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Recommended: Conausion of osmosis
Jully Garcia
Diffusion and Osmosis
Lab Report
Introduction
We did this experiment to find out the percent of starch concentrations in two different types of solutions. Osmosis is diffusion that involves the transport of water. In osmosis, water moves selectively from high concentration to low concentration.The whole point of the membrane is to allow certain molecules and the ones left behind don't get allowed in. To get to understand more diffusion is the movement of molecules from an area of high concentration to an area of low concentration. Osmosis is the diffusion of water and the main difference between osmosis and diffusion is that osmosis involves water and diffusion can be in the air.
Hypothesis
The glucose will diffuse out of the
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We got a 30 cm piece of dialysis tubing and soaked it in distilled water for a few minutes, then closed the end of the tube with a rubber band creating a bag so in the end of the tube no solution will leak.We then added 15 ml of glucose in the bag then 15 ml of starch solution. We then held the bagan mixed it and recorded the colors. Then we performed benedict's test and had 4 different test tubes that was the control tube, bag tube, and beaker tube and added 1 drop of benedict's reagent. After that we heated the test tubes in a water bath for about 3 …show more content…
The Benedict’s test is to find if there is any glucose and then in our test it indeed indicated that their was glucose by turning red.This supports our hypothesis because it allowed glucose in from the starch and glucose solution. Since there was no change in the second Benedict’s test it shows that it did not let any iodine into the solution.
2) The first test it allowed glucose in due to the fact when we observed its color it was redt. For the dialysis bag the original contents of the bag is starch and glucose, and placed in an I2KI solution. The glucose and I2KI is able to diffuse outside the dialysis bag while the starch is stuck in the bag. This mixture of I2KI and glucose results in the purple color. For the beaker the water and iodine, and placed into it is the dialysis bag of starch and glucose. The mixture of iodine and glucose, keeps the color of the solution orange, since only diffusion is occurring. 3) We can now assume that glucose and iodine molecules are approximately the same size molecules while starch is larger because iodine and glucose could pass through the dialysis bag, while starch molecules could not due to the fact that the the molecules are
These labels indicated the lactose solution that was be placed into the mini-microfuge tubes. The varying lactose ph solutions were obtained. The four miniature pipets were then used, (one per solution,) to add 1mL of the solution to the corresponding mini-microfuge tubes. When this step is completed there were two mini-microfuge tubes that matched the paper towel. Then, once all of the solutions contained their respective lactose solutions, 0.5mL of the lactase enzyme suspension was added to the first mini-microfuge tube labeled LPH4 on the paper towel, and 4 on the microfuge tube. As soon as the lactase enzyme suspension was added to the mini-microfuge tube, the timer was started in stopwatch mode (increasing.) When the timer reached 7 minutes and 30 seconds, the glucose test strip was dipped into the created solution in the mini-microfuge tube for 2 seconds (keep timer going, as the timer is also needed for the glucose strip. Once the two seconds had elapsed, the test strip was immediately removed, and the excess solution was wiped gently on the side of the mini-microfuge tube. The timer was continued for 30 addition seconds. Once the timer reached 7:32 (the extra two seconds accounting for the glucose dip), the test strip was then compared the glucose test strip color chart that is found on the side of the glucose test strip
Osmosis and Diffusion Investigation Aim: To examine the process of osmosis and diffusion. Part A: Step 1: Q1.[IMAGE] Q2. The jiggling motion is visible because the fat globules are constantly being bombarded by smaller particles. [IMAGE] Q3.
The independent variable for this experiment is the enzyme concentration, and the range chosen is from 1% to 5% with the measurements of 1, 2, 4, and 5%. The dependant variable to be measured is the absorbance of the absorbance of the solution within a colorimeter, Equipments: Iodine solution: used to test for present of starch - Amylase solution - 1% starch solution - 1 pipette - 3 syringes - 8 test tubes – Stop clock - Water bath at 37oc - Distilled water- colorimeter Method: = == ==
This cell membrane plays an important part in Diffusion. Cell membrane and Diffusion Diffusion is the movement of the molecules of gas or liquids from a higher concentrated region to a lower concentration through the partially permeable cell membrane along a concentraion gradient. This explanation is in the diagram shown below: [IMAGE] Turgor When a plant cell is placed in a dilute solution or a less concentrated solution then the water particles pass through the partially permeable membrane and fill the cell up with water. The cell then becomes Turgor or hard. An example of this is a strong well-watered plant.
