Microscopy

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Microscopy

Summary

The entire exercise was all about caring for and using the microscope. We performed various activities that enabled us to familiarize ourselves with the parts and uses of the microscope. Using the microscope, we observed different objects such as cotton, silk, hair, and even a letter "e." We viewed it using both the low power objective and the high power objective. We did these activities in order to better understand how the compound light microscope works and also to learn about the different procedures in setting up a slide. The results of the activity made us learn how to operate the microscope efficiently. I found out that the iris diaphragm controls the light and the uses of the LPO and HPO. I also noticed that the view of the object being illuminated is inverted.

Abbreviations

LPO- Low power objective

HPO- High power objective

Introduction

There are a lot of things not visible to the naked eye. What most of us don't realize is that there is a world full of wonders out there; A world full of diversity and "magic." A world called microscopy. It's amazing how one single device called the microscope can let us view things that are too small for the human eye. A microscope has the power to magnify object from 100x to as much as 1000x or higher. In fact, there are many microscopes, each with their own unique capability. Examples of these are the atomic force microscope, scanning probe microscope, and electron microscope. Microscopy is the technique in which a person is able to view images of structures that are too small for the naked eye. In light microscopy, light is reflected through a series of lenses that eventually illuminate the object being seen.

The objectives of the exerc...

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...th the different parts and uses of the microscope. I conclude that compound light microscopes give inverted images of the object because of the reflected light; and that the passage of this light is controlled by the iris diaphragm. I also conclude that each part of the microscope is significant for it to function properly and effectively.

Literature cited

http://www.life.umd.edu/CBMG/faculty/wolniak/wolniakmicro.html

http://en.wikipedia.org/wiki/Microscopy

http://nsm1.fullerton.edu/~skarl/EM/Microscopy/LightMicroscopy.html

http://science.howstuffworks.com/light-microscope.htm

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