The Effect of Temperature on the Hatching Success of Brine Shrimps

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The Effect of Temperature on the Hatching Success of Brine Shrimps In this experiment I will study brine shrimps and test the hatching success of them in different temperatures. These crustaceans are small salt water ones; so small that the adults are only about 8 mm in length. They are relatively easy to keep in the laboratory and will produce dormant egg cysts that hatch to produce young shrimp larvae. Hypothesis It is very hard to come to a prediction about this type of experiment as we do not know enough about them to see what affects the reproductive ability of them. Safety 1. Take care with the glassware make sure that they do not break. 2. The magnifying glasses as they are fragile also. Apparatus • 100 cm3 beakers (one for each temperature to be tested) • 100 cm3 de-chlorinated water for each beaker • 2 g sea salt for each beaker • Stirring rod • Access to refrigerator • Water baths or incubators (one for each temperature to be investigated) • Brine shrimp egg cysts • Sheet of graph paper 3 cm _ 4 cm • Magnifying glass • Pair of forceps • Bright light • Fine glass pipette • 40 cm3 beaker of salt water Procedure 1. Decide on a range of temperatures from 5 °C to 35 °C to be tested. 2. Place 2 g of sea salt into a 100 cm3 beaker. 3. Add 100 cm3 of de-chlorinated water and stir until the salt completely dissolves. 4. Label the beaker with the temperature at which it will be incubated. 5. Place a tiny pinch of egg cysts onto a large sheet of white paper. 6. Wet the square of graph paper using a few drops of salt water. Dab the paper onto the white sheet to pick up about 100 eggs. This will look like a tiny shake of pepper. Use a magnifying glass to count the

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