This pH homeostasis lab is used to show how acids and bases react when submerged into different solutions - water, a homogenate, and a buffer. A homogenate is blended up water and the cell tissue. They are used to show how much pH the cells have when adding an acid or base to it. A buffer is a solution that doesn’t change it’s pH level, even when acid or base is added to it. They are important to help all living things maintain homeostasis.
Homeostasis is the internal chemical balance that occurs in all living things. The concentration of hydrogen ions and hydroxide ion creates acids and bases. They are measured using the pH scale, which runs from 0 to 14. In the scale 7 is neutral. To be considered an acid, the concentration of the ion must be between 0 and 6, To be considered a base, the concentration of the ion must be between 8 and 14. Most living things have a concentration range from 5.6 to 8.5. There are many exceptions to this rule, such as the pH
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levels of stomach acid and the enzymes in a small intestine. The human life depends on maintaining homeostasis levels in the body. When trying to find out if a solution is acidic or basic, litmus paper is used.
However, when trying to find the pH of an acid or base, pH paper is used. pH paper is much more accurate than litmus because a specific number can be found to find if the solution is acidic or basic.
In the pH homeostasis lab, 6 experiments were conducted. The hypotheses were: If base is added to water then the pH will increase; If acid is added to water then the pH will decrease; If base is added to homogenate, then the pH will increase; If acid is added to homogenate, then the pH will decrease; If acid or base is added to buffer, then the pH will remain the same. After the experiments were conducted, the graphs were somewhat similar to the hypotheses.
For acid into water, the pH level dropped drastically, from 6 to 3. For base into water, the pH increased from 6 to 12. During the buffer experiments, the pH level stayed the same during the whole experiment for acid and base. It stayed at a pH of 12, hence the name “Buffer
12”. During the homogenate experiment, the pH changed slightly, but not as drastically as the acid and base into water. However, the hypotheses were proven incorrect in that the pH when adding base went up, and the pH when adding acid went down. The homogenate and buffer experiment were similar due to their amount of change, which was little to none. This happened because in the homogenate, there was buffer already in it due to the fact that the homogenate is living cell tissue. From this experiment, knowledge was gained about how the human body reacts with acids and bases, and how bodies can keep homeostasis using buffers. The material that the class has been studying about the chemical reactions in humans correlates with the reactions of acids and bases in humans. Homeostasis is an important part of the survival of humans, and knowing how it works with the human body will help keep the human race stable and healthy.
This is an experimental lab that tested if drinking water passes the United States maximum phosphate standard. The results of this lab can help the American who drink the water know if there are too much phosphate in the water. Each group made a Potassium phosphate dilution from a stock solution. The concentration of the solution that needed to made affected the amount of Potassium phosphate that was diluted. To create a calibration curve, each group used the different concentrated Potassium phosphate solutions in their test. The lab utilized a spectrophotometer to figure out the absorbance of the five different Potassium phosphate solution and the absorbance of an unknown concentration solution. The absorbance of the unknown solution was used
15ml of Buffer Solution at pH 8.4 produced the amount of oxygen required in 0.44cm³ per second. On the other hand, 15ml of Buffer Solution at pH 4.4 produced this amount of oxygen in 1.45cm³ per second. We can clearly see that when the Buffer Solution's pH concentration is increased, this has the same effect on the speed of the reaction, which is the effect of pH on the
Most substances fall on a scale ranging from the most acidic to the the most basic with neutral substances falling somewhere in the middle. Scientists call this the pH scale. pH levels are measured in numbers,0 to 14. The closer a substance is to zero the more acidic it would be. The closer to 14 the more basic a substance would be.Now what defines an acid and a base, one might ask? There are three ways of defining acids, each singling out a specific property. The first theory is the Arrhenius Theory with states, that an acid is a substance that produces the ion H+ when in a water solution, while a base is a substance which produces the ion OH- when in a water solution. Examples of an Arrhenius acid are HCl and HNO3. Examples of an Arrhenius base are NaOH and AlOH3.
