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Escribe an experiment to investigate osmosis in plant cells
Escribe an experiment to investigate osmosis in plant cells
Escribe an experiment to investigate osmosis in plant cells
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1. Restate your hypothesis and discuss if the observed results supported the hypothesis.
Hypothesis: I think that by combining the iodine and the starch the outcome would present itself as a dark color. I also assumed that the potatoes would soak up all the liquids.
2. Identify the control and variables in your experiment.
The manipulated variables are the changes in the development of the potatoes from the materials placed into the substances. The control variables are the pure water substance and the size of the potatoes. Those are the variables of the experiment.
Why do we observe the color of the solution before adding the sandwich bags to each cup?
We observe the color of the solution before adding the sandwich bags to each cup because
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The important mechanisms are diffusion, osmosis, and active and passive transport. Through simple diffusion, small noncharged molecules or lipid soluble molecules pass between the phospholipids to enter or leave the cell, moving from areas of high concentration to areas of low concentration. For polar compounds energy is needed in the form of ATP to enter the cell, this is called active transport.
7. List three criteria cells use to select materials to enter or leave the cell. Then explain the role of each criterion in determining the type of transport a cell will use for different sized molecules. For instance, small molecules move across the membrane by diffusion, given they are moving with the concentration gradient.
The three criteria are diffusion, osmosis, and active transport. Larger molecules move by facilitated diffusion if they are not charged and not lipid soluble, and very large molecules move by endo/exocytosis. Hydrophobic molecules are those that easily pass through the membrane by diffusion. These include those molecules which are soluble in lipids and are usually quite small. Hydrophilic molecules are often larger and have more trouble passing through the membrane. They need a transport protein to help them get through. Large molecules and those with a strong charge need energy, ATP, to help them get through the membrane. This is called active
Iodine is used to distinguish starch from mono/di/polysaccharides. In the test only two of the six solutions showed any signs of starch being present. The potato juice turned a brownish color with a precipitate indicating a slight presence of starch. The stach solution turned a dark blue/black color indicating a very high presence of starch in the solution. The presence of starch in the potato juice indicates that it does not have as high an amount of carbohydrates as onion juice, but a higher concentration of starch.
In the second experiment with the green color, I can safely conclude that the color green in this case is very soluble and we would need longer filter paper, perhaps more time to safely separate the different colors that make up the color green.
Conclusion In my conclusion, the potatoes with the lowest concentration gained the most mass, and would become hard relating back to the Turgor theory I stated earlier. In contrast to this, the potatoes in the most concentrated solution lost the most weight thus becoming plasmolysed and limp also relating back to the background I have mentioned earlier. Evaluation In general the experiment was succesful the results were consistent and also were in accordance with the theories made at the start.. The experiment could have been improved by: · More subjects used instead of potatoes · More potatoes · Wider time ranger · Different molarities Using this variety of methods could have improved the experiemnt, however I was generally satisfied with the results of this osmosis experiement.
All of these substances cross the membrane in a variety of ways. From diffusion and osmosis, to active transport the traffic through the cell membrane is regulated. Diffusion is the movement of molecules form one area of higher concentration to an area of lower concentration. Concentration gradient causes the molecules to move from higher concentration to a lower concentration.
Materials used in the experiment included 5-7 g of the potato tissue, 50ml of 2.0M phosphate buffer coffee filter and guaiacol dye.
When a cell membrane is said to be selectively permeable, it means that the cell membrane controls what substances pass in and out through the membrane. This characteristic of cell membranes plays a great role in passive transport. Passive transport is the movement of substances across the cell membrane without any input of energy by the cell. The energy for passive transport comes entirely from kinetic energy that the molecules have. The simplest type of passive transport is diffusion, which is the movement of molecules from an area of high concentration to an area of lower concentration. Diffusion
So the experiment will be based upon the movement of water. The first potato chip will be placed in a zero percent solution. I believe that this will increase the mass. I believe this because the zero percent solution has a lower concentration than the cell sap inside the potato. The potato takes on the water through osmosis and the cell pushes out from inside the cell making it swell and become more rigid.
An example of simple diffusion is osmosis. Facilitated diffusion on the other hand is dependant on carrier proteins to transport it across the membrane. Diffusion is essential for many organisms as it is a feature of a number of processes which control and supply vital substances to the body in order for basic survival. A few of these are discussed below. Gas exchange is one of these processes.
On a cellular level, Mrs. Jones’ cells are dehydrated due to osmotic pressure changes related to her high blood glucose. Cells dehydrate when poor cellular diffusion of glucose causes increased concentrations of glucose outside of the cell and lesser concentrations inside of the cell. Diffusion refers to the movement of particles from one gradient to another. In simple diffusion there is a stabilization of unequal of particles on either side of a permeable membrane through which the particles move freely to equalize the particles on both sides. The more complex facilitated diffusion is a passive transport of large particles from a high concentration of particles to a lower concentration of particles with the aid of a transport protein (Porth, 2011). The cellular membranes in our bodies are semipermeable allowing for smaller molecules to flow freely from the intracellular to extracellular space. The glucose molecule, however; is too large to diffuse through the cellul...
Membranes play an integral function in trapping and securing metabolic products within the borders of a cell within an aqueous environment. Without a selectively permeable border surrounding sites of anabolic function, potential useful products of this metabolism would simply diffuse away in the aqueous environment contained within and surrounding the cell. However, securing metabolites within the cell also comes with a price of not being able to acquire potentially useful compounds from the surrounding environment. Some very small gases and polar uncharged compounds are able to simply diffuse across this membrane, moving to the site of lower concentration on either side of the membrane. However, larger uncharged and charged polar molecules,
Here, deep in the lungs, oxygen diffuses through the alveoli walls and into the blood in the capillaries and gaseous waste products in the blood—mainly carbon dioxide—diffuse through the capillary walls and into the alveoli. But if something prevents the oxygen from reaching t...
Knife to cut the potato to the correct shape and size for reasons. stated in the method. Tile to not score any school surfaces. Test tubes to hold the potato and solution in. Beaker to alter solution concentration.
However, in order to measure the rates of reaction, sodium thiosulphate and starch are added. Sodium thiosulphate is added to react with a certain amount of iodine as it is made. Without the thiosulphate, the solution would turn blue/black immediately, due to the iodine and starch. The thiosulphate ions allow the rate of reaction to be determined by delaying the reaction so that it is practical to measure the time it takes for the iodine to react with the thiosulphate. After the all the thiosulphate has reacted with the iodine, the free iodine displays a dark blue/black colour with the starch. If t is the time for the blue/black colour to appear, then 1/t is a measure of the initial rate.
Prediction I think that when the potato is placed in distilled water the potato mass will increase. This is because water, has, if pure, a weaker concentration than the potato, and the water molecules move in through a partially permeable membrane by osmosis. If the potato is in a high concentration salt solution, the potato mass will decrease because the potato is less concentrated than the salt solution, and the water will move through the partially permeable membrane into the stronger solution. However, if the potato mass stays the same after the experiment, this means that the water/salt solution and the potato must be of equal concentration.
Moreover, water moves inside and around the cell by osmotic pressure within each compartment and pulls fluid from one area to the other. The level of osmotic pressure remains approximately the same in ICF and ECF. Osmotic pressure can also be defined as the attraction of water to