Cellular Respiration Experiment

Materials and Methods
Materials needed for the cellular respiration experiment were two chambers to trap the CO2, a scale,10 grams of germinated chickpeas, germinated black beans, germinated cranberry beans, germinated red kidney beans, as well as germinated mung beans and glass beans. A Pasco CO2 sensor as well as a computer with SPARKvue software are also required. This experiment contained an independent variable: type of beans and dependent variable: amount of carbon dioxide, this is important to note because it identifies which variables are being altered during the experiment as well as which are not. The independent variable being the species of germinated beans due to the amount of carbon dioxide emitted from each

sample. Assumptions based on the amount of CO2 could then be made about each of the beans cellular respiration. Each group chose their individual experiments they wanted to run and for this one in particular the group tested each species of bean separately over the span of a four-minute period, tracking the CO2 emitted into the chamber by being able to change the species of beans, it is the independent or manipulated variable.

Positive and negative controls for this experiment were a positive control of mung beans and negative control of glass beans. The positive control being from experiment one during the lab section where germinated mung beans, dormant mung beans and glass beads were all examined and compared. Germinated mung beans were concluded to have higher cellular respiration due to the results, emitted the most amount of C02. The negative control of glass beads came from the same experiment except that the glass beads did not emit C02 the way that the germinated mung beans did and therefore could be used as a way to tell if future experiments were not working properly. Positive and negative controls are important because they allow for the researcher to test if their experimenting is functioning correctly by comparing it to results that are to be expected. For instance, if determined that glass beads do not emit C02 that would indicate that it is a negative control and thereby cannot be anything else. Germinated mung beans are a positive control because it could be determined from the results that it produced the highest amount of C02 and therefore had the highest cellular respiration, so all future results could be compared to it to make sure the experiment was not

emitting false-positives or false-negatives.
To run the experiment set up and calibrate the CO2 sensor inside of a chamber using SPARKvue. Set parameters for this experiment (this experiment had the following parameters; sampling mode: periodic, sampling rate unit: seconds, sample rate: 10, condition: stop after duration, value: 4, units: minutes). Select okay and calibrate, this is done by holding the green button on the C02 sensor until it is blinking and letting the experiment run for the allotted four minutes until it stops blinking, when completed the reading should be 400 ppm. For the second part of the experiment use the scale to get 10 g of each type of germinated bean. one at a time place the 10g of black beans into the chamber and place the sensor back into it and run the program for 4 minutes, repeat with the following beans recording data as you go. Save all data and examine graphs to examine the data more in-depth as well as compare results.
Results/Interpretation
Results indicated by this experiment were as follows, the germinated mung beans maintained the highest concentration of C02 while in the chamber. The mung beans were used as the control group so comparing the specimens to the mung beans provided the following information; germinated black beans contained the second highest amount of CO2 when compared to germinated mung beans. After black beans the germinated chick peas provided the most C02 then germinated cranberry beans and finally the germinated red-kidney beans. For the extended species tested, black beans had a slope of 2.07, cranberry beans had a slope of 0.734, chickpeas a slope of 2.06 and finally red-kidney beans a slope of 1.79 which were all compared