TLC Analysis Of Analgesics

421 Words1 Page

TLC Analysis of Analgesics Due to the versatility and inexpensiveness of thin layer chromatography, it is one of the most widely used techniques in laboratories today. Thin layer chromatography has many uses in the realms of pharmaceuticals, forensics, industry and most especially organic chemistry. More specifically, the main applications of TLC are to detect the presence of a particular compound in urine or blood, the ingredients and/or chemical composition of anything ranging from food to drugs, and plants to explosives. The way chemical compositions are analyzed via TLC by establishing the number of compounds or elements that exist in a reaction, whether or not the compounds are different and to check the progress of the reaction, which is determined based on the …show more content…

The components of an analgesic will be determined by noting the separation between the solid and liquid (or mobile) phases and comparing it to these predicted reference values. Some samples may contain more than one of these analgesic compounds and therefore have dots corresponding at multiple Rf values. However it is not only the chemical components that influence the Rf value. The eluting solvent mixture also has an effect. For instance, changing the solvent mixture can alter the Rf value and separation between the mobile/liquid phase and solid phases. For instance, the greater the polarity of the eluting solvent (ie methanol) the more polar the analyze, the slower it will move up the plate since the polar solvents will dissolve the polar analytics more effectively and move the analyses up the TCL plate at a faster rate. The more polar the solvent, the quicker the analyst will move. This means that we will get little separation on the plates. The opposite can be predicted for non-polar solvents such as

More about TLC Analysis Of Analgesics

Open Document