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Observations in an osmosis experiment
Observations in an osmosis experiment
Observations in an osmosis experiment
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Conclusion
(1) In the osmosis lab I made a few predictions (a) I predicted that the egg in the hypotonic solution will swell & gain mass because, there are more h20 molecules and less solutes in the distilled water then inside the egg therefore the h20 will go into the egg. (b) I predict that the egg in the hypertonic solution will shrink and lose mass because, there are more salt molecule (solution) and less water molecules in the salt than inside the egg therefore the h20 will the egg. (c) I predict that the egg in the isotonic solution will stay the same size & mass because, there will be the same amount of solutes & H2O inside and outside the egg therefore the H2O will go inside & outside the egg. (2) My hypothesis was not supported by
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the results. When the egg was placed in the hypotonic solution the egg was supposed to gain mass but it infact shrunk. It had been placed in distilled water, in which distilled water is supposed to cause the egg to swell. Making the starting mass 86 g's and the ending mass 83g’s. Therefore the difference in mass was 3.4%. It also didn’t apply to the hypertonic solution. This solution was supposed to shrink because the egg was placed in a salt solution (the hypertonic). Salt is a grain that basically absorbs the water drying the egg out causing all the mass to be lost. In other words, when I put the egg in the hypertonic solution the egg didn't shrink it in fact gained weight. Making the starting weight 89.5g’s and the end weight 97 g. Therefore the difference in mass was 8.3%. The third solution was an isotonic solution which my prediction states that it wasn't supposed to change in weight. Making my starting mass 89 g's and my ending mass 96.5g’s. This solution isn't supposed to change in mass because the amount of water and the amount solutes were supposed to be perfectly balanced out cause the eggs change in mass to be 0% but My eggs difference in mass was 8.4%. (3) In this experiment my group and I could improve on doing more research and making measurements for each experiment to make perfect solution. Then it would have helped if I could have been there more to help and make sure everything was measured correctly. (4) During osmosis a blood cell can either have a hypertonic, hypotonic, or an isotonic solution. your blood cell is what tells you whether you're dehydrated or hydrated.
So when you drink a lot of water and don't balence it with something salty then you could be potentially over hydrated. with that dehydration is when someone doesn't have enough water and can be very unhealthy. By being dehydrated it can cause muscle cramps and fainting. The egg could also be perfectly balanced with a perfect amount of solutes and h2o in the solution to cause to the egg to not change weight. The egg in the lab is doing the same thing our blood cells do everyday. The egg in the osmosis lab is doing the same actions because the shell around the egg is a form of a cell membrane a stretchy substanse like elastic that can expand to a certain point. With this substance the egg can expand and shrink to whatever point its capable of. When the egg was in the hypertonic solution, it was supposed to shrink, but it had gained mass instead. By that I mean the balance was off there was too much water inside the egg then outside. If there was more water outside the egg, the egg would have shrunk a lot. But water makes the egg expand and gain mass. Therefore In the hypotonic solution the egg was supposed to gain in mass, but instead it decreased in mass. The egg mass didn't have a drastic change, but it still was
supposed gain weight. With that said the egg had less water inside than outside like the hypertonic was supposed to. So why did the egg does that. well the hyper and hypo solutions are just solutions with very specific requirements, they both require water but don't require too much water. The lab would have been better if the solutions were measured correctly. In the isotonic solution the eggs balance was completely off there was way too much water in the solution. The egg was over hydrated it had gained way too much weight. The isotonic solution is what it was supposed to be completely perfect it wasn't supposed to gain or lose weight. (5) Unfortunately I would change all solutions not just one, because all of my solutions were wrong. With the solutions I would change the h20 amount. For example the hypertonic solution has to much water in it causing the egg to swell way to much and making it more of a hypotonic solution. If the water and salt were measured correctly then the osmosis would be properly tested, the salt water (hypertonic) solution would shrink the egg like it was supposed to do. With the other eggs and solutions we need to make sure their the right solution for the right cup and egg.
Step 3: Q5. The salt that was placed on the egg turned into ‘sweat’ like beads on the egg. Q6. There is a higher water concentration inside the egg, causing the water to travel through the egg to the salt. The salt creates osmotic pressure on the water molecules in the egg.
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
Background info: hypertonic solution was used in the lab, hypertonic is the liquid substance that is going out of the egg. When a red blood cell has more water than what's outside then the water will come out and will become smaller. Hypotonic is when the water on the outside of the cell has more water than the inside, the water will go in the inside to make it run smoothly. Isotonic is when the inside and outside of the cell is each, they will stay the same.
The purpose of this lab was to show us what will happen when we soak the egg in the tap water overnight. When we soaked the egg in the tap water overnight, the egg appeared to be swollen. The egg also gained mass because the egg has less water concentration.
During the first day, we weighted the egg and put the vinegar in the cup. Then, we let the vinegar sit on the egg for 24 hours. The next day ,day 2, we spilt out the vinegar ,and added distilled water. We also weighted the mass of the egg ,and described the appearance of the egg on the data chart.
Place one egg in a beaker. Fill this beaker with distilled water to just cover the egg. See Figure 1. Note the appearance of the water at this time and record your observation in Data Table 3. CAUTION: Be careful to avoid breaking glassware.