I decided to experiment with pHs within the range pH 2 to pH7, as I
Alkaline Phosphatase (APase) is an important enzyme in pre-diagnostic treatments making it an intensely studied enzyme. In order to fully understand the biochemical properties of enzymes, a kinetic explanation is essential. The kinetic assessment allows for a mechanism on how the enzyme functions. The experiment performed outlines the kinetic assessment for the purification of APase, which was purified in latter experiments through the lysis of E.coli’s bacterial cell wall. This kinetic experiment exploits the catalytic process of APase; APase catalyzes a hydrolysis reaction to produce an inorganic phosphate and alcohol via an intermediate complex.1 Using the Michaelis-Menton model for kinetic characteristics, the kinetic values of APase were found by evaluating the enzymatic rate using a paranitrophenyl phosphate (PNPP) substrate. This model uses an equation to describe enzymatic rates, by relating the
The next week was dedicated to the titration of household supplies. For this, we used two sodas, Cheerwine, and Diet Coke, as well as dish soap. Dish soap, along with many other household cleaning agents, has buffering properties. A buffer acts as a pH stabilizer. It is a combination of a weak acid and its conjugate base, or a weak base combined with its conjugate acid. If a strong acid ion is added, the buffer simply replaces it with a weak acid ion, therefore causing little change in the pH of the solution. Household cleaning products usually have some form of a buffer, because otherwise, they would burn skin to touch. As expected, the two sodas were originally acidic, while the soap was basic.
Acid-Base Titration I. Abstract The purpose of the laboratory experiment was to determine equivalence. points, pKa, and pKb points for a strong acid, HCl, titrated with a. strong base, NaOH using a drop by drop approach in order to determine. completely accurate data. The data for this laboratory experiment is as follows.
The experiment is aimed at giving a better understatement of osmosis process and the different conditions in which osmosis occurs.
Methodology: The experimenter used two ten gallon tanks. One tank will be used for the controlled group and the other tank will be used for the experimental group. Each tank will have two pounds of sand spread among the bottom of the tank along with rocks and artificial habitats to add nitrogen to the tanks. To add optimal living conditions for the oceanic life water filtration systems, temperature regulator, circulation systems, and a light to mimic the sun’s rays were added to each tank. At all times both tanks had a temperature of 75 degrees F. This experiment was done over a three month period. The first month was to allow the nitrogen cycle to occur. This allows the fish to be exposed to the water without having stress reactions due to unhealthy living conditions due to the nitrogen. Once the first month was complete six fish was added to both tanks. Two tangs, two damsels, and two clownfish. At first both tanks had a pH level of 8.2, ideal living conditions. After one week the experimental group was exposed to a pH level of 8.6. After two weeks it was raised to 9. Two weeks later it was raised to 9.3. The final raise was done two weeks after making the pH level 9.5. The final week of the experiment the pH lev...
strong acid or base does not necessarily yield a drastic jump in pH. The acid
Some improvements to the experiment might be using Na Acetate or Na Citrate as buffers instead of KHPO4. The pH ranges are 4.5-5.5 and 4.7-5.5, respectively. This range falls closer to the ideal pH of 5, then KHPO4 (pH
For the solid sodium chloride, using distilled water will make it an aqueous solution. Just like before using red and blue litmus paper will only indicate that the sodium chloride is neutral. The same can be done for sodium carbonate since it is soluble in distilled water, making it easy to determine whether it is an acid or base when using litmus paper. In this case, sodium carbonate is a base so red litmus paper will turn blue when the solution of sodium carbonate is dripped on to it.
Initially, before any NaOH is added, the pH of HCl is low because it contains mainly H3O +. As NaOH is added, H3O+ is slowly used by OH- because of dissociation of NaOH. The analyte remains acidic but the pH starts to increase as more NaOH is added.
Acid-Base balance is the state of equilibrium between proton donors and proton acceptors in the buffering system of the blood that is maintained at approximately pH 7.35 to 7.45 under normal conditions in arterial blood. It is important to regulate chemical balance or homeostasis of body fluids. Acidity or alkalinity has to be regulated. An acid is a substance that lets out hydrogen ions in solution. Strong acid like hydrochloric acid release all or nearly all their hydrogen ions and weak acids like carbonic acid release some hydrogen ions.
Almost every solution one may find will have either acidic or basic qualities. A lot of the things that human being eat and drink have different pH levels, which is why it is so important for humans to be knowledgeable on the difference between acids and bases to be able to balance the pH levels in their bodies to remain healthy. For one to determine whether or not a substance is an acid or a base, they first must know the types of ions the substance contains. Due to how wide the range is from a very acidic solution to a very basic solution scientists created a scale, the pH scale, to measure how acidic or basic a substance is more easily.