Lab Report Enzyme Activity

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Enzyme activity experiment for this lab shows that in the decomposition of hydrogen peroxide with the enzyme catalase, the whole banana reaction produced 6.8 mL of oxygen while the diced banana produced 8.0 mL of oxygen gas (see table 1.0). The increase in the amount of oxygen produced when the diced banana reacted was because of the increase in the surface area of the diced banana which increased the rate of catalyzing the reaction with the hydrogen peroxide. The second experiment was on the effect of temperature on the liver. The result of the experiment proved the hypothesis that 37 ºC water bath would have the highest temperature since the optimum PH for catalase is 7 and the temperature is 37 ºC. The boiled enzyme had the lowest …show more content…

The last experiment was conducted to determine the effect of PH on the enzyme activity. The experiment corresponded with the hypothesis that at PH 7 the reaction would be faster. The reaction rate of PH 3 was extremely slow because the PH range is not close to the optimum PH of 7 and PH 10 was very high that it denatured the enzymes. That proves that extreme high and low PH levels would induce a low enzyme activity because the hydrogen bonds have been destroyed. (see table 4.0)

Discussion

The results that was obtained from the various experiments supported our hypothesis. In experiment 1 the whole banana was dropped into the test tube containing the solution of 3% hydrogen peroxide(H2O2). The whole banana reacted with the solution of hydrogen peroxide to form white fumes at the top of the test tube. The level of water was going down slowly while bubbles of water was going up slowly as well. When the diced banana was dropped in the test tube, the level of water was going down vigorously, and bubbles of water was rising vigorously as well. The observation behind this experiment was that the experiment is …show more content…

Based on the experiments conducted, I learned that enzymes are greatly needed as a catalyst to bring about a specific biochemical reaction. Enzymes speed up the rate of chemical reaction by lowering the activation energy. From my observations, I learned that most enzymes require a specific environmental condition for them to function effectively. Diced banana increased in the amount of oxygen it produced because of the increase in the surface area. Also, when the liver was exposed to hydrogen peroxide to determine the effect of temperature, PH and its reusability, the observations deduced was that enzymes work best at a specific pH which is known as the optimal pH and perform at their best in a small range of temperature. Finally, I learned that enzymes are not changed by the reactions the catalyze and hence the are not denatured or altered during a chemical

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