It was stated in the hypothesis that, “If cucumber slices are placed in water, ten percent sugar solution, and twenty percent water solution, then the cucumber in water will be isotonic (have the same measurement), the cucumber in the ten percent sugar water will become hypertonic (have smaller measurement), and the cucumber in the twenty percent sugar solution will be very hypertonic (even smaller). This is because of the process of osmosis and how things travel on the concentration gradient.” To test the hypothesis, three cucumbers were placed in three different solutions after being tested for their volume and mass. One solution held only water, another had 10% sugar, and the last had 20% sugar. After letting the cucumbers soak in each solution, their volume and mass was tested again. As shown in the table, the solution with only water had made the cucumber hypotonic. The volume was increased .16 and the mass increased .36g. In the 10% and 20% sugar solutions, the cucumber became hypertonic. The volume and mass of each cucumber had decreased. In the 10% solution, the volume went down by .16 and the mass went down .47g. In the 20% sugar solution, the volume decreased by .10 and the mass by .9g. The data collected from the lab proved our hypothesis was correct. One way to improve this lab would be to make sure the cucumbers were all the exact same size. This would make the data more accurate and easier to
In the diffusion and osmosis lab, we were to prove if there was a change in the weight of a potato sample once placed in a sucrose solution. Our hypothesis was proven false, due to the sufficient amount of sucrose, the weight of the object decreases. The Enzyme experiment consisted of two parts, the first including the pH concentration, we were off to reveal if a high concentration of pH could increase its reaction rate. We tested our hypothesis, and it was incorrect, the closer the pHs were to neutral the faster the starch disappeared. The second including different temperatures on the Amylase enzyme.
Diffusion and osmosis in living cells are greatly effected by factors such as solution concentration, temperature, and surface area of the cell. This was studied using agar cubes of different sizes, dialysis tubing, and potato cores to demonstrate how the different factors affect cells. The process of cellular respiration can be altered due to temperature differences. Enzymes also greatly affect the molecular processes that occur. Enzymes are denatured at variety of pH and causes inactivity of the enzyme in the reactions.
The data in both Table 2 and Graph 1 show that as the concentration of sodium chloride (%) is increased from 0% to 10% when there is a significant increase in the % change in mass, thus also the rate of osmosis. Between concentrations 0% and 2% there was a rapid % change in mass. At 0% NaCl the % change in mass was +1.63% indicating that the potato piece had gained water because the concentration of solute must have been higher in the potato than in the solution. The % change in mass at 2% NaCl concentration was -8.82%, the negative indicates that the potato piece had lost water as the concentration of solute was higher in the solution than the potato piece. From 2% to 4% NaCl solution the %change in mass was slightly less rapid but still had an increase from -8.82 to -14.7, respectively. The graph had a slower decreasing trend from 4% NaCl solution onwards, this can be seen from the gradual plateau of the graph. The slowest increase in %change in mass of -0.04% was between 6% to 8% valued at -16.5% and -16.9%, respectively. The % change in mass increased slightly from -16.9% to -17.5% between 8% to 10% NaCl concentration. This suggests that the rate of osmosis had begun to stabilize and may be the result of the concentrations of both the
Determining the Concentration of the Cell Sap in Potato Storage Tissue Aim: To determine the concentration of the cell sap in potato storage tissue. By using Osmosis, determine what the sugar concentration of cell sap is. Prediction I predict that the potato segment in the distilled water will definitely gain in weight because the solution outside it has a much higher concentration of water then in the cell sap meaning Osmosis will occur and the potato segment take in water. I predict that 0.2M sugar solution will also gain weight because it still has quite a high concentration of water outside the potato. The potato in the 0.4M solution will gain weight but the potato in the 0.6M and 0.8M solutions will lose weight.
Introduction: During the Osmosis activity we were expected to complete an experiment to determine the impact of temperature and concentration on osmosis. To determine the effect of temperature on osmosis we were require to test three dialysis bags filled with syrup. Each bag was first measured for initial weight, then assigned a temperature at which that bag will be tested in. We filled three beakers with the same amount of water, but at different temperatures.
Osmosis and Diffusion are two important key concepts that helps the cell as a whole functional. Diffusion is simply the movement of a substance from an area that has higher concentration to an area that has a smaller amount of concentration. Osmosis is the total opposite of Diffusion; Osmosis consists of a solvent being able to pass freely through the membrane of a less concentrated solution to a higher concentrated solution.
Osmosis and diffusion are two methods which work towards levelling the concentration in the two given surfaces – the cell and the environment. Purchon defines osmosis as the movement of water from a higher concentration to a lower concentration “through a semi permeable membrane”. For instance; a red blood cell when placed in a hypertonic solution undergoes crenation as water from cell moves down the concentration gradient into the environment. Osmosis is only for water molecules while the similar movements of other particles down their concentration gradient is known as diffusion. Diffusion can happen in various mediums such as liquid and air. For example, the cologne particles when released, diffuses through the air. It is necessary to understand the purpose of osmosis and diffusion to get an idea about the importance of homeostasis in our body and how the organs systems work towards
In this experiment the Sodium Hydroxide solution went through three different phases where its quality and quantity changed. The first phase was called I. Preparing Approximately 0.1M NaOH, 1000mL of clear distilled water was boiled and then chilled to room temp.