The Enzyme Concentration and The Volume of Juice

The Enzyme Concentration and The Volume of Juice

As the enzyme concentration increases so too does the volume of juice.

This is because as the concentration of enzyme molecules increase

there is more chance of a collision with an apple substrate molecule.

If the chance of a collision is increased then the number of

collisions will increase resulting in a higher rate of reaction.

The enzyme combines with the substrate to form an enzyme-substrate

complex.

Enzyme + Substrate ¬¬¬> Enzyme-Substrate Complex

The enzyme-substrate complex then breaks down to give the product and

releases the enzyme in an unchanged form:

Enzyme-Substrate Complex ¬¬¬> Product + Enzyme

So I can conclude that the rate of reaction is directly proportional

to the concentration of the enzyme. This is clear from the results

that I myself obtained and from the class average. We can see that as

the concentration of the enzyme increases so does the volume of juice

(product) produced from the apple. This is because the more enzymes

that are present the greater the number of active sites available for

the substrate to fit into under the lock-and-key hypothesis, thus

forming a greater number of enzyme-substrate complexes and thus

forming a higher product rate (apple juice).

Enzyme concentration is directly proportional to the rate of reaction

provided the substrate concentration is maintained at a high level and

the pH and temperature are kept constant. We know that the substrate

concentration is maintained at the same level in all samples, this

done by ensuring that all samples are of equal mass and we know that

all the samples were placed in an incubator at 40°C thus ensuring that

the temperature effects the rate of reaction in all samples in the

same way.

Graph: - From 0% ¬> 0.25% concentration we can see the greatest rate

of reaction as there is an abundance of substrate molecules available

to combine with the active site of the enzymes producing a large

gradient on the graph.

As concentration increases from 0.25% ¬> 0.