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Investigation into the respiration of yeast
Investigation into yeast respiration
Investigation into yeast respiration
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Recommended: Investigation into the respiration of yeast
Aim:
To see which type of lighting shatters yeast cells at a faster rate
Hypothesis:
The brightly coloured lights will affect the yeast cells at a faster rate. This is because brightly coloured lights make its strength weaker and darker lights maintain its strength.
Background research:
Yeast
“Defined as a chemoorganotrophs, which is an organism that requires an organic source of carbon and metabolic energy.” (Medical Dictionary, 2012)
Several experiments have been conducted on how light affects yeast. Most results have been clearly presented the same. Tennessee State University studied changes in yeast respiration in the presence of blue and green light, find that light can be used to control short period respiratory oscillations in
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Often this experiment is positioned in a dark room, so light does not separate the cell membranes and shatter them. Therefore different colours of light destroy the cells at different rates. “A cell is referred to a thin layer of tissue that covers a surface, divides a space, line a cavity or an organ. A cell membrane allows certain substances to pass through enclosing the cytoplasm of a cell.” (Medical Dictionary, 2012)
Yeast is extremely sensitised. Yeast that comes in contact with too hot or too cold water is fatal to the yeast. Water that is between 130-140˚F is lethal to yeast. For activation of dry yeast water should be between 105˚c-110 degrees Celsius for proofing. 95 degrees Celsius is the recommended temperature according to Cookistry for yeast to
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Retrieve yeast and stir into corresponding bowl of water.
3. Time how long till yeast mixture bubbles. For undamaged yeast, should take about ten minutes. Take a picture at ten minutes, fifteen minutes and twenty minutes.
4. Repeat step three for all yeast samples.
5. Analyse the data.
Variables:
Variable Type of Variable How is it changed, measured or kept the same
Type of Light Independent Using three types of light bulbs (one red, blue and green) to active the yeast
Time taken for the yeast mixture to bubble Dependent Time with an electronic timer how long each mixture takes to bubble.
Amount of water Controlled Measured in a measuring cup, keeping the same amount of liquid each time.
Air temperature Controlled The same air temperature within the room. Checked with two different thermometers.
Amount of yeast Controlled Measured in grams. Checked with a scale.
Type of cardboard box Controlled Three boxes were the same type and size.
Type of metal bowl Controlled Five metal bowls same type and size.
Risk assessment:
Hazards that can
3. The time taken for the yeast to heat up to the temperature of the
Ryazantseva, I. N., Saakov, V. S., Andreyeva, I. N., Ogorodnikova, T. I. and Zuev, Y. F. 2012. Response of pigmented Serratia marcescens to the illumination. Journal of Photochemistry and Photobiology B: Biology, 106 p. 18-23
The results shown in table 1 clearly show that when the volume of yeast is increased in the milk solution, so does the rate of oxygen depletion and therefore the rate of eutrophication. It shows that when 2mL of yeast solution was added it took 32.86 minutes on average for the milk to be depleted of oxygen, while it took only 7.46 minutes on average for the 10mL of yeast to use up the oxygen present.
Increasing the light intensity will make photosynthesis faster. Variables: In this experiment there are a few things we have to keep the same.
I also predict that as the light is moved closer to the plant there will be more bubbles (oxygen) produced due to the increase of photosynthesis speed explained above. So in conclusion I predict that the more light intensity there is on the plant the faster the rate of photosynthesis there will be.
Similar to how we used water on both sides as a control in the baseline experiments, our first test was with dim light on both sides. To set up the chamber, we put 52 fruit flies in the double-sided chamber and sealed it tightly. Make certain to get at least 20 flies in the experiment for a larger set of data. That is why we put 52 flies in the choice chamber- to get substantial data. In order to count the number of flies on each side, draw a line going down the middle of the choice chamber to divide it into two halves. Side A is one half, and Side B is the other half. In this lab, we used red, blue, black, green, and 2 white light bulbs throughout the experiment. Additionally, to measure the intensity of the light hitting the choice chamber,
Experiment #1: The purpose of this experiment is to investigate the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leave through the observation of floating disk.