We then cut our potato tubes with the cork borer and cut them with the scalpel so they were the same length and weighed them. We then put one potato tube in each test tube and then added the same amount sugar solution in to each tube. The concentration of sugar solutions varied in each test tube.
In osmosis, water can travel in three different ways. If the molecules outside the cell are lower than the concentration in the cytosol, the solution is said to be hypotonic to the cytosol, in this process, water diffuses into the cell until equilibrium is established. If the molecules outside the cell are higher than the concentration in the cytosol, the solution is said to be hypertonic to the cytosol, in this process, water diffuses out of the cell until equilibrium exists. If the molecules outside and inside the cell are equal, the solution is said to be isotonic to the cytosol, in this process, water diffuses into and out of the cell at equal rates, causing no net movement of water. In osmosis the cell is selectively permeable, meaning that it only allows certain substances to be transferred into and out of the cell.
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
The purpose of this lab was to see firsthand the diffusion of a substance across a selectively permeable membrane. Diffusion is the movement of molecules from an area of high concentration to an area of lower concentration until both concentrations are equal, or as you could more professionally call it, equilibrium. This concept is one that we have been studying in depth currently in Biology class.
I prepared two large test tubes, each should have an inch of KOH pellets on the bottom of the tube. Next, a cotton ball is placed in each of the two test tubes above the KOH to plug the tube. Now one tube is filled to the top with peas, the peas are then removed and weighed to the nearest.1 grams, this is the experimental tube. The control tube is filled with plastic balls to the same height as the experimental tube. Next, a rubber stopper with attached capillary tubing is inserted in each test tube.
However, in order to measure the rates of reaction, sodium thiosulphate and starch are added. Sodium thiosulphate is added to react with a certain amount of iodine as it is made. Without the thiosulphate, the solution would turn blue/black immediately, due to the iodine and starch. The thiosulphate ions allow the rate of reaction to be determined by delaying the reaction so that it is practical to measure the time it takes for the iodine to react with the thiosulphate. After the all the thiosulphate has reacted with the iodine, the free iodine displays a dark blue/black colour with the starch. If t is the time for the blue/black colour to appear, then 1/t is a measure of the initial rate.
The Benedict's Test is used to test the presence of simple sugars in a sample. If sugars are present, a color change will occur from blue to red. However, although the Benedict's test shows the presence of sugars, it cannot accurately determine the concentration of sugar in a sample solution. In our method, we added specific concentrations of glucose to the Benedict's test to use as a chart to estimate the glucose concentration of an unknown solution X. Although this gives a rough estimate of the concentration, it is very inaccurate. For example, the mystery solution X was a pale orange color, which was between the colors in my first and second test tube.
The Effect of Solute Concentration on the Rate of Osmosis Aim: To test and observe how the concentration gradient between a potato and water & sugar solution will affect the rate of osmosis. Introduction: Osmosis is defined as, diffusion, or net movement, of free water molecules from high to low concentration through a semi-permeable membrane. When a substance, such as sugar (which we will be using in the experiment we are about to analyse), dissolves in water, it attracts free water molecules to itself, and in doing so, stops them from moving freely. The effect of this, is that the concentration of (free) water molecules in that environment goes down. There are less free water molecules, and therefore less water molecules to pass across a semi-permeable membrane, through which sugar molecules and other molecules attached to them are too big to diffuse across with ease.
== = This experiment is based on the concept of Osmosis. Osmosis is the diffusion of water molecules from a region of high water concentration to a low water concentration through a semi permeable membrane (in this case, the cell potato cell membrane). The cell walls of the potato cells are semi permeable meaning that water molecules (which are small) can fit through but other bigger molecules such as glucose cannot pass through. The water molecules can flow both ways through the membrane, letting molecules both in and out.
Diffusion is the movement of an element or object across a membrane. Osmosis has to do with the diffusion of water. Osmosis allows water to move from areas of high concentrations to low concentrations. An example of osmosis is blood cells absorbing water. Osmosis is highly important in the survival of cells.
== = Osmosis is the diffusion of water molecules across a partially permeable membrane, from a region of high concentration to a region of low concentration. The partially permeable membrane contains a series of small holes, allowing only water molecules to pass through, as shown in the diagram below. The glucose molecules (represented by red circles) are too big to fit through the membrane. As there are a greater number of water molecules (represented by blue circles) on the left side, there is a steady net flow into the right side with fewer water molecules, i.e. into the stronger solution.