Preparation of Ethanol and Ethanoic Acid
Introduction to report
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This report contains 5 practical experiments to produce ethanoic acid
from ethanol. The first practical is the preparation of ethanol from
glucose using yeast during the process of fermentation; this has been
demonstrated in class. In this practical the glucose is converted into
ethanol and carbon dioxide by respiratory enzymes from the yeast. The
ethanol solution will be between 5-15% and the ethanol will be
separated from the yeast by filtering. Then the ethanol will be
divided.
In the second experiment we are going to distillate ethanol solution,
which involve measuring both the volume and the mass of the ethanol
solution, we can work out the density from the volume and mass. We
will then compare the density of the solution with that of pure water
and pure ethanol; it is possible to calculate the percentage
concentration of the solution.
The third practical will be oxidising ethanol to ethanoic acid, in
this experiment we will start with 96% ethanol. We can achieve a
successful oxidation by boiling gently under reflux with acidified
sodium dichromate.
The fourth practical is to distillate ethanoic acid solution; this is
the continuation of the third practical and involves distilling the
mixture to obtain a reasonably pure sample of ethanoic acid.
The final practical is the filtration of ethanoic acid solution; this
involves determining the actual % yield of ethanoic acid by titration
against 0.05 M sodium hydroxide.
Practical one Equation
yeast will carry out anaerobic respiration, using the glucose to
enable it to grow and multiply. The equation above shows what the
yeast will accomplish inside the bioreactor.
This equation also shows fermentation process, which proves an
anaerobic respiration, which means that oxygen is absent from the
process. Anaerobic respiration takes place in organisms and releases a
small amount of energy very quickly. In most organisms, it consists of
a chain of chemical reactions called glycolysis, which break down
glucose into pyrutic acid.
Fermentation is the biological process which allows humans to brew beer, or any other alcoholic beverage. This process occurs in the absence of oxygen, as a means for the cell to produce adenosine triphosphate (ATP), the source of cellular energy. Though little energy can be produced in this manner, it allows the yeast to survive in t...
The purpose of this investigation is to test the effects of multiple sugar substances on the respiration of yeast. Most people think of yeast when they think of what makes bread rise, cheese, alcoholic beverages, or other food products. Another type of yeast can also cause yeast infections, an infection of the skin. Yeasts (Saccharomyces) are tiny, microscopic organisms with a thin membrane and are usually oval or circular-shaped. They are a type of single-celled fungi of the class Ascomycetes, capable of processing sugar into alcohol and carbon dioxide (CO2 ) ; this process is known as fermentation. Fermentation and the products are the main focus points for this experiment being that cellular respiration of yeasts happens via the process of fermentation, which creates by-products of alcohol and CO2. The level of CO2 produced by the yeasts will show how effective each sugar substance is in providing cellular energy for the yeasts.
The fuels are Ethanol, Propanol, Propan-2-ol. Butanol and Butan-2-ol. Setting up the practicals. Because there are some restrictions on the time we are going to have to perform the experiment, we are first going to find out a set up. that would allow us to produce definite results quickly.
The butyl ethanoate ester can only be synthesised during the reflux stage. The ester will continue to be synthesised from the butanol and ethanoic acid until the point where the absence of the limiting reagent prevents further condensation from taking place. Butanol can be considered as the limiting reagent, preventing the total yield of butyl ethanoate which is obtained. Once all of the butanol has been consumed through the reaction with ethanoic acid, the reaction will continue in the reverse direction in an attempt to rejuvenate the supply of butanol. The reverse of condensation is referred to as hydrolysis, a chemical process which produces and alcohol and carboxylic acid when an ester is reacted with water in acidic conditions.
They are as follows, Table #5 substituted glucose for sucrose and Table #6 substituted the glucose for pH4. The equation for alcohol fermentation consists of 6 Carbons, 12 Hydrogens, 6 Oxygen to produce 2 pyruvates plus 2 ATP. Then finally the final reaction will be 2 CO2 plus Ethanol. In the class our controlled numbers were at Table #1.
Organic compounds are commonly found every day in every part of life. Propionaldehyde is a common organic compound also known as propanal, propaldehyde, and methylacetaldehyde with propanal being the most common alternate name. It is a clear colorless liquid at room temperature. Coming from the functional group of aldehyde, propionaldehyde has a C=O bond in replace with two hydrogen atoms, which would be the base propane molecule. The molecular formula is C3H6O, the structural formula CH3CH2CHO, the structure is found in figure 1 (end). Propionaldehyde can only be produced in few ways by a few companies and is used in many various products. There are many few specifically know health effects of propionaldehyde, but it is a very dangerous compound due to its chemical properties.
Aerobic requires oxygen and takes place inside the mitochondria of iving cells. The energy is stored as adenosine triphosphate (ATP) Aerobic respiration produces 2890KJ/Mole or 38ATP. This is much more than anaerobic. The
“Fermentation occurs in fruits, bacteria, yeasts, fungi, as well as in mammalian muscle”(Biology Online, 2008, p. xx-xx) . “Yeasts were discovered to have connection with fermentation as observed by the French chemist, Louis Pasteur” (Biology Online, 2008, p. xx-xx). “Pasteur originally defined fermentation as respiration without air” (Biology Online, 2008, p. xx-xx). “However, fermentation does not have to always occur in anaerobic condition” (Biology Online, 2008, p. xx-xx). “Yeasts still prefer to undergo fermentation to process organic compounds and generate ATP even in the presence of oxygen” (Biology Online, 2008, p. xx-xx). “However, in mammalian muscles, they turn from oxidative phosphorylation (of cellular respiration) to fermentation when oxygen supply becomes limited, especially during a strenuous activity such as intensive exercising” (Biology Online, 2008, p. xx-xx).
