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Effect of sugar concentration on fermentation
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The question to be answered in this experiment is, “How does different sugars and sugar substitutes affect the rate of fermentation in yeast?” This question requires background knowledge on yeast, sugars/sugar substitutes, and fermentation.
Fermentation is the process that an organism goes through to either create lactic acid or ethanol and CO2. This is done without the use of oxygen, also known as anaerobic respiration. There are multiple steps of both fermentation types. They both begin with the glycolysis, where 1 glucose molecule is broken down into 2 pyruvic acid molecules and 2 ATP molecules. From this step the two feremenation types then branch off. In alcoholic fermentation, pyruvic acid and NADH is converted to 2 ethanol, NAD+, and 2CO2. In lactic acid fermentation, pyruvic acid and NADH is converted to 2 lactic acids and NAD+. Yeast is a very critical portion of this experiment. Yeast is a eukaryotic (has a nucleus) unicellular organism. It is a fungi that is commonly used for baking, and alcohol production. This is because yeast undergoes alcoholic fermentation. Because of this it has the products of CO2 and ethanol.
To understand how the different types of sugar may affect fermentation, knowledge on the sugars is essential. Additionally, glucose is
This is because cane sugar has a lot calories which means it has energy, energy is created by cellular respiration with glucose and oxygen. If cane sugar has energy then it has to have glucose, as mentioned before, yeast feeds on simple sugar like glucose to go through fermentation, thus since the cane sugar has the materials for fermentation in abundance, then it will have a higher rate of fermentation than the sweeteners which have no calories. If it has no calories then it has no energy and thus no glucose, nothing for the yeast to use in
Fermentation is the biological process which allows humans to brew beer, or any other alcoholic beverage. This process occurs in the absence of oxygen, as a means for the cell to produce adenosine triphosphate (ATP), the source of cellular energy. Though little energy can be produced in this manner, it allows the yeast to survive in t...
The Effects of Concentration of Sugar on the Respiration Rate of Yeast Investigating the effect of concentration of sugar on the respiration rate of yeast We did an investigation to find how different concentrations of sugar effect the respiration rate of yeast and which type of concentration works best. Respiration is not breathing in and out; it is the breakdown of glucose to make energy using oxygen. Every living cell in every living organism uses respiration to make energy all the time. Plants respire (as well as photosynthesise) to release energy for growth, active uptake, etc…. They can also respire anaerobically (without oxygen) to produce ethanol and carbon dioxide as by-products.
The purpose of this investigation is to test the effects of multiple sugar substances on the respiration of yeast. Most people think of yeast when they think of what makes bread rise, cheese, alcoholic beverages, or other food products. Another type of yeast can also cause yeast infections, an infection of the skin. Yeasts (Saccharomyces) are tiny, microscopic organisms with a thin membrane and are usually oval or circular-shaped. They are a type of single-celled fungi of the class Ascomycetes, capable of processing sugar into alcohol and carbon dioxide (CO2 ) ; this process is known as fermentation. Fermentation and the products are the main focus points for this experiment being that cellular respiration of yeasts happens via the process of fermentation, which creates by-products of alcohol and CO2. The level of CO2 produced by the yeasts will show how effective each sugar substance is in providing cellular energy for the yeasts.
Paturau, J.M. 1982. By-products of the cane sugar industry. Second ed. Elsevier scientific Publishing Co., New York. 366 pp.
1. If gas bubbles form then fermentation occurred. Glucose. Carbon dioxide. The enzyme didn’t recognize the structure of glactose, because of the orientation of the H and OH on the carbon 4 is different than glucose. The enzyme only identifies very specific substances.
Fermentation is a form of chemical transformation of organic substances that breaks down simple compounds by exploiting the enzymes with compl...
