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Application of industrial enzymes
Application of industrial enzymes
Application of industrial enzymes
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Rennin – A Proteolytic Enzyme
Major Discussion:
Role of rennin in helping young mammals digest milk
Role of rennin as an industrial catalyst to make cheese
Proteolytic Enzyme:
Any group enzymes that catalyze the hydrolytic degradation of proteins or polypeptides to smaller amino acids polymer is called proteolytic enzyme.
Rennin is a proteolytic enzyme made by chief cells in the stomach. Rennin also known as “Chymosin”. Rennin enzyme plays an important role in helping young mammal digest milk. Rennin is also used as industrial catalyst to make cheese.
Role of rennin in helping young mammals digest milk
In human babies, rennin is associated with the coagulation of milk. This property help in the digestion of milk in infants. Rennin,
This key function is carried out by chymosin/rennin. Interestingly, the conversion of milk into yogurt is possible only at 37 degree Celsius, which is the normal human body temperature.
Thus, chymosin plays a vital role in the nutrition of young mammals.
Rennin and renin: Rennin is sometimes also mistaken with renin, an enzyme secreted by kidneys that takes part in the regulation of arterial blood pressure. Thus, rennin and renin are different, and the two terms should not be used interchangeably.
Role of rennin as an industrial catalyst to make cheese
Introduction:
Chymosin is a very important industrial enzyme because it is broadly used in cheese production. Thousands of years ago, farmers and shepherds made a mistake. For the sake of transporting their drinking milk, people stored it in sacks made from the sheep’s stomach. After being stored for some time, it was found that the product was no longer milk, but instead it separated into solids and liquid, or curds and whey. Because of this mistake the use of animal rennet in cheese production entered the collective stream of consciousness, and made to become the practice still routinely used
Living organisms undergo chemical reactions with the help of unique proteins known as enzymes. Enzymes significantly assist in these processes by accelerating the rate of reaction in order to maintain life in the organism. Without enzymes, an organism would not be able to survive as long, because its chemical reactions would be too slow to prolong life. The properties and functions of enzymes during chemical reactions can help analyze the activity of the specific enzyme catalase, which can be found in bovine liver and yeast. Our hypothesis regarding enzyme activity is that the aspects of biology and environmental factors contribute to the different enzyme activities between bovine liver and yeast.
One of the most primitive actions known is the consumption of lactose, (milk), from the mother after birth. Mammals have an innate predisposition towards this consumption, as it is their main source of energy. Most mammals lose the ability to digest lactose shortly after their birth. The ability to digest lactose is determined by the presence of an enzyme called lactase, which is found in the lining of the small intestine. An enzyme is a small molecule or group of molecules that act as a catalyst (catalyst being defined as a molecule that binds to the original reactant and lowers the amount of energy needed to break apart the original molecule to obtain energy) in breaking apart the lactose molecule. In mammals, the lactase enzyme is present
Enzyme action Rennin A proteolytic enzyme that speeds up the coagulation of milk. It is usually found in the tissues of a calves fourth stomach. Its purpose is to coagulate the milk in young animals so that the proteins have time to be extracted, rather than flowing straight through the digestive system. This particular enzyme catalyses the conversion of the protein in milk (caseinogen) into paracasein. This forms a thick curd in the stomach meaning the milk can be exposed to Rennin for a greater period of time.
The Effect of Temperature on the Activity of Rennin in Milk Aim: To find out what effect different temperatures have on the enzyme, rennin, in milk. Introduction An enzyme is a biological catalyst. It speeds up a reaction by lowering the activation energy required to start the reaction. It speeds up a reaction, but remains unchanged unless certain limiting factors are introduced.
While the tube for specimen Cb turned a tannish white in the lower half of the tube while the top stayed the lavender inoculated tube color. Do to this evidence I determined that both specimens Ca and Cb cannot use the process Casein hydrolysis or Casein coagulation due to lack of soft or firm curds in both tubes. Since there was no casein curds formed, I concluded that specimens Ca and Cb also cannot perform the process of proteolysis. My conclusion is supported by the fact that there was no clearing of the medium. I have also determine that neither of my organisms can make the enzymes rennin, proteolytic or even proteases. I know my specimens cannot produce proteases due to the fact that there was no blue coloring in the tubes which means that the byproduct Ammonia was not produced to increase the pH. Since neither of my specimens can make these enzymes, I concluded that my specimens cannot break down lactose or casein. Although I did learn that specimen Cb can reduce litmus due to the evidence that the lower part of the tube turned a tannish white color with a purple ring at the top. This color change from a purple to a white means that the litmus was reduced turning it clear and leaving the white of the milk to show. Finally I know that specimen Ca cannot reduce litmus due to the fact that the tube had no change in
The shape of the molecules is changing and so the enzyme molecules can no longer fit into the gaps in the substrate that they need to and therefore the enzymes have de – natured and can no longer function as they are supposed to and cannot do their job correctly. Changing the temperature: Five different temperatures could be investigated. Water baths were used to maintain a constant temperature. Water baths were set up at 40 degrees, 60 degrees and 80 degrees (Celsius). Room temperature investigations were also carried out (20 degrees).
