The results verified that the spinach had the highest chloroplasts concentration because it had the absorbency of 0.730, 0.826, 1.011, and 1.049 as shown in Table 1-4. The spinach was our positive control because, from a previous experiment, we knew that it would have chloroplasts; the buffer sucrose was the negative control because if the cabbage or lettuce did not have any contents of chloroplasts, then the readings should be equivalent to the buffer sucrose since it was our blank solution. Therefore, it should read 0.00, if there were no contents of chloroplasts. On the other hand, there was a flux in the data between the cabbage and the lettuce. However, the overall data suggested that the cabbage had a higher content of chloroplasts than …show more content…
The chloroplasts within the spinach were triggered by the light in the room, as the function of chloroplasts is to induce photosynthesis. A similar experiment was tested by Clapper and Sallee. They concluded that the rate of photosynthesis of the spinach solution was greater than it was in both the cabbage and lettuce. Clapper and Sallee stated that the red cabbage and lettuce may have had slower growth rates, which was why the chloroplasts concentration plateaued after 5 minutes from the first reading. In Table 4, we tested the concentration of each plant at a wavelength of 500nm to see if we’d get a better reading. The same results showed that spinach had the highest concentration of chloroplasts and lettuce had the least concentration. In spite of it all, there has been debate over the accuracy of calibrating the chloroplasts concentration through a spectrophotometer because some scientists argued that low contents of chlorophyll a were more sensitive at a wavelength of 675nm compared to a wavelength at 550nm (Xue and Yang,
Two members of the group were instructed to visit the laboratory each day of the experiment to water and measure the plants (Handout 1). The measurements that were preformed were to be precise and accurate by the group by organizing a standardized way to measure the plants. The plants were measured from the level of the soil, which was flat throughout all the cups, to the tip of the apical meristems. The leaves were not considered. The watering of the plants took place nearly everyday, except for the times the lab was closed. Respective of cup label, the appropriate drop of solution was added to the plant, at the very tip of the apical meristems.
The primary nutrients that plants require are carbon, hydrogen, and oxygen from air and water. Sugar has a different effect on animals, humans, and plants. In this experiment, we are testing the effects of sugar on the growth of wheatgrass. For this experiment, we used regular sugar that we put in our food and drinks. To test our hypothesis we are using two groups control and experiment group. The control plant received only water but the experimental plant received sugar but, both plants were placed in the same temperature and same amount of water. Our hypothesis was correct, experiment plant “sugar water” yield more plant growth than control plant “water”. This experiment shows the sugar water plant grow faster than water because of the average of both plants. The sugar water experiment plant had longer in length compared to water control plant.
To test for this, DCIP (a chloroplast isolation buffer) was used to The hypothesis for this experiment was that the cell fraction in the cuvette marked P2 will have more chloroplast activity because it will exhibit greater color change and differences in the absorbance readings compared to the other cuvettes when exposed under the condition of light; moreover, this notion was believed to be so because the more a cell fraction is centrifuged, the more intact chloroplasts we’ll find (Leicht and McAllister, This meant that this cuvette (tested under light) should display a higher decrease in DCIP due to the reduction in absorbance (dependent variable) opposed to the other cell fractions tested depending on a sixteen minute period (independent variable). The overall goal was to provide proof, through data, that the cell fractions put under the light during the sixteen minute period would indicate a higher set of chloroplast activity versus the ones put in the
The “Fast Plant” experiment is an observation of a plants growth over the span of twenty-eight days. The objective is to observe how plants grow and use their resources throughout the span of their life. In our lab we observed the Brassica rapa, a herbaceous plant in the mustard family which has a short cycle which makes it a perfect plant to observe in this experiment. Like other plants the Brassica rapa must use the resources in the environment to create energy to complete itʻs life cycle and reproduce. By observing the plant it is easy to see in what organ or function the plant is using itʻs energy and resources and if overtime the resources switch to other part of the plants. By conducting this experiment we are able to observe where and how plants allocate their resources throughout their life by harvesting plants at different points in their life.
... in the chloroplasts in some of their cells. Chlorophyll allows the energy in sunlight to drive chemical reactions. Chloroplasts act as energy transducers, converting light energy into chemical energy. So as the plant has more light the chlorophyll inside the chloroplasts can react faster absorbing in more light for food and energy.¡¨ So this shows my prediction was correct for in my experiment and shown in my result table and graph the more light intensity there is on a plant the higher the rate of my photosynthesis will be. My prediction is very close to what I said the results will be so my prediction was correct and has been proven to be correct in my result table, graph and now explained again in my conclusion.
ABSTRACT: Chloroplasts carry out photosynthetic processes to meet the metabolic demands of plant cells (Alberts, 2008). They consist of an inner thylakoid membrane and a stroma. (Parent et. al, 2008).In this experiment we demonstrate the unique protein compositions of isolated thylakoid and stromal fractions from broken and whole spinach chloroplasts. Because these compartments carry out different metabolic processes, we confirm our hypothesis that performing SDS-PAGE on these fractions will result in distinct patterns on the gels. In isolating and analyzing nucleic acid from broken, whole, and crude chloroplast samples we demonstrate that genes for photosynthetic protein psbA are found in chloroplast DNA, while genes for photosynthetic enzyme
... has more CO2 than the negative controls. There will be more spinach circles in the red food coloring than the green and blue food coloring. My hypothesis was supported for both experiments.
