The Importance Of Mycorrhiza

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Mycorrhiza is the symbiotic association between plant roots and soil fungi. Almost all plants form mycorrhiza. It is estimated that c. 74% of all plant species form arbuscular mycorrhiza (AM), 9% forms orchid mycorrhiza, c.2% form ectomycorrhizal (EM) associations and 1% forms ericoid mycorrhiza (Heijden et al. 2015). These fungal-root associations have a key role in terrestrial ecosystems as they regulate nutrient and carbon cycles. Mycorrhizal fungi provide up to 80% of the plant’s N and P to get bread (carbohydrates) and butter (lipids) in return (Rich et al. 2017). The roots both mycorrhizal and non-mycorrhizal are the key source for providing various organic compounds in the habitat in the proximity of, on, and inside the root, which affects …show more content…

However, it certainly indicates that the microorganisms studied are genomes or virtual taxa, using metagenomics method. Studies of rhizosphere microbiome present a holistic view of diversity and interaction across the habitat. Consistent with the terminology used for microorganisms colonizing the human body the collective communities of plant-associated microorganisms are referred to as the plant microbiome or as the plants’ other genome (Qin et al., 2010). In this context, plants are viewed as ‘superorganisms’ which is partly dependent on their microbiome for specific functions and traits. This includes all plant associated microbe habitats such as rhizosphere, spermosphere (seed surface), phyllosphere (leaf surface), and the stem microbiome. Recent application of microbial metagenomics, metatranscriptomics, and metabolomics to plants and their surroundings confirm a key role of mycorrhizal fungi, rhizosphere bacteria and fungi in determining the make-up of rhizosphere microbial community and suggest a world of hitherto undiscovered interactions in the rhizosphere (Dickie et al. 2015). This knowledge is leading to a paradigm-shifting view that plants should be considered as a meta-organism or holobionts instead of isolated …show more content…

Since the taxonomic identification of interacting microflora is not mandatory for a holistic view, the rhizosphere microflora in soils could be characterized in desired details. This also overcomes the difficulties associated with the study of AM fungi which cannot be cultured axenically and their taxonomy is difficult (Powell and Bennett 2015). Recent characterization of barcode sequences (Krugger et al. 2012) and development of dedicated environmental sequence databases for AM fungi (MaarzAMdatabase) (Opik et al. 2010) has made it possible to study and characterize AM fungal genomes in environmental samples (Opik et al. 2016). Powerful amplicon-based deep sequencing (a targeted metagenomics approach) techniques provide more insights into the diversity, structure, and assembly of microbial communities than previous clone library sequencing or PCR-DGGE (denaturing gradient gel electrophoresis) approaches (Guttman et al. 2014) owing to its unprecedented sequencing depth and resolution. 16S ribosomal RNA (rRNA) gene and nuclear ribosomal DNA internal transcribed spacer (ITS) or large-subunit ribosomal RNA (28S LSU) has often been used as barcodes for amplicon sequencing of bacterial and fungal communities (Qin et al 2015)

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