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Industrial process of enzymes
Industrial process of enzymes
Industrial process of enzymes
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Temperature's Effect on the Production of Oxygen From Yeast and Hydrogen Peroxide Planning
Aim
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My aim is to discover how temperature effects the production of oxygen
from yeast and hydrogen peroxide. I will mix yeast with hydrogen
peroxide and use an upturned measuring cylinder to measure how much
oxygen is produced. To find out how much hydrogen peroxide and yeast I
will use, I will carry out pre-tests. This will also aid accuracy of
the final experiment by uncovering potential flaws in the method.
Hypothesis and Theory
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There are many ideas to suggest that the change in temperature will
cause an increase of respiration in yeast. Yeast is a single cell
fungus made up mostly of protein, which has been use for its
applications in fermentation. Yeast, after activation creates the
ferments carbon dioxide and ethyl alcohol by secreting the enzyme
zymase (a complex of 12 enzymes) in the yeast, which acts on simple
sugars such as glucose. The alcohol produced has been used in making
wines and bears and the carbon dioxide produced has been used in
baking as it gets trapped in the dough and causes it to rise.
Enzymes are catalysts which speed up reactions, they are made from
protein and are specific as to which substrate they work on. Enzymes
basically work due to the lock and key theory, where the substrate
substance (the key) fits into the active site on the enzyme and they
bind together, the reaction takes place and the substrate unlocks to
3. The time taken for the yeast to heat up to the temperature of the
For example, substrate concentration, enzyme concentration, and temperature could all be factors that affected the chemical reactions in our experiment. The concentration of substrate, in this case, would not have an affect on how the bovine liver catalase and the yeast would react. The reason why is because in both instances, the substrate (hydrogen peroxide) concentration was 1.5%. Therefore, the hydrogen peroxide would saturate the enzyme and produce the maximum rate of the chemical reaction. The other factor that could affect the rate of reaction is enzyme concentration. Evidently, higher concentrations of catalase in the bovine liver produced faster reactions, and the opposite occurs for lower concentrations of catalase. More enzymes in the catalase solution would collide with the hydrogen peroxide substrate. However, the yeast would react slower than the 400 U/mL solution, but faster than the 40 U/mL. Based on this evidence, I would conclude that the yeast has a higher enzyme concentration than 40 U/mL, but lower than 400
As temperature increases, rate of respiration increases, because particles move faster and with more energy, which in turn means more particles collide with enough energy to react. However, as temperature increases, enzyme stability decreases, so at temperatures above the optimum temperature, the rate will decrease, until all the enzymes have been fully denatured and all the active sites have been lost. Enzymes speed up reactions in organisms. Each enzyme works on a specific substance, called its substrate. The diagram below shows an “E” (an enzyme) catalysing the breakdown of “S” (the substrate) into two different products (“P”).
· I predict that the enzyme will work at its best at 37c because that
Background information:. Enzyme Enzymes are protein molecules that act as the biological catalysts. A Catalyst is a molecule which can speed up chemical reactions but remains unchanged at the end of the reaction. Enzymes catalyze most of the metabolic reactions that take place within a living organism. They speed up the metabolic reactions by lowering the amount of energy.
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide
The Effects of Concentration of Sugar on the Respiration Rate of Yeast Investigating the effect of concentration of sugar on the respiration rate of yeast We did an investigation to find how different concentrations of sugar effect the respiration rate of yeast and which type of concentration works best. Respiration is not breathing in and out; it is the breakdown of glucose to make energy using oxygen. Every living cell in every living organism uses respiration to make energy all the time. Plants respire (as well as photosynthesise) to release energy for growth, active uptake, etc…. They can also respire anaerobically (without oxygen) to produce ethanol and carbon dioxide as by-products.
The purpose of this investigation is to test the effects of multiple sugar substances on the respiration of yeast. Most people think of yeast when they think of what makes bread rise, cheese, alcoholic beverages, or other food products. Another type of yeast can also cause yeast infections, an infection of the skin. Yeasts (Saccharomyces) are tiny, microscopic organisms with a thin membrane and are usually oval or circular-shaped. They are a type of single-celled fungi of the class Ascomycetes, capable of processing sugar into alcohol and carbon dioxide (CO2 ) ; this process is known as fermentation. Fermentation and the products are the main focus points for this experiment being that cellular respiration of yeasts happens via the process of fermentation, which creates by-products of alcohol and CO2. The level of CO2 produced by the yeasts will show how effective each sugar substance is in providing cellular energy for the yeasts.
== == == = This is what I'm going to be changing in the experiment and this will be the temperature and the concentration of the yeast. There are several variables in this experiment, they are: · Amount Used - Too much or too little of the hydrogen peroxide causes the reaction to speed up/slow down producing different amounts of oxygen.
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
The 'lock and key' hypothesis explains how enzymes only work with a specific substrate. The hypothesis presents the enzyme as the 'lock, and the specific substrate as 'key'. The active site binds the substrate, forms a product, which is then released. Diagram 1- a diagram showing the 'lock and key' mechanism works
Enzymes work by lowering the activation energy required by molecules to start the reaction off. Enzymes also react (reversibly) with substrates (The molecule(s) that the enzyme is catalysing) this is done by forming Enzyme-substrate complex, which is then broken down into products. As well as being affected by temperature and pH enzymes optimum rate of reaction is also changed by competitive and non competitive inhibitors. Competitive inhibitors inhibit the enzyme so that enzyme-substrate complex’s cant form until it’s unblocked or there is a change in concentration in substrate, this means it takes longer to reach the optimum rate of reaction.
Temperature increases the rate of reaction between molecules; this will have a profound impact on the rate of respiration. Rate is determined on how fast something is being consumed in a reaction, or how fast something is being crated. The rate of respiration is measured the easiest by the amount of C02 produced by the reaction or by the amount of C02 consumed. The best way to measure gasses is by creating a closed system for an experiment to take place. Consuming oxygen decreases the volume of gas while the production of CO2 increases the production of gas.
At this level there is little activity, as there is little heat and therefore energy for successful collisions. As the heat increases so does the number of collisions and the volume of CO2 produced also increases. From the graph we can see that yeast production does not occur in a linear fashion, but behaves exponentially; as the temperature rises the rate of reaction
The pH of the solution would alter the rate of the reaction if it was