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Investigation of the Movement of Pigment through Cell Membranes
Aim
The aim of the investigation is to identify the effect of temperature
of the movement of pigment through cell membrane of beetroot cells.
Prediction and Reasoning
[IMAGE]I predict that the temperature will effect the movement of the
beetroot pigment through the cell membrane. I believe an increase in
temperature would result in an increase in the amount of pigment being
released out of the cell. I base my prediction on the fact that an
increase in temperature would provide the pigment molecules with more
kinetic energy and therefore the rate of diffusion will increase.
The increase in temperature would also denature (damage or change the
characteristics of) the cell membrane resulting in more pigment being
lost. Allowing the pigment to diffuse more rapidly throughout the two
dimensional surface of the membrane.
In the diagram the pigment from the beetroot (section A) would pass
more readily into the water (section B) if it had more energy and also
would pass quicker if the membrane becomes denatured by the increased
kinetic energy of the molecules and the high temperatures.
Equipment
The equipment needed to carry out the experiment to a high standard
includes:
-Beetroot- To investigate the movement of pigment through the membrane
at different temperatures.
-Test Tube Racks- To ensure safety and hold the test tubes.
-Glass Marker- Helps to reduce inaccuracies and misunderstanding with
other specimen test tubes.
-250ml Beaker- To hold the distilled heated water.
-Cork borers- To cut through the beetroot and obtain a 5cm cylinder of
it.
-Thermometer-To accurately measure the temperature of the water.
-Bunsen Burner- To heat the water to 85 ºc.
-Gauze- For the heated beaker to sit on top of.
-Heat proof mat- To stop the table form catching fire.
-Tripod- To apply a safe distance between the beaker and Bunsen burner.
-Colorimeter- To show the concentration of the water once the beetroot
pigment has diffused.
-Ten Test Tubes- To hold each different sample.