General Protocol Lab Report

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32. General Protocol :Now repeat steps 17-28 for temperatures: 0°C, 10°C, 20°C, 30°C, 40°C, 50°C, and 60°C, testing three times each. Always put the catalase into the ice chest when not in use. Repeat with new hydrogen peroxide every time and make sure the temperature of the hydrogen peroxide is the temperature you are testing. Clean out jars immediately after testing and refill them with hydrogen peroxide, so multiple temperatures can be tested at once. There are 2 hours to test 20 runs (not including the 1st room temperature test) before the catalase starts denaturing (breaking down).
33. For temperatures 0°C and 10°C, use the jars in the fridge and the freezer. Repeat the process of putting jars with hydrogen peroxide in the freezer for 10 minutes and the fridge for 20 minutes, and then follow general protocol until you have tested each temperature three times.
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To test room temperature/20°C, just leave out hydrogen peroxide filled jars with no added or lessened heat.
35. To test 30°C, fill a cooking pot with water to a depth of about 4 cm. Heat the pot of water on a stove until the water just barely boils. Then immediately take the pot off the burner to let it cool. Take the water’s temperature. When the water reaches approximately 40°C, carefully put the jar with the hydrogen peroxide into the pot of water. Remove some water from the pot if the water level is so high that water might flow into the jar or if the jar floats, even a little. When the hydrogen peroxide reaches 30°C, follow the general

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