Process And Ointment: Extraction And Preparation Of Formulations

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Extraction and preparation of formulation:
SOXHLET APPARATUS :
Preparation of the extract :
 The air-dried powdered leaves was defatted with petroleum ether for 24 h followed by extracting with ethanol using a soxhlet apparatus for 18hours. After extraction, the liquid extract was concentrated under vacuum to yield dry extract. The dried residue (extract) was preserved in a desiccator until further use . About 5 g of semisolid extract was incorporated into the 100 g of simple ointment base B.P. Simple ointment base was used as control group. Extract ointment was used once daily to treat different groups of animals.
There are various few other extraction process.

Animals:
Male wistar rats with body weight between 175-225gm were used in these …show more content…

Wound contraction was measured in each 3 days interval, until complete wound healing and expressed in percentage of healed wound area. The epithelization time was measured from initial day.
Measurement of tensile strength:
Tensile strength is the resistance to breaking under tension. It indicates how much the repaired tissue resists to breaking under tension and may indicate in part the quality of repaired tissue. Sutures were removed on the 9th day after wound creation and the tensile strength was measured. For this purpose, the newly formed tissue including scar was excised and tensile strength was measured with the help of tensiometer, which is based on method of Kuwano. In this method wound breaking strength was measured as the weight of water at the time of wound breaking per area of the specimen.
Dry granulation weight:
From the collected tissue 10th day skin sample of dead space wound (granulomas) was excised from cotton pellets and granuloma mass was dried and weighed, while the other part of granuloma tissue was used for determination of tensile

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