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The effects of osmosis on cells and tissues
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Eggmosis
Purpose
The purpose of this lab was to investigate osmosis effects on a real cell.
Hypothesis
If the egg is placed into corn syrup, then it will shrink.
If the egg is placed into distilled water, then it will swell.
Background Information
This lab demonstrates one type of molecular movement, passive transports, displays the effects solutions have on a cell, a chemical reaction, and how the cell membrane works.
Passive transports do not require any energy because the molecules move from an area of high concentration to low. Diffusion, a type of passive transport, is the net movement of the same kind of molecule that pass through the phospholipid bilayer. Osmosis, a type of diffusion, is the diffusion of specifically water through a selectively permeable membrane and as the concentration of water in a solution increases, the concentration of solutes will decrease.
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There are three types of solutions that are used in this lab.
Isotonic solutions are when the solute concentration outside of the cell is the same as it is on the inside of the cell. Water moves both in and out of the cell, there is no net water movement, and this solution has no effect on the cell. Hypertonic solutions have a higher solute concentration outside of the cell than inside, will make a cell shrink, and the net movement of water is water moving out of the cell. Hypotonic solutions are the opposite of hypertonic solutions. This solution has a higher solute concentration on the outside of the cell than the inside, the net movement of water is into the cell, and causes cells to swell and possibly
burst. Vinegar is an acetic acid and when chemically combined with calcium carbonate; it creates carbon dioxide. The cell membrane is a selectively permeable boundary, meaning it controls what enters and exits the cell. The cell membrane is made up of a phospholipid bilayer. This bilayer is composed of hydrophilic heads with two hydrophobic tails, cholesterol, transport proteins, and carbohydrate chains. These water-attracting heads, water-avoiding tails, and transport proteins control how molecules enter and leave the cell. Cholesterol helps to maintain the membrane flexibility and makes the membrane less permeable. Carbohydrate chains allow cells to recognize each other. Materials Egg 500 ml beaker Vinegar Corn syrup Distilled water Digital balance scale Pencil Paper Procedure Day 1 Obtain an egg. Use the digital balance scale to measure the egg’s mass. Record the mass. Place egg in beaker. Fill beaker with vinegar enough to cover egg. Day 2 Record observations. Gently remove egg from beaker. Dispose of vinegar in beaker. Place egg into beaker. Repeat Step 5. Repeat Step 6. Day 3 Hypothesize the effect of caro syrup on the egg. Hypothesize the effect of distilled water on the egg. Remove the egg from the beaker. Rinse out the beaker until vinegar is gone. Record mass of egg. Place egg into beaker. Cover egg with corn syrup until it floats. Record observations. Day 4 Remove egg from beaker. Rinse out beaker. Record the mass of egg. Record observations. Place egg into beaker. Fill beaker with distilled water until egg is covered. Day 5 Remove egg from beaker. Dispose of distilled water. Wash beaker. Record mass of egg. Make observations. Dispose of egg. Data & Results Egg Treatment Weights Equation ~ Final-InitialInitial 100 Treatment Mass (grams) % Change Original Egg 59.21 grams Vinegar 86.36 grams 45.85% Caro Syrup 40.11 grams -53.55% Distilled Water 96.18 grams 139.79% Table 1: Mass and percent of change after each treatment. Conclusion The purpose of this lab was to investigate the effects of osmosis on a cell. The hypotheses made stating that, “ if the egg is placed into corn syrup, then it will shrink” and, “if the egg is placed into distilled water, then it will swell” were accepted.
Specifically for the lab discussed we were to determine whether sucrose of RO H20 was of higher or lower concentration. We were able to determine permeable membranes were depending on the concentration of the solution or solvent. In this case we learned that solution and solvents of lower concentration allow for greater flow through the semi-permeable membrane, which supports the idea of diffusion. We learned that sucrose solution allowed for greater diffusion than RO H20. Also chloride ions and aluminum allowed for diffusion whereas starch and sulfate ions did not. We also learned Elodea in NaCl solution underwent a process called plasmolysis where the cells
Osmosis is a continuous process which does not require any energy to take place. Factors Which Affect the Rate of Osmosis 1) Temperature: The higher the temperature is the faster the molecules will move. This means that the movement of water molecules across the semi permeable membrane will be faster. 2) Surface Area: When there is a larger surface area there will be
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
We are learning about the hypertonic, hypotonic, and isotonic solution in class. So for better understanding of this we are doing an experiment with gummy bears and look what happen if we put them in different solutions. Hypertonic- A hypertonic solution is a particular type of solution that has a greater concentration of solutes on the outside of a cell when compared with the inside of a cell. Hypotonic- solution that has less solute and more water than another solution. Isotonic- In this solution the free movement of water across the membrane without changing the concentration of solutes on either side.
Active transport requires the use of energy because substances are moved against/up a concentration gradient or across a partially permeable membrane. On the other hand, passive transport moves molecules down the concentration gradient and does not require cellular energy. For example, osmosis which is the movement of water across the membrane would be considered a passive transport because the molecules, or in this case water move easy and freely.
