, called facultatibe anaerobes can live in either the presence or absence of free oxygen. They obtain energy either by aerobic respiration when oxygen is present or by fermentation when oxygen is absent. Still other bacteria cannot live in the presence of oxygen. These are called obligate anaerobes. Such bacteria obtain energy only fermentation. Through fermentation, different groups of bacteria produce a wide variety of organic compounds. Besides ethyl alcohol and lactic acid, bacterial fermentation can produce acetic acid, acetone, butyl alcohol, glycol, butyric acid, propionic acid, and methane, the main component of natural gas. Most bacteria are heterotrophic bacteria are either saprophytes or parasites. Saprophytes feed on the remains of dead plants and animals, and ordinarily do not cause disease. They release digestive enzymes onto the organic matter. The enzymes breakdown the large food molecules into smaller molecules, which are absorbed by the bacterial cells. Parasites live on or in living organisms, and may cause disease. A few types of bacteria are Autotrophic, they can synthesize the organic nutrients they require from inorganic substances. Autotrophic bacteria are either photosynthetic or Chemosynthetic. The photosynthetic bacteria contain chlorophyll that are different from the plant chlorophyll. In bacterial photosynthesis, hydrogen is obtained by the splitting of compounds other than water.