Compare The Absorption Spectrum Of Phenol Red

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By comparing the absorption spectrum of phenol red in basic and acidic solutions, the red (basic) form had a much narrower and higher λ max (560nm) than the yellow form (420nm). As a result, the red form of phenol red was more sensitive with pH changes and it was used in for an assay in part B. In part B, the standard calibration curve was generated in order to determine the concentration of phenol red in the unknown 2B. From the slope, the average concentration of the unknown 2B was 0.001009 M. More importantly, the % difference of unknown trials was low (0.40%), indicating the high precision and reliability of the result. In part C, the pKa of the phenol red can be determined by plotting pH as a function of log ([A-])/([HA]). The pKa and

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