· Add 2g of yeast to the water and add sugar (1g, 2g, …up to 5g).
= > [CH2O} + O2 + H2O, This shows that when the light intensity is increased the rate of reaction will be more quicker he only anomalous result there was, is the one in the 100 watt result the reading after 5 minutes is anomalous because it does not follow the predicted pattern of increasing in the production of gas because it is lower I know from my own knowledge of photosynthesise that when the light intensity is increased the rate of reaction will be more quicker because many plants and trees photosynthesise quicker in stronger light and photosynthesise slower in dimly lit places. The chlorophyll absorbs light energy and enables it to be used by the plant for building up sugar. The overall effect is that energy is transferred from sunlight to sugar molecules.
The Effects of Concentration of Sugar on the Respiration Rate of Yeast Investigating the effect of concentration of sugar on the respiration rate of yeast We did an investigation to find how different concentrations of sugar effect the respiration rate of yeast and which type of concentration works best. Respiration is not breathing in and out; it is the breakdown of glucose to make energy using oxygen. Every living cell in every living organism uses respiration to make energy all the time. Plants respire (as well as photosynthesise) to release energy for growth, active uptake, etc…. They can also respire anaerobically (without oxygen) to produce ethanol and carbon dioxide as by-products.
The purpose of this investigation is to test the effects of multiple sugar substances on the respiration of yeast. Most people think of yeast when they think of what makes bread rise, cheese, alcoholic beverages, or other food products. Another type of yeast can also cause yeast infections, an infection of the skin. Yeasts (Saccharomyces) are tiny, microscopic organisms with a thin membrane and are usually oval or circular-shaped. They are a type of single-celled fungi of the class Ascomycetes, capable of processing sugar into alcohol and carbon dioxide (CO2 ) ; this process is known as fermentation. Fermentation and the products are the main focus points for this experiment being that cellular respiration of yeasts happens via the process of fermentation, which creates by-products of alcohol and CO2. The level of CO2 produced by the yeasts will show how effective each sugar substance is in providing cellular energy for the yeasts.
There were five test solutions used in this experiment, water being the control, which were mixed with a yeast solution to cause fermentation. A 1ml pipetman was used to measure 1 ml of each of the test solutions and placed them in separated test tubes. The 1 ml pipetman was then used to take 1ml of the yeast solution, and placed 1ml of yeast into the five test tubes all containing 1 ml of the test solutions. A 1ml graduated pipette was placed separately in each of the test tubes and extracted 1ml of the solutions into it. Once the mixture was in the pipette, someone from the group placed a piece of parafilm securely on the open end of the pipette and upon completion removed the top part of the graduated pipette.
This means that there should be more growth, or cell division. This allows for me to determine the mitotic index of the bulbs. The mitotic index is a ratio of cells in a sample that are in a phase of mitosis (prophase, metaphase, anaphase, telophase, or cytokinesis) to the total amount of cells in a sample. As light intensity is a factor that affects cell growth, the mitotic index should be
The reason light intensity is being used compared to whether or not a plant needs light. It is because The experiment wants to show that the rates of photosynthesis will vary according to how much light from a light bulb will be trapped in. the chloroplasts, in the leaf. The more energy trapped the more efficient a chemical reaction can take place and the speed of photosynthesis will increase. There are many things which can affect the photosynthesis of a plant such as light intensity, temperature and carbon dioxide levels.
The objective of our project was to determine if different wavelengths of light would affect the growth of the Rhodococcus genus and whether the two bacteria would handle DNA damage from UV light exposure differently. Our hypothesis is that various wavelengths of light would inhibit the growth of bacterial colonies of both Rhodococcus species differently. Also, we believe that Rhodococcus fascians (yellow) is more resistant to DNA damage from UV light than Rhodococcus corynebacterioides (red). To perform this experiment, we first made the bacterial growth medium was made. Then, the process of the dilution of the bacteria is performed to allow the bacteria to be seen easily. The diluted bacteria is spread upon the medium in the petri dishes