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Lab 7- fermentation
Fermentation by yeast experiment
Fermentation by yeast experiment
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Recommended: Lab 7- fermentation
For this experiment, we combined yeast, water, and other ingredients to see the outcome of fermentation. The purpose of this experiment was to see what would happen when yeast was combined with other materials. The learning goal was to show how yeast reacts with other materials and how it shows alcoholic fermentation. In the experiment, we were able to look under a microscope and see how the yeast was woken up when it was exposed to water.
In the experiment when we combined yeast with other materials such as water, grass, sugar, and cornmeal, it reacted creating CO2. When this reaction happened it caused the Ziploc bags to blow up and fill with many air bubbles. In our group, bag A which contained yeast and water rose the most. Its final
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Some of which could have been prevented, while others not so much. One of the main errors in our group that seemed to occur was that the warm water that was being passed around, became colder as it reached the last of the bags. This could have greatly impacted our experiment simply because the temperature of the water could play a very big role in the fermentation process taking place. Another error that was found to take place was human error. The way that the experiment was conducted was that we all measured our own individual bags. In this, someone could have simply read the measurements wrong, causing an incorrect result. From this, we have learned to try and be as accurate as possible and make sure to be taking the correct measurements. For a further experiment, I would recommend just a few changes to try and prevent any human error. Next time we could assign one person to each table to be accurately measuring the height of the bags to make the process more efficient. In our group, the possibility of human error was pretty high so assigning one person could make the experiment more precise. Overall, the lab seemed to be a fairly good representation of the alcoholic fermentation process that occurs when yeast, sugar, and water are
Table 6 shows the results of the biochemical tests. The isolate can obtain its energy by means of aerobic respiration but not fermentation. In the Oxidation-Fermentation test, a yellow color change was produced only under both aerobic conditions, indicating that the EI can oxidize glucose to produce acidic products. In addition to glucose, the EI can also utilize lactose and sucrose, and this deduction is based on the fact that the color of the test medium broth changed to yellow in all three Phenol Red Broth tests. These results are further supported by the results of the Triple Sugar Iron Agar test. Although the EI does perform fermentation of these three carbohydrates, it appears that this bacterium cannot perform mixed acid fermentation nor 2,3-butanediol fermentation due to the lack of color change in Methyl Red and Vogues-Proskauer
In this experiment, there were several objectives. First, this lab was designed to determine the difference, if any, between the densities of Coke and Diet Coke. It was designed to evaluate the accuracy and precision of several lab equipment measurements. This lab was also designed to be an introduction to the LabQuest Data and the Logger Pro data analysis database. Random, systematic, and gross errors are errors made during experiments that can have significant effects to the results. Random errors do not really have a specific cause, but still causes a few of the measurements to either be a little high or a little low. Systematic errors occur when there are limitations or mistakes on lab equipment or lab procedures. These kinds of errors cause measurements to be either be always high or always low. The last kind of error is gross errors. Gross errors occur when machines or equipment fail completely. However, gross errors usually occur due to a personal mistake. For this experiment, the number of significant figures is very important and depends on the equipment being used. When using the volumetric pipette and burette, the measurements are rounded to the hundredth place while in a graduated cylinder, it is rounded to the tenth place.
Possible sources of error in this experiment include the inaccuracy of measurements, as correct measurements are vital for the experiment.
The author directly appeals to his audience of teachers by writing about how useful the demonstration would be in a classroom. The author’s tone is formal and academic in nature, without heartfelt appeals or attempts to persuade by emotion. In the span of only a few pages, L.B. The church has given us an overview of the winemaking process. He has done so with sufficient detail for those in the chemistry community to follow along, yet still in a cursory enough manner as to not bog them down with the unnecessary. Written as if it were the procedure of an experiment, he has given enough information for the experiment to be repeated, tested, validated and improved upon.
The purpose of this investigation is to test the effects of multiple sugar substances on the respiration of yeast. Most people think of yeast when they think of what makes bread rise, cheese, alcoholic beverages, or other food products. Another type of yeast can also cause yeast infections, an infection of the skin. Yeasts (Saccharomyces) are tiny, microscopic organisms with a thin membrane and are usually oval or circular-shaped. They are a type of single-celled fungi of the class Ascomycetes, capable of processing sugar into alcohol and carbon dioxide (CO2 ) ; this process is known as fermentation. Fermentation and the products are the main focus points for this experiment being that cellular respiration of yeasts happens via the process of fermentation, which creates by-products of alcohol and CO2. The level of CO2 produced by the yeasts will show how effective each sugar substance is in providing cellular energy for the yeasts.