Lactase is an enzyme found in the digestive system. It is essential to the complete digestion of sugar in whole milk and milk products. Lactase specifically breaks down lactose, a complex sugar. Lactase cannot be absorbed by the body unless it is broken down by lactase into glucose and galactose. According to webMD, “Lacking lactase in their intestines, a person consuming dairy products may experience the symptoms of lactose intolerance…Abdominal cramping, flatulence (gas) and diarrhea can occur when a lactose intolerant person consumes milk products.” ("Lactase Enzyme oral : Uses, Side Effects, Interactions, Pictures, Warnings & Dosing - WebMD", n.d.) Lactase is not recommended for use in CHILDREN younger than 4 years of age. Safety and effectiveness in this age group have not been confirmed. (Kluwer, 2014)
Proteins are one of the main building blocks of the body. They are required for the structure, function, and regulation of the body’s tissues and organs. Even smaller units create proteins; these are called amino acids. There are twenty different types of amino acids, and all twenty are configured in many different chains and sequences, producing differing protein structures and functions. An enzyme is a specialized protein that participates in chemical reactions where they serve as catalysts to speed up said reactions, or reduce the energy of activation, noted as Ea (Mader & Windelspecht).
...at keep organisms alive. “Proteins are the most structurally sophisticated molecules known” (Campbell, 1999) which is reason enough to study them. The techniques we learned in this lab form a basis from which a detailed study of proteins is possible. Following our procedure we were successfully able to set up a quantifying assay to determine the amount of protein within a milk sample, although our yield percentage was rather low. However, errors in this lab (in the form of a low yield percentage) may have an origin from our last lab. In the process of extracting proteins from the milk sample, we may have inadvertently lost some of the protein through erroneous measurements, or perhaps through poor handling of either ammonium sulfate or the dialysis tubing. While not sufficient enough (at this point) to invalidate our results, they do explain the major difference between the expected and the actual amount of protein extracted.
Although the milk itself does not have a very long life, other foods and some dairy products can be made using it. Cheese would be the main example of this, which can be produced simply by the curdling of milk. Rennin, found in the substance rennet, is a milk-coagulating enzyme capable of assisting in the production of cheese. Therefore the temperatures at which the milk and rennet coagulate best at in this experiment, are
The exocrine function of the pancreas is that it produces enzymes that aids in the digestion of food. There are three important enzymes that are crucial in helping with digestion. The first digestive enzyme is amylase. Amylase function is to break down carbohydrates. The amylase enzyme is made in two places: the cells in the digestive tract that produces saliva and the main one specifically found in the pancreas that are called the pancreatic amylase (Marie, Joanne; Media Demand, “What Are the Functions of Amylase, Protease and Lipase Digestive Enzymes”). The amylase in the pancreas passes through the pancreatic duct to the small intestines. This amylase in the pancreas completes the process of digestion of carbohydrates. Consequently, this leads to the production of glucose that gets absorbed into the bloodstream and gets carried throughout the body. The next enzyme that aids in digestion of food is protease. While amylase breaks down carbohydrates, protease breaks down protein. Protease breaks down protein into the building block form of amino acids. The three main proteases that it produces are: pepsin, trypsin and chymotrypsin (Marie, Joanne; Media Demand, “What Are the Functions of Amylase, Protease and Lipase Digestive Enzymes”). Pepsin does not occur in the pancreas but it is the catalysis in starting the digestion of proteins. Trypsin and chymotrypsin are the two proteases that occur in
When eaten, protein is broken down into amino acids. Proteins and amino acids are used for almost every metabolic process in the body, and are the building blocks for every tissue in your body.
Wagner, C. L., Anderson, D. M., & Pittard III, W. B. (June 1996). Special properties of human milk. Clinical Pediatrics , p 283.
Its many contains disulphide bonds, which make it an extremely stable protein. References Website’s used :. www.Intelihealth.com - www. Inteli www.dentistry.leeds.ac.uk/biochem/lectures/nutrition. www.healthy.net/library/books/haas/funct.htm.
Breast milk matches a baby’s needs and growth patterns better than any formula substitute. A mother tends to produce just enough milk to fill her baby’s stomach, so she does not need to worry about underfeeding or overfeeding. Furthermore, the milk’s content changes as the ...