A secondary alcohol, such as cyclohexanol, undergoes dehydration by an E1 mechanism. The key intermediate in the mechanism is a cyclohexyl cation, which can undergo substitution as well as elimination. To prepare a cyclohexene (olefin) in good yield, it is necessary to suppress the substitution reaction. In this experiment, the substitution reaction is suppressed by: (1) the use of strong acids with anions that are relatively poor nucleophiles ; (2) a high reaction temperature,
The overall basis of this experiment is to take two compounds, in this case toluene and hexane, distill both and observe a constant boiling point in order to later distill a more complex two component mixture which will provide the pure compound. The distillation process begins with heating a liquid to a boiling point, in this experiment that being hexane and toluene separately to start off, the liquid evaporates forming a vapor. A stir bar was provided in order to ensure that an even boiling of the liquid could happen and therefore produce a place where bubbles of vapor can form. We then recorded the temperatures after collecting different amount of Ml hexane distillate, the same steps repeated for toluene as well. The distillate is a purified
Preparing Benzoic Acid from Benzylalcohol Planning (a) Problem The aim of this experiment is to synthesize benzioc acid, with the highest possible yield, by oxidizing benzylalcohol. Hypothesis We expect the percentage yield to be about 50% due to several processes such as cooling and filtering. Possible Variables - Time - Temperature (of water) - Filter Planning (b) Apparatus/ Materials - Round bottomed flask under reflux - benzylalcohol - HCl - Na2O4 - Büchner funnel - beakers - sodium carbonate Procedure The benzioc acid is synthesized by heating benzylalcohol in a round bottomed flask under reflux. In addition to that, we use Na2O4 as a oxidizing agent. After that, we use HCl to precipitate it.
TutorVista.com (2015), states that; “photosynthesis and cellular respiration are metabolic reactions that complete each other in the environment. They are the same reactions but occur in reverse. In photosynthesis, carbon dioxide and water yield glucose and oxygen respiration, process glucose and oxygen yield carbon dioxide and water, catabolic pathway process which requires or contains molecular oxygen for the production of adenosine triphosphate. This three step aerobic respiration cycle occurs in the cytoplasm and in the organelles called mitochondria. Within this process, cells break down oxygen and glucose in its storable form called adenosine triphosphate or ATP. This cellular respiration or sometimes called an exothermic reaction is similar to a combustion type reaction whereby the cell releases energy in the form heat but at a much slower rate within a living cell. According to our text, Campbell Essential Biology with Physiology, (2010, pg. 94), cellular respiration is stated as “The aerobic harvesting of energy from food molecules; the energy-releasing chemical breakdown of food molecules, such as glucose, and the storage of potential energy in a form that cells can use to perform work; involves glycolysis, the citric acid cycle, the electron transport chain, and chemiosmosis”. It is also my understanding that it is possible for cellular respiration to take place without oxygen, which is called anaerobic respiration. In the anaerobic respiration process the glycosis step or sometimes referred to as the metabolic pathway process deferrers because the anaerobic condition produces
Although not shown in the fermentation reaction, numerous other end products are formed during the course of fermentation Simple Sugar → Ethyl Alcohol + Carbon Dioxide C6 H12 O6 → 2C H3 CH2 OH + 2CO2 The basic respiration reaction is shown below. The differences between an-aerobic fermentation and aerobic respiration can be seen in the end products. Under aerobic conditions, yeasts convert sugars to
Yeasts are facultative anaerobes. They are able to metabolize the sugars in two different ways which is aerobic respiration in the presence of oxygen and anaerobic respiration in the absence of oxygen. The aerobic respiration also known as cellular respiration takes place when glucose is broken down in the present of oxygen to yield carbon dioxide, water and energy in the form of ATP. While in anaerobic respiration, fermentation takes place because it occurs in the absence of external electron acceptor. Because every oxidation has to be coupled to a reduction of compound derived from electron donor. On the other hand, in cellular respiration an exogenous
The process of alcoholic fermentation begins with the use of enzymes. The enzymes begin to break down the long chains in starch molecules, a polysaccharide that consists of a large quantity of glucose molecules (C6H12O6) joined by glycosidic bonds as seen in figure 1, into single glucose molecules, a monosaccharide with six carbons and five hydroxyl groups. After the starch has become sugar, the enzymes are used once again, this time to convert the sugars into ethyl alcohol and carbon dioxide, CO2, as seen in figure 2 (World of Scientific Discovery, 2007). The carbon dioxide produced is released into the atmosphere, leaving water and ethanol, the alcohol, behind. Ethanol is a colorless flammable liquid with a molecular formula of C2H6O, giving it a molar mass of 46.07 grams per mole. Ethanol is also characterized by a melting point of -114°C or 159 K.