In this lab we tested osmosis through a semi permeable membrane using dialysis bags (SCC Science Division, 2014). We also looked at osmosis in living cells of potatoes. When water is moved through a semi permeable membrane that is called osmosis. Passive transport is when molecules move with the concentration gradient across the membrane with no cellular energy used. Osmosis and diffusion are examples of passive transport. There is also active transport which is when molecules move against the concentration gradient and uses energy in the form of ATP (Urry, Cain, Wasserman, Minorsky, Jackson, & Reece, 2014). There are three types of conditions that deal with osmosis that will be found in this experiment: isotonic, hypertonic, and hypotonic solutions. Isotonic is when the concentration of a solute is identical outside of the cell and inside of the cell. Hypertonic is when the concentration of solute is higher outside of the cell than inside of the cell. Hypotonic is when the concentration of a solute is less than the solutions outside the c...
Activity 3: Investigating Osmosis and Diffusion Through Nonliving Membranes. In this activity, through the use of dialysis sacs and varying concentrations of solutions, the movement of water and solutes will be observed through a semipermeable membrane. The gradients at which the solutes NaCl and glucose diffuse is unproportional to any other molecule, therefore they will proceed down their own gradients. However, the same is not true for water, whose concentration gradient is affected by solute ...
When the egg is placed in vinegar, it will be hypotonic. When it's placed in water, it will become hypertonic. When placed in syrup, it will become hypotonic and shrink. In this experiment, we used an egg to see what happens to it during osmosis and diffusion across membranes. Throughout this week, we collected data on what happened to the egg and the mass of the egg.
The purpose of this lab was to see firsthand the diffusion of a substance across a selectively permeable membrane. Diffusion is the movement of molecules from an area of high concentration to an area of lower concentration until both concentrations are equal, or as you could more professionally call it, equilibrium. This concept is one that we have been studying in depth currently in Biology class.
On a cellular level, Mrs. Jones’ cells are dehydrated due to osmotic pressure changes related to her high blood glucose. Cells dehydrate when poor cellular diffusion of glucose causes increased concentrations of glucose outside of the cell and lesser concentrations inside of the cell. Diffusion refers to the movement of particles from one gradient to another. In simple diffusion there is a stabilization of unequal of particles on either side of a permeable membrane through which the particles move freely to equalize the particles on both sides. The more complex facilitated diffusion is a passive transport of large particles from a high concentration of particles to a lower concentration of particles with the aid of a transport protein (Porth, 2011). The cellular membranes in our bodies are semipermeable allowing for smaller molecules to flow freely from the intracellular to extracellular space. The glucose molecule, however; is too large to diffuse through the cellul...
What is a passive transport? A passive transport is the movement of something across the membrane with no energy. There are 4 main passive transports. There is diffusion, osmosis, Facilitated Diffusion, and Filtration. Diffusion were molecules will spread out evenly into empty space. Osmosis is the movement of water across a membrane. Passive transport happens when you get Pruned Fingers. Cholera is also made by osmosis. Cholera is found in our intestines and it reverses the intestinal cells.
One mechanism utilized for passive transport is diffusion. When the concentration of particles is higher in area the particles diffuse to an area of lower concentration from the constant motion of particles. The rate of diffusion can increase when the temperature of the solution is increased because this causes the motion of molecules to increase. Another mechanism utilized in passive transport is osmosis. Osmosis occurs when water moves to an area of higher concentration to equalize the concentration between the cell membranes. Osmotic pressure is the power a solution has to draw water across the membrane. The higher concentration has more osmotic pressure and draws water across the membrane. In the extracellular compartment, oncotic pressure pulls water from the interstitial compartment to the intravascular compartment to equalize the concentrations and maintain volume in the vascular system. Lastly, filtration is utilized to move fluid and particles together across the membrane. Filtration takes place from a higher pressure to lower pressure. Hydrostatic pressure is the pressure a fluid uses to force fluid from greater pressure to an area of less
Every living organism’s salt content is controlled by osmosis. Through osmosis, water moves across an organism’s surface and/or membrane. Osmosis usually moves from high concentration to low concentration. In this experiment we decided to add 300mL of water in 2 jars labeled a Jar A was filled with freshwater while Jar B was created into a saltwater solution. After we added a 1 piece of each of our vegetables which were (carrot, celery and raisin). After we added the vegetables we added cling wrap to each of our jars so the water doesn't evaporate and turn into water vapor. In groups we had to figure out what happens to the vegetables before and after 24 hours in the saltwater, and the freshwater. Vegetables such as carrots
Soluble particles like oxygen are diffused whereas waste products like water and carbon dioxide are released out of the cell using passive transport. Simple diffusion, osmosis and facilitated diffusion are some of the types of passive transport. Diffusion is the passive movement of molecules from a level of higher concentration to lower concentration through a permeable membrane to equalize the concentration. Once the particles have equal concentration on both sides of the cell membrane, they will continue to move in both directions but at an equal rate. For example, water moving in and out of the cell at the same time.
To further understand these mechanisms, five experiments were conducted. These experiments were conducted over simple diffusion, facilitated diffusion, osmotic pressure, simulating filtration, and active transport. These studies were obtained by understanding the changes and observing the different variables of how they affect transport through the membrane. Materials and Methods Simple Diffusion The rate of diffusion affects the size of the molecule and the plasma membrane.