Aceclofenac Essay

3) LITERATURE REVIEW
Aceclofenac
• Aceclofenac is official in IP & BP but not official in USP.
• Official methods:
Sr.
No. Title
Description Ref.
No.
1. Estimation of
Aceclofenac and re-leated substances.
(IP 2010) • Liquid chromatographic sys-tem:
• Column: a stainless steel col-umn 25 cm x 4.6 mm packed with spherical end-capped octadecylsilane bonded to porous silica (5µm), with a pore size of 10 µm and carbon loading of 19 per cent
• Mobile phase:
A. a 0.112 per cent w/v solu-tion of orthophosphoric acid adjusted to pH 7.0 using a 4.2 per cent solution of sodium hydroxide,
B. 1 volume of water and 9 volumes of acetonitrile
• Flow rate: 1 ml per minute
• UV detection: 275 nm
• Injection volume:10 µL 23
2. Assay of aceclofenac
(IP 2010) • Weigh accurately about 0.3 g and dissolve in 40 ml of me-thanol. Titrate with 0.1 M so-dium hydroxide.
• Determine the end point potentiometrically. Carry out a blank titration.
• 1ml of 0.1 M sodium hydroxide is equivalent to 0.03542 g of C16H13Cl2NO4 23
3. Estimation of aceclofenac and re-leated substances.
(BP 2007) • Liquid chromatographic sys-tem:
• Column:
- size: 1= 0.25 m, 0 = 4.6 mm;
- stationary phase: spherical end capped octadecylsilyl silica gel for chromatography R (5 µm) with a pore size of 10 nm and a carbon loading of 19 per cent;
- temperature: 40 ºC.
• Mobile phase:
- mobile phase A: 1.12 gil solu-tion of phosphoric acid R adjusted to pH 7.0 using a 42 gil solution of sodium hydroxide R;
- mobile phase B: water R, aceto nitrile R (1:9 VIV);
• Flow rate: 1....

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... valida-tion of HPLC method for the determination of pregabalin in capsule. • Column: Hypersil BDS, C8, 150×4.6mm,5µm
• Mobile phase: phosphate buf-fer pH 6.9 and acetonitrile in the ratio of 95:05
• Flow rate: 1 ml/min
• LOQ: 0.6mg/l 41
4. Analytical RP-HPLC method for development and validation of prega-balin and methylcobala-mine in combined cap-sule formulation. • Column: (250 × 4.6mm, 5mcg/ml)
• Flow rate: 1ml/min
• Uv detection: 210nm
• Mobile phase: Ammonium dihydrogen-o-phosphate (buf-fer 6.0), acetonitrile and me-thanol in the ratio of 75:15:10.
• Linearity range:
3200-4800 mcg/ml (pregaba-lin)
16-24 mcg/ml (methylcoba-lamine)
• LOD value:
47.93 mcg/ml (pregabalin)
1.33 mcg/ml (methylcobala-mine)
• LOQ value:
145 mcg/ml (pregabalin)
4.05 mcg/ml (methylcobola-mine) 42