By comparing the absorption spectrum of phenol red in basic and acidic solutions, the red (basic) form had a much narrower and higher λ max (560nm) than the yellow form (420nm). As a result, the red form of phenol red was more sensitive with pH changes and it was used in for an assay in part B. In part B, the standard calibration curve was generated in order to determine the concentration of phenol red in the unknown 2B. From the slope, the average concentration of the unknown 2B was 0.001009 M. More importantly, the % difference of unknown trials was low (0.40%), indicating the high precision and reliability of the result. In part C, the pKa of the phenol red can be determined by plotting pH as a function of log ([A-])/([HA]). The pKa and
pH of the phenol red was around 7.8 when log ([A-] / [HA]) = 0. In other words, since there are equal amounts of [A] and [HA] in the solution, the pKa is found to be equal to the pH. This result further supported the validity of the Henderson-Hassebalch equation.
The analyzed yellow#5 wavelength was determined to 395nm because the actual wavelength 427nm was restricted in the Micro lab. The R2 value of the graph is 0.9827, and the level of data accuracy it indicated extremely weak data correlation. The first one dilution data points excluded from the standard curve because the point is not in the linear curve. The first concentration and absorbance value are the highest point in the graph that cannot connect as linear with another data point. After removing the first data point, the standard curve is clear and make
The color that was chose to be shined through the sample was purple. The spectrophotometer was set at a wavelength of 400nm to represent the purple color. It was zeroed using the blank meaning the spectrophotometer read zero as absorbance amount. The blank consisted of 5mL of water and 2.5 mL AVM and it was placed in cuvette. A solution with a known concentration of 2.0x10-4 M was used in the spectrometer. For this solution, 5 mL of the solution with 2.5 mL of AMV was placed in the cuvette. The cuvette was placed inside of spectrophotometer and the amount of absorbance was recorded. This procedure that involves a solution with a known concentration was repeated for the concentrations:1.0x10-4 M,5.0x10-5 M,2.0x10-5M, and1.0x10-5M.A unknown solution absorbance was measured by putting 5 mL of unknown solution with 2.5 mL AMV in a cuvette. The cuvette was placed in the spectrophotometer and the amount of absorbance was recorded. The procedure that deals with the unknown solution was repeated 2 more times with the same solution and the same amount of solution and AMV. The average of the three unknown solution was calculated and the concentration of the unknown solution was
Hurst, the author of The Scarlet Ibis, uses the color red to symbolize a recurring theme throughout the story which is: Pride can aid, but if not controlled will cause harm. The color red is used throughout the story symbolically, to convey the author’s message. The color in itself has two sides to it; one being that it is a royal color, meaning pride, love, and power. The other is that red represents danger, blood, death, and destruction. Just like the color, pride also has two sides to it. Pride encouraged Brother, the narrator and protagonist of the story, to teach Doodle, his younger brother, to walk. However, pride then began to pull Brother into dangerous territory, and caused harm to Doodle. Pride, like the color red is filled with the yearning to see our loved ones do well. Just like the alternate side of the color red that brings destruction, pride’s alternate side
The task of interpreting William T. Vollmann’s works seems as monumental for the reader as writing the story oneself. The text of “The Visible Spectrum”, in fact, does not feature any extensively challenging vocabulary or particularly thwarting subject matter; yet it would seem that in all of its “objectivity” and “transparency”, there lies no obvious, dominant or intended interpretation. The narrative is ambiguous in its “message” to an infinite degree, and thus the reader must construct its “meaning” given only scraps of discontinuous plot, description and dialogue.
The reaction for pH 5 went off scale at one hundred seconds; its reaction went faster than the other tested pH levels. At twenty seconds, the pH 9 reaction reached 0.244 absorbance, slowly increased, and then slightly dropped to 0.296 by the end of the reaction (Figure 3). The rate of absorbance for peroxidase with pH 9 was 0.0097, so peroxidase activity is least optimal at pH 9. The absorbance rate for pH 3 was 0.0866, and the rate for pH 7 was 0.1426. Because the rate of absorbance for pH 5 was 0.3493, it is the optimum pH level for peroxidase. Highly acidic and alkaline pH levels reduce activity. (Figure
Blue wavelength was used to measure absorbance; using initial data and Beer’s law, calculated molar extinction coefficient was 10953 L∙mol^(-1)∙cm^(-1). Variations of concentration over time were graphed to figure out dissociation of ferroin being first-order reaction and sulfuric acid being zero-order. Thus, under the rate law, the solution’s rate was found to be k〖[Ferroin]〗^1 〖[Sulfuric acid]〗^0, where rate constant k = 9.12x〖10〗^(-4) s^(-1). Half-life was 759.87 sec and Ea (activation energy) was 56.82 KJ.
