Investigating the Concentration of Sucrose in Cells of Carrots and Potatoes
Plan
Introduction
Osmosis is defined as 'the net movement of water molecules from a
region of high water concentration to a region of lower water
concentration through a partially permeable membrane'. In my
experiment the process of osmosis will be due to the concentration of
sucrose inside the cell compared to outside. Therefore if there is a
higher concentration of sucrose molecules outside the cell then the
net movement of water molecules will be out of the cell and similarly
if there is a higher concentration of sucrose molecules inside the
cell then the net movement of water molecules will be into the cell.
This movement of water molecules causes the cells to become either
turgid, which is when the cells are filled with water, or flaccid,
which is when the cells are 'limp' with little water.
Aim
To find the concentration of sucrose in cells of carrot and potato.
Osmosis
When a substance such as sugar dissolves in water, the sugar molecules
attract some of the water molecules and they stick to the sugar
molecules. This means that there are fewer free water molecules in the
solution. When two different sugar solutions come into contact with
each other through a partially permeable membrane the free molecules
can go through the membrane easily but the sugar molecules are too
big. If one of the solutions has more free molecules than the other
then more of those free molecules will pass through the membrane than
the other way round, hence the definition. The following diagram
illustrates this
Low Water High Water
Concentration Concentration
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Investigation of the Concentration and the Effect of Sucrose on Osmosis in Apple and Potato Tissues
In separate test tubes 6. Cut each celery piece into 5 cm cubes and place into test tubes 7. Leave for 30 mins at room temperature 8. And collect the strips out of the test tubes, dry them and record the mass of each strip Prediction I predict that as the concentration of sucrose increases, The cell will firstly become turgid, as since the concentration is low the water potential outside the cell will be higher than inside to water will diffuse in. Then as the concentration gets higher the water potential outside will become lower than inside the cell so water will diffuse out and the cell will become plasmolysed.
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
I blended on high to make the potatoes more liquid-like. I grabbed the cheesecloth and placed on the top of the blender. I poured the potato extract on the container and labeled it. I found out that I have to make 1% sugar solution so I grabbed the sugar and measured into 5 grams on the scale. I added 5 grams of sugar on 250 ml graduated cylinder and poured the water into the cylinder. I mixed the sugar with water and poured it into the saucepan. I refilled the water into the graduated cylinder and poured into the saucepan. I turned on the heat of the stove and saw the sugar dissolved. I poured into a container and labeled 1% sugar solution. I repeated the same thing with 1% salt solution by using 1 gram of salt and filled the water into graduated cylinder by 100 ml. I answered question three. In the first experiment, I grabbed four transfer pipets and used it to put solutions into the test tubes by 3ml. I labeled it and placed into the plastic cups so it can stand upright. I grabbed each test tube and poured 2 ml of catalase solution into it. I also tapped and swirled to measure the bubbles by using the ruler. I wrote the numbers into the lab report. In the second experiment, I labeled the room
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
Osmosis in Carrots Background Osmosis is the diffusion of water from a dilute solution to a more concentrated solution through a partially permeable membrane, which allows the pass of water molecules but not solute molecules. [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE]If a cell is placed in a less concentrated solution water enters because the less concentrated solution will have a high concentration of water than the inside of the cell. Once the cell takes in maximum water the cell becomes turgid. If the cell was to be placed in a high concentrated solution, water would leave the cell because the cell would contain a low concentrated solution. So in the low concentrated solution there will be a high concentration of water and in the high concentrated solution there will be a low concentration of water.
Investigate the Osmosis of Potato Cells in Various Salt Solutions. Introduction I have been asked to investigate the effect of changing the concentration of a solution on the movement of water into and out of potato cells. I will be able to change the input of my experiment. The input variable is the concentration of the solution.
cork borer and a ruler. I will keep the potato chips the same size in
To investigate the osmotic effect of changing the concentration of sucrose solution; distilled water, 20% sucrose solution, 40% sucrose solution, 60% sucrose solution on the change in mass of potato cylinder after 30 minutes of being in solution.
At point A the graph shows that no change in mass, of the potato, would have have occurred had we used a 0.2 (m) sucrose solution. This suggests that the concentration of water inside the potato would have been equal to the solution outside the potato. At point B (plain water), there is no indication that the cell is increasing in mass. This is because the cell is fully turgid and no more water can enter.
repeat the process 3 times in total to ensure a fair test. At the end
The Effect of Solute Concentration on the Rate of Osmosis Aim: To test and observe how the concentration gradient between a potato and water & sugar solution will affect the rate of osmosis. Introduction: Osmosis is defined as, diffusion, or net movement, of free water molecules from high to low concentration through a semi-permeable membrane. When a substance, such as sugar (which we will be using in the experiment we are about to analyse), dissolves in water, it attracts free water molecules to itself, and in doing so, stops them from moving freely. The effect of this, is that the concentration of (free) water molecules in that environment goes down. There are less free water molecules, and therefore less water molecules to pass across a semi-permeable membrane, through which sugar molecules and other molecules attached to them are too big to diffuse across with ease.
the gain or loss of water when samples of the tissue are placed in a
== = This experiment is based on the concept of Osmosis. Osmosis is the diffusion of water molecules from a region of high water concentration to a low water concentration through a semi permeable membrane (in this case, the cell potato cell membrane). The cell walls of the potato cells are semi permeable meaning that water molecules (which are small) can fit through but other bigger molecules such as glucose cannot pass through. The water molecules can flow both ways through the membrane, letting molecules both in and out.