Permeability of Beetroot Cell Membranes
An experiment to investigate how temperature affects the
Permeability of beetroot cell membranes
Interpretation of Results:
0°C
20°C
30°C
40°C
55°C
0.24
0.28
0.75
0.79
0.22
0.26
0.35
0.41
0.81
From 0°C to 30°C there is a gradual increase of absorbency which shows
that as the temperature increases it is denaturing the cell wall and
cell membrane and allowing the beetroot pigment to leek out into the
distilled water.
As soon as the sample of beetroot is placed into the distilled water
diffusion occurs naturally, which is the net movement of molecules
from a region of high concentration (beetroot sample) to a region of
low concentration (distilled water).
From 40°C upwards there is a sudden increase of absorbency, this is
because the optimum temperature of which enzymes and cells can work at
is 37°C and as soon as you go above this temperature it will cause the
enzymes and cells to denature and cease from working as effectively.
Even if you then lower the temperature again the cell will not regain
its original shape.
Within the phospholipid bi-layer there are proteins, and these
proteins are made up of polypeptide chains which are joined together
by hydrogen, hydrophobic and peptide bonds. Once the temperature has
increased above 40°C the molecules vibrate so energetically that these
bonds break easily and therefore creating holes within the cell wall
and phospholipid bi-layer, and al...
The data we gathered was tested to be as accurate as possible. Our prediction on the solvents did not support our data that we collected. The cause of this could be due to human error when washing the beets or the cutting of the beets. The beets were not perfectly cut the same size, so some beet pieces were bigger than others which can affect the final the final result. We followed each step and followed the time limits cautiously. I can say if we were to redo the experiment our results would be similar because we would attempt to do the experiment as close as we did the first
be too hot or too cold, this is a safety precaution for me as well as
Firstly, when testing temperatures at 30°C and 40°C, the water was. sometimes heated more than needed, so I had to wait until it cooled. down to the required temperature. To avoid this happening, a. thermostatic water bath could have been used, because I could set it. to the required temperature.
In chapter one we are introduced to our narrator, Ponyboy. Ponyboy is raised by his two older brothers Darry and Soda. They’re all apart of a gang called the “greasers” which is joined by Dally, Johnny, Two-bit, and Steve. There is another group called “ socs” which stands for socials, and everyone in that group is very wealthy. One day Ponyboy got jumped by a socs group, but luckily Darry was there to help before anything too serious happened. The first element of literature is characterization. Ponyboy is a keen observer, trying to make sense of the complexities of those around him. At the beginning of the story, he stops and spends several pages giving us brief character description on Steve, Two-Bit, Dally, and Johnny. This is also known as direct characterization. He tells us that Steve is "cocky and smart" ( Hinton 9). Two-Bit can 't stop joking around and goes to school for "kicks" (Hinton 10) rather than to learn. Dallas, he says, is "tougher, colder, meaner" ( Hinton 10) than the rest of them.
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
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How Temperature Effects the Movement of Pigment Through Cell Membranes Abstract The experiment below displays the effects of temperature on the pigment in uncooked beetroot cells. The pigment in beetroot cells lies within the cell vacuole and is called anthocyanin, each vacuole is surrounded by a tonoplast membrane and outside it, the cytoplasm is surrounded by the plasma membrane, therefore the foundation of this experiment lies with the temperature at which the membranes will rupture and therefore leak the pigment. To do this a series of uncooked beetroot cylinders will be exposed to different temperatures and then to distilled water at room temperature (24ºC). The colour of the distilled water is the variable here which will show us, using a colorimeter what temperature the membranes splits using the transmission of the water (light passing directly through and the absorbency (light getting absorbed by the anthocyanin molecules).
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
· The beetroot piece is then placed into a tube of 5 cm of distilled
When I am not using them I will place them away from my experiment and
Water Potential of Potato Cells Aim: To demonstrate the Water Potential of Potato Cells. Objectives: · To show the water potential of potato cells using various measured concentrations of a sucrose solution and pieces of potato. · To record and analyse data to verify observed results. · The method and procedure was carried out as per instruction sheet. Observations: The experiment shows that the lower the concentration of the sugar solution, in the Petri dish, the mass of the potato increased.
The effect of temperature on the beetroot membranes Aim of the research: The aim of this investigation is to determine what kind of effect will the increasing temperature have on the plasma membrane of a beetroot cell. Introduction The beetroot contains a red pigment that is kept in the cells by the membranes. If the membranes are damaged, the pigment “betalain” will leek out. The amount of pigment that leeks out can be assessed, as “betalain” will colour any water that surrounds the cell.
In a 100ml beaker 30mls of water was placed the temperature of the water was recorded. 1 teaspoon of Ammonium Nitrate was added to the water and stirred until dissolved. The temperature was then recorded again. This was to see the difference between the initial temperature and the final temperature.
...ournal articles support the thesis that Obesity is more than an individual’s problems and it affects more than their personal surroundings. It however affects nations economically, began the phenomena of “Globesity” and has been influenced by the body conscious era of time in which we live in. These are ties which we have woven
"The Fight Against Human Trafficking by Speaks115." StudyMode. N.p., n.d. Web. 7 May 2014. .