The greater overall rate of absorbance change in all chloroplast samples (Figure 1) confirms role of chloroplasts’ in photosynthesis. However, the use of the supernatant sample as a negative control was expected to yield no activity, which was shown to be untrue (Figure 1) and is contributed to the contamination of the supernatant sample with chloroplast. The fragile envelope of the chloroplasts can be eas...
= > [CH2O} + O2 + H2O, This shows that when the light intensity is increased the rate of reaction will be more quicker he only anomalous result there was, is the one in the 100 watt result the reading after 5 minutes is anomalous because it does not follow the predicted pattern of increasing in the production of gas because it is lower I know from my own knowledge of photosynthesise that when the light intensity is increased the rate of reaction will be more quicker because many plants and trees photosynthesise quicker in stronger light and photosynthesise slower in dimly lit places. The chlorophyll absorbs light energy and enables it to be used by the plant for building up sugar. The overall effect is that energy is transferred from sunlight to sugar molecules.
The Effect of Wavelength on Photosynthesis Rate Aim: To be able to To investigate how different wavelengths (colors) of light affect the photosynthetic rate of the synthetic. I will use a pant that is a pond weed called elodea. I will measure the rate of photosynthesis by measuring the amount of o2 given off in bubbles per minute from the elodea. I will do this by placing the Elodea in a test tube with sodium hydrogen. carbonate then I will vary the light wavelength (color) using colored.
In this laboratory experiment, the rate of photosynthesis was measured through the use of the “floating leaf disk technique.” The leaf disks were placed into a syringe and the O2 and CO2 in the mesophyll layers of the leaves were removed and then replaced with sodium bicarbonate or water, causing the leaves to sink to the bottom of the container. If one determines the number of leaf disks rising to the top as a result of an increase in oxygen gas in the mesophyll cells, then the rate of photosynthesis is able to be measured because O2 is a product of photosynthesis. The first step of this experiment was a feasibility study of the variance in the photosynthetic activity of the leaf disks in both water and bicarbonate solutions. After five minutes of light exposure, all of the leaf disks in the bicarbonate solution (10 disks) had ...
The Effect of Light Intensity on Photosynthesis Of Elodea Canadensis Introduction I wanted to find out how much the light intensity affected the Photosynthesis in Elodea Camadensa. I decided to do this by measuring the amount of oxygen created during photosynthesis. Photosynthesis is the procedure all plants go through to make food. This process uses Carbon dioxide, water and light energy. It produces Oxygen and Glucose.
The Effect of Light Intensity on the Rate of Photosynthesis in an Aquatic Plant Introduction The input variable I will be investigating is light, as light is just one of the 4 factors required in the green-plant process of photosynthesis. Photosynthesis is the process by which green-plants use sunlight, carbon dioxide, water & chlorophyll to produce their own food source. This process is also affected by the temperature surrounding the plant (the species of plant we experimented with, pond weed, photosynthesised best at around 20 degrees centigrade.) Light, temperature & CO2 are known as limiting factors, and each is as important as the next in photosynthesis. Light is the factor that is linked with chlorophyll, a green pigment stored in chloroplasts found in the palisade cells, in the upper layer of leaves.
In our Biology Lab we did a laboratory experiment on fermentation, alcohol fermentation to be exact. Alcohol fermentation is a type of fermentation that produces the alcohol ethanol and CO2. In the experiment we estimated the rate of alcohol fermentation by measuring the rate of CO2 production. Both glycolysis and fermentation consist of a series of chemical reactions, each of which is catalyzed by a specific enzyme. Two of the tables substituted some of the solution glucose for two different types of solutions. They are as followed, Table #5 substituted glucose for sucrose and Table #6 substituted the glucose for pH4. The equation for alcohol fermentation consists of 6 Carbons 12 Hydrogens 6 Oxygen to produce 2 pyruvates plus 2 ATP then finally the final reaction will be 2 CO2 plus Ethanol. In the class our controlled numbers were at Table #1; their table had 15 mL Glucose, 10 mL RO water, and 10 mL of yeast which then they placed in an incubator at 37 degrees Celsius. We each then measured our own table’s fermentation flasks every 15 mins for an hour to compare to Table #1’s controlled numbers. At
An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis. Introduction Photosynthetics take place in the chloroplasts of green plant cells. It can produce simple sugars using carbon dioxide and water causing the release of sugar and oxygen. The chemical equation of photosynthesis is: [ IMAGE ] 6CO 2 + 6H20 C 6 H12 O 6 + 6O2 It has been proven many times that plants need light to be able to photosynthesize, so you can say that without light the plant would neither photosynthesize nor survive.