Initially, all people think of sugar in foods as a sweetener, but what they do not recognize is that it is used to preserve food. Such as: jams and canned fruits. Also sugar thickens the texture that makes liquid much better. Sugar is used around the world for bakery products and soft drinks. Furthermore, sugar is used in pharmaceutical industries, it is a common knowledge that sugar is a medicine that treats people with low blood sugar, also for diabetics with insulin intake; to balance sugar levels. In addition, sugar is a rehydration source that prevents dehydration. Additionally, sugar is used in the production of fabrics. In summary, sugar without a doubt is an important commodity because of these uses and because of its
This lab attempted to find the rate at which Carbon dioxide is produced when five different test solutions: glycine, sucrose, galactose, water, and glucose were separately mixed with a yeast solution to produce fermentation, a process cells undergo. Fermentation is a major way by which a living cell can obtain energy. By measuring the carbon dioxide released by the test solutions, it could be determined which food source allows a living cell to obtain energy. The focus of the research was to determine which test solution would release the Carbon Dioxide by-product the quickest, by the addition of the yeast solution. The best results came from galactose, which produced .170 ml/minute of carbon dioxide. Followed by glucose, this produced .014 ml/minute; finally, sucrose which produced .012ml/minute of Carbon Dioxide. The test solutions water and glycine did not release Carbon Dioxide because they were not a food source for yeast. The results suggest that sugars are very good energy sources for a cell where amino acid, Glycine, is not.
Sugarcane is an important industrial crop for the tropical and subtropical region of the world. It is produced in more than 100 countries, with global production of 174 million tonnes sugar. It accounts for about 80 percent and sugarbeet for about 20 percent of total sugar produced (FAOSTAT, 2008). In 2010, 1,682 million metric tonnes (MT) of sugarcane were produced worldwide in a total area of 23.8 million hectares (ha). Brazil is the largest sugarcane producer, contributing with 40% of the world production (719 MT) followed by India (278 MT), China (111 MT), Thailand (68 MT), Pakistan (50 MT), Colombia (38.5 MT), Australia (31 MT), Argentina (30 MT), United States (27.5 MT), Indonesia (26.5 MT) and the Philippines (23 MT) (FAOSTAT, 2011). India rank second among the sugarcane growing countries of the world in both area and production. Globally it is cultivated over an area of 20.1 million hectare, with annual production of 1381.1 million tonnes and productivity of 65.5 tonnes per ha. In India sugarcane is cultivated over an area of 4.36 million ha, with an annual production of 281.8 million tonnes and productivity 64.6 tonnes per ha. Uttar Pradesh, Maharashtra, Karnataka, Tamilnadu and Andhra Pradesh are the important sugarcane
and lysis, meaning splitting [8]. In this process a molecule of glucose is degraded into two molecules of pyruvate. Glycolysis consists of ten enzyme catalysed
Further, fermented food itself is interpreted by Campbell-Platt (1987) “as those foods which have been subjected to the action of micro-organisms or enzymes so that desirable biochemical changes cause significant modification to the food.” (As cited in Sahlin, 1999, p.5)
Not all sugars are made the same. Sugar is naturally found in many foods like fruits, vegetables, and dairy. It is also an added ingredient in many processed foods like soups, condiments, and beverages. The sugars added to foods tend to be highly concentrated and devoid of other nutrients. In contrast, natural sugars are integrated into
Yeasts are facultative anaerobes. They are able to metabolize the sugars in two different ways which is aerobic respiration in the presence of oxygen and anaerobic respiration in the absence of oxygen. The aerobic respiration also known as cellular respiration takes place when glucose is broken down in the present of oxygen to yield carbon dioxide, water and energy in the form of ATP. While in anaerobic respiration, fermentation takes place because it occurs in the absence of external electron acceptor. Because every oxidation has to be coupled to a reduction of compound derived from electron donor. On the other hand, in cellular respiration an exogenous
According to Rahman (2007), fermentation is one of the earliest ancient traditions developed by cultures all around the world to prolong the possible storage time of foods. Moreover, it is a process in which microorganisms alter the sensory and functional properties of a food in order to come up with a desirable product for the consumer. It is also the process by which the chemical changes are brought about in an organic substrate or organic materials by means of the action of a biological catalyst known as enzyme.
The process of alcoholic fermentation begins with the use of enzymes. The enzymes begin to break down the long chains in starch molecules, a polysaccharide that consists of a large quantity of glucose molecules (C6H12O6) joined by glycosidic bonds as seen in figure 1, into single glucose molecules, a monosaccharide with six carbons and five hydroxyl groups. After the starch has become sugar, the enzymes are used once again, this time to convert the sugars into ethyl alcohol and carbon dioxide, CO2, as seen in figure 2 (World of Scientific Discovery, 2007). The carbon dioxide produced is released into the atmosphere, leaving water and ethanol, the alcohol, behind. Ethanol is a colorless flammable liquid with a molecular formula of C2H6O, giving it a molar mass of 46.07 grams per mole. Ethanol is also characterized by a melting point of -114°C or 159 K.