Forensic Chemistry: Body Decomposition and Estimation of Post-Mortem Interval

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When a person's body decomposes after death, it goes through a series of stages involving different chemical changes that cause it to change its appareance. Observing these changes can help forensic scientists determine the post-mortem interval (or time since death), which is very important when it comes to investigating unnatural deaths.

The stages of decomposition are: fresh stage, bloat stage, active decay, advanced decay, and dry stage. [1]

The fresh stage begins right after the heart stops beating.
When the blood stops being pumped, gravity makes it settle and it changes the color of the skin where it pools, making it purple-red. This is known as livor mortis or hypostasis. [2]
Then, rigor mortis occurs. Rigor mortis is the stiffening of the muscles due to the disappareance of ATP (adenosin triphosphate). The proteins responsible for muscle contraction, actin and myosin, need ATP to create crossbridges and make the muscles contract, and then relax. When ATP is no longer produced by the cells, the cycle of contraction cannot be completed and the muscles remain contracted. [3]
Since the moment of death, the body starts losing heat to the surroundings until it reaches ambient temperature. This process is called algor mortis.
The chemical changes happening in the body affect the pH, which makes the cells release enzymes that break down the surrounding tissues in a process called autolysis. In this stage of decomposition autolysis does not cause many visible changes, other than blistering of the skin.
When the aerobic organisms in the body consume all the oxygen present, anaerobic organisms from the digestive system begin to multiply. They consume macromolecules (proteins, carbohydrates, lipids) and form acids and gases in the p...

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8. Mark, T., & Carter, D.O. (2010). Cadaver Decompossition and Soil: Processes. Soil Analysis in Forensic Taphonomy: Chemical and Biological Effects of Buried Human Remains (p. 35-36). CRC Press. Retrieved from http://books.google.es/books?id=aksRkfr1d6kC
9. Donaldson, A.E., & Lamont, I.L. (2013). Biochemistry Changes That Occur after Death: Potential Markers for Determining Post-Mortem Interval . PLOS ONE, . Retrieved , from http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0082011 doi:10.1371/journal.pone.0082011
10. Sampaio-Silva, F., Magalhães, T., Carvalho, F., Dinis-Oliveira, R.J., & Silvestre, R. (2013). Profiling of RNA Degradation for Estimation of Post Morterm Interval . PLOS ONE, . Retrieved , from http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0056507 doi:10.1371/journal.pone.0056507

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