There are a few changes that should be made to the procedure so that the experiment could generate better results. More time should be allowed to dissolve the tablets as if they are not dissolved colour changes are harder to identify during the titration and also the results are less accurate. The acid used should also be more concentrated as a 0.5mol or 0.3mol would mean a smaller amount of acid would be required.
Just as butterflies transform and evolve, so did Alice Walker’s main character in The Color Purple. Cellie, the main character, had transformed and evolved in the story. Throughout Alice Walker’s novel, The Color Purple, Cellie went through four stages, and transformed from a weak girl into an independent woman. This novel showed how Cellie’s wings were cut in childhood, but she managed to fly with independence.
The romantic red effect occurs when men are more attracted to women displaying the color red. This may be through the flushing of the cheeks during women’s ovulations or wearing red lipstick. The romantic red effect fits within the color-in-context theory because in the context of attraction, heterosexual males are more attracted women in red, while in the context of danger, red may give off a signal of avoidance.
The Effect of Wavelength on Photosynthesis Rate Aim: To be able to To investigate how different wavelengths (colors) of light affect the photosynthetic rate of the synthetic. I will use a pant that is a pond weed called elodea. I will measure the rate of photosynthesis by measuring the amount of o2 given off in bubbles per minute from the elodea. I will do this by placing the Elodea in a test tube with sodium hydrogen. carbonate then I will vary the light wavelength (color) using colored.
After extracting, filtering, and condensing the blue and pink dyes off of the candies, it can be determined that the Blue candy is FD & C Dye Blue 1 and the Pink candy is a mix of Yellow 5 and Red 40. During the solvent evaporation for the blue dye, it was observed that when concentrated, the transparency of the dye was similar to that of Blue 1. During the paper chromatography of Project 2 Session 1, the blue dye showed on the chromatogram to have the highest spot height. Similar to the paper chromatography today which the blue dye was observed having the higher spot height as well. Figure 3 of the Project 2 In-Lab Report displays Blue 1 of having an Rf value of .729. The blue dye off of the candy, as seen in Figure 2, has an Rf value of .657.
This experiment demonstrated the ability of agarose gel electrophoresis to separate the mixture of dyes into their individual components by the application of a combination of dyes to the same sample well. The experiment effectively demonstrated that the dyes where different in structure, energy, and composition. Most of the dyes where negatively charged at neutral pHs and only one with positive charge. The positive charge one moved an opposite direction compared to the other dyes.
In one test tube 0.1M of HCl was added. In the other test tube, 0.1M of NaOH was added. Color change occurred in the NaOH solution. Three agar cubes were cut in 1X1X1 cm, 1X1X2 cm, and 1X0.5X2 cm lengths. Surface area and volume were calculated to get ratios of 6:1, 5:1, and 7:1. Cubes were placed in separate test tubes. In each test tube we covered the cubes with HCl. Time was recorded to show absorption speed when the cube changed color from light pink to clear (table 1).
It changes from blue to red with acids but loses its colour in the presence of certain chemicals, one of which is vitamin C. DCPIP solution can be used to test for the presence of vitamin C in foods. Hypothesis Orange juice has the highest content of vitamin C. Citrus fruits have a higher content of vitamin C. The orange and lemon juice contain more vitamin C than the pineapple juice. Furthermore, as lemons are more acidic than oranges, I predict that the orange juice will contain more vitamin C than the lemon juice. Vitamin C affects, the ph the more vitamin C the higher the ph. Variables Independent Variables Different fruit juices (Pineapple, orange and lemon).
Photosynthetic pigments are essential for life because they allow photosynthesis to occur by capturing sunlight which is then used alongside carbon dioxide and water to form organic compounds such as glucose and oxygen. The pigments allow the conversion of light energy to chemical energy which other organisms can benefit from. Oxygen is utilised by other organisms in aerobic respiration. The different pigments present in the chloroplasts allow a wide variety of wavelengths of light to be absorbed for efficient photosynthesis and provide colours to the plant to attract pollinators.