Beetroot Experiment
We are trying to find how the temperature affects the rate at which the
anthocyanin pigment leaves the cells, and at what point does the call
wall melt.
Beetroot Experiment
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Task
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We are trying to find how the temperature affects the rate at which
the anthocyanin pigment leaves the cells, and at what point does the
call wall melt.
Please note the two results highlighted found in "test 4" have not
been included into my results, as they are obviously anomalous.
I did the test more that once to the margin of error smaller. For the
first few times I performed the test I could have made a mistake, so
to give my results a better chance of being accurate I did the
experiment four times and took the average of the tests.
A graph of to clearly show the variation my results can be found
overleaf.
As the temperature increases the transmission reading on the colorimeter decreases. From information that I had already gathered I knew it would be around the 60°C mark to 65°C, hence which I decided to add a test at 63°C. This would help me
make a more accurate estimate at what exact temperature the cell wall
melts. My graph shows a decrease of transparency in the water between
the points of 60°C and 70°C. From this I can make the prediction that
between these two points the wall melts.
Analysis
The transparency of the water suddenly decreases between the points of
60°C and 70°C because this is when the cell wall melts. The cause of
the sudden loss of transparency is caused when the wall melts and it
allows a sudden release of the Anthocyanin pigment. So when the wall
melts the pigment is released and the transparency drops - so to find
the temperature at which the cell wall melts we just have to look for
when there is a sudden loss of transparency in the water.
My experiment shows there is a sudden loss of transparency of the
water between 6O°C and 70°C so I know that the cell wall must have
melted between these two points.
Before 60°C and after 70°C the depreciation is not as drastic as between the two points. This is because before 60°C there is no, or a very little amount of the pigment
allowed to exit the cell, which is not enough to alter the transparency in a big way - this is because up to this point the cell wall is still intact, and after 70°C because most of the pigment has already left the cell because the cell wall has melted.
Using the calorimeter, we firstly needed to calibrate the machine; to do this we took a tube of distilled water and tested it; we knew that this should measure 0 because distilled water is completely transparent. We could have done this with any known reference sample. Once we had calibrated the machine we could then test the real samples for their transparency, we tested all five of these samples a total of three times each. Between each different concentration of solution sample we had to re calibrate the machine using the distilled water again, so in total we did 20 colourimetry tests. We gained three results for each concentration of sample and then calculated an average from these three results; these are shown in the table below.
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The results of this experiment are shown in the compiled student data in Table 1 below.
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is 37°C and as soon as you go above this temperature it will cause the
0,74 0,87 1,00 0,49 100... ... middle of paper ... ... some groups had got different leakage of the pigment in the test tubes with water.
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