One possible source of experimental error could be not having a solid measurement of magnesium hydroxide nor citric acid. This is because we were told to measure out between 5.6g-5.8g for magnesium hydroxide and 14g-21g for citric acid. If accuracy measures how closely a measured value is to the accepted value and or true value, then accuracy may not have been an aspect that was achieved in this lab. Therefore, not having a solid precise measurement and accurate measurement was another source of experimental error.
There were five test solutions used in this experiment, water being the control, which were mixed with a yeast solution to cause fermentation. A 1ml pipetman was used to measure 1 ml of each of the test solutions and placed them in separated test tubes. The 1 ml pipetman was then used to take 1ml of the yeast solution, and placed 1ml of yeast into the five test tubes all containing 1 ml of the test solutions. A 1ml graduated pipette was placed separately in each of the test tubes and extracted 1ml of the solutions into it. Once the mixture was in the pipette, someone from the group placed a piece of parafilm securely on the open end of the pipette and upon completion removed the top part of the graduated pipette.
In our Biology Lab we did a laboratory experiment on fermentation, alcohol fermentation to be exact. Alcohol fermentation is a type of fermentation that produces the alcohol ethanol and CO2. In the experiment we estimated the rate of alcohol fermentation by measuring the rate of CO2 production. Both glycolysis and fermentation consist of a series of chemical reactions, each of which is catalyzed by a specific enzyme. Two of the tables substituted some of the solution glucose for two different types of solutions. They are as followed, Table #5 substituted glucose for sucrose and Table #6 substituted the glucose for pH4. The equation for alcohol fermentation consists of 6 Carbons 12 Hydrogens 6 Oxygen to produce 2 pyruvates plus 2 ATP then finally the final reaction will be 2 CO2 plus Ethanol. In the class our controlled numbers were at Table #1; their table had 15 mL Glucose, 10 mL RO water, and 10 mL of yeast which then they placed in an incubator at 37 degrees Celsius. We each then measured our own table’s fermentation flasks every 15 mins for an hour to compare to Table #1’s controlled numbers. At
Investigating the Effect of Temperature on the Fermentation of Yeast To fully investigate the effect of temperature on the rate of fermentation of yeast Background Information Yeast is a single-cell fungus, occurring in the soil and on plants, commonly used in the baking and alcohol industries. Every living thing requires energy to survive and through respiration, glucose is converted into energy. There are two types of respiration available to living cells are: 1.
This lab has two sections. The first section deals with fermentation. The purpose of the fermentation lab is to alter 5 different independent variables (temperature, acid ph, alkali ph, enzyme concentration, and substrate concentration), to learn about their effects on the ongoing process of fermentation.
Hypothesis If the type of yeast affects the rate of ethanol fermentation, then Non-expired Bread Machine will result in the fastest rate followed by Non-expired Quick Rise, Non-expired Traditional, and expired Quick Rise because Bread Machine has the smallest yeast granule size allowing it to dissolve faster, the pellet size of Quick Rise is smaller than Traditional suggesting that Traditional has a slower rate, and expired yeast loses its potency over time so it has the slowest rate of ethanol fermentation. Procedure The dependent variable is the rate of ethanol production. As sucrose reacts with the activated yeast, carbon dioxide gas will be produced. The dependent variable was measured by observing the amount of carbon dioxide gas produced.
For this lab, we determined the amount of ATP that was produced from baker’s yeast from the amount of CO2 produced when glucose was reduced. We then determined whether the fermentation process was aerobic or anaerobic.
Lastly, the 10% sucrose solution’s depth of carbon dioxide bubbles ranged from 0-65mm. Another way that we measured the rate of fermentation was through the circumference of the balloon, as an indicator for the carbon dioxide released. The 0% sucrose solution’s range of circumference was 0-61mm. The 1% sucrose solution’s circumference ranged from 0-76mm. The 5% sucrose solution’s circumference ranged from 0-94mm.
Yeasts, can be differentiated with moulds. By fermentation, yeast changes carbohydrates to carbon dioxide and alcohols It’s an important organism in modern cell biology research, and is the most researched microorganisms.
There is also the potential of human error within this experiment for example finding the meniscus is important to get an accurate amount using the graduated pipettes and burettes. There is a possibility that at one point in the experiment a chemical was measured inaccurately affecting the results. To resolve this, the experiment should have been repeated three times.