Enzyme Peroxidase Lab Report

609 Words2 Pages

Enzymes are catalysts which lower the activation energy of chemical reactions, thus making them occur more rapidly. Enzymes are in constant use because of their ability to increase the rate of the reaction without permanent alteration. Each enzyme has a different rate of reaction as well as a maximum speed; you can find this number by adding substrate into the concentration until the speed remains constant. The maximum velocity divided by two gives you the Km. In this experiment the efficacy of the enzyme peroxidase was tested. Using differing amounts of substrate in each reaction a spectrophotometer was utilized to observe how fast each reaction produced product. By observing the absorbance for the color change of the oxidized element we …show more content…

Enzymes act as biological catalysts and are characterized by long chains of amino acids bound together by peptide bonds. Enzymes are seen in all living cells and the metabolic processes in which they convert nutrients into energy and new cells. They also assist in the breakdown of food materials. The reactants of enzyme catalyzed reactions are termed substrates. Each enzyme is quite specific in character, acting on specific substrates to produce specific products. The central approach for studying the mechanism of an enzyme-catalyzed reaction is to determine the rate of the reaction and its changes in response to the changes in parameters such as substrate concentration, enzyme concentration, pH, temperature all of this is known as enzyme kinetics. The substrate concentration, is one of the most important parameters that affects the rate of a reaction that catalyzed by an enzyme. To determine the effectiveness of a catalyst we use the Michaelis-Mentenconstant (Km). The Km is believed to provide a measure of the substrate concentration that is required for catalyst to occur(Berg, 2016). Km can indicate the efficiency of an enzyme, if the constant is low

Horseradish Peroxidase Lab Report
1394 Words | 3 Pages
Data from Table 1. confirms the theory that as the concentration of glucose increases so will the absorbance of the solution when examined with the glucose oxidase/horseradish peroxidase assay. Glucose within the context of this assay is determined by the amount of ferricyanide, determined by absornace, which is produced in a one to one ratio.1 Furthermore when examining the glucose standards, a linear calibration curve was able to be produced (shown as Figure 1). Noted the R2 value of the y = 1.808x - 0.0125 trend line is 0.9958, which is statistically considered linear. From this calibration curve the absorbance values of unknowns samples can be compared, and the correlated glucose concentration can then be approximated.
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Enzyme Peroxidase Lab Report
454 Words | 1 Pages
In this experiment the enzyme peroxidase and the substrate hydrogen peroxide were not mixed initially, instead they were both placed in separate tubes and were incubated at a specific temperature, to prevent hydrogen peroxide from undergoing any reaction with peroxidase until they both acquire the required temperature.
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Kinetics Of Ester Hydrolysis Catalyzed By Digestion
1506 Words | 4 Pages
The purpose of the experiment is to study the rate of reaction through varying of concentrations of a catalyst or temperatures with a constant pH, and through the data obtained the rate law, constants, and activation energies can be experimentally determined. The rate law determines how the speed of a reaction occurs thus allowing the study of the overall mechanism formation in reactions. In the general form of the rate law it is A + B  C or r=k[A]x[B]y. The rate of reaction can be affected by the concentration such as A and B in the previous equation, order of reactions, and the rate constant with each species in an overall chemical reaction. As a result, the rate law must be determined experimentally. In general, in a multi-step reac...
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Horseradish Peroxidase Experiment
513 Words | 2 Pages
Enzymes are used to carry out reactions in a rapid manner otherwise the reaction would occur very slowly thus not being able to sustain life. Enzymes bind to a substrate that is specific to their task and then conforms into a product that is needed; the enzyme is then able to catalyze more of the same reaction. Enzymes and substrates act as a lock and a key since enzymes are made for a specific substrate and is able to form an enzyme-substrate complex (Department of Biology). Thus changes of the shape of an enzyme can inhibit its ability to catalyze a reaction. If the enzyme shape is alternated due to environmental conditions, it is denatured and can no longer act as a catalyst. Peroxidase is the type of enzyme used in this
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Catalase Lab Report
2256 Words | 5 Pages
This hurdle is called the activation energy of the reaction. [IMAGE] By decreasing the activation energy, more substrate is changed to product in a certain amount of time. That is, the enzyme increases the rate of the reaction. [IMAGE] The activity of catalase can be measured by finding the rate of which the oxygen gas is released from the breakdown of Hydrogen Peroxide.
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Enzyme Concentration Lab Report
731 Words | 2 Pages
My results did not completely support my hypothesis, while I was correct about pH, temperature, enzyme concentration and inhibitors I was incorrect about substrate concentration. I originally believed that increasing substrate concentration
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Investigating the Effect of Substrate Concentration on Catalase Reaction
4867 Words | 10 Pages
Background information:. Enzyme Enzymes are protein molecules that act as the biological catalysts. A Catalyst is a molecule which can speed up chemical reactions but remains unchanged at the end of the reaction. Enzymes catalyze most of the metabolic reactions that take place within a living organism. They speed up the metabolic reactions by lowering the amount of energy.
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Enzymes Lab Report
725 Words | 2 Pages
In biology class, we were learning about enzymes. Enzymes are proteins that help catalyze chemical reactions in our bodies. In the lab, we were testing the relationship between the enzyme catalase and the rate of a chemical reaction. We predicted that if there was a higher percentage of enzyme concentration, then the rate of chemical reaction would increase or it would take less time. We placed 1 ml of hydrogen peroxide into four depressions. Underneath the first depression, we place 1 ml of 100% catalase and make 50% dilution with 0.5 ml of water. We take 50% of that solution and dilute with 0.5 ml of water and we repeat it two more times. there were four depressions filled with catalase: 100%, 50%, 25% , 12.5 % with the last three diluted
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Enzyme Peroxidase Lab Report
808 Words | 2 Pages
Materials used in the experiment included 5-7 g of the potato tissue, 50ml of 2.0M phosphate buffer coffee filter and guaiacol dye.
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Investigating the Effect of Enzyme Concentration on the Hydrolysis of Starch with Amylase
4181 Words | 9 Pages
Enzymes have the ability to act on a small group of chemically similar substances. Enzymes are very specific, in the sense that each enzyme is limited to interact with only one set of reactants; the reactants are referred to as substrates. Substrates of an enzyme are the chemicals altered by enzyme-catalysed reactions. The extreme specific nature of enzymes are because of the complicated three-dimensional shape, which is due to the particular way the amino acid chain of proteins folds.
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Potato Enzyme Lab Report
597 Words | 2 Pages
An enzyme is a catalyst made of protein and it’s purpose is to expedite the period of a reaction. Enzymes are commonly large and have a surface that is purposed with grabbing other molecules, their surfaces contain hydrophilic amino acids which result in the enzymes being soluble in water. The 3D structure of an enzyme allows a substrate to connect to it’s active site and this allows a product to be formed from its binding. As the substrate and enzyme bind, the substrate changes it’s shape and discontinues the product being formed.
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Lactase Enzyme Lab Report
1772 Words | 4 Pages
Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is important that a specific enzyme is present during the process. For example, lactase must be able to collaborate with lactose in order to break it down (Madar & Windelspecht, 105).
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Peroxidase Lab
1282 Words | 3 Pages
Research on the Optimal Environment for Peroxidase to Survive with a focus on Temperature, pH, and Concentration Introduction Enzymes play a major role in virtually every single ecosystem as a conductor of reactions. They are important because without them reactions that need to take place in these cells would occur to slowly for DNA replication to occur as well as other reactions. The enzyme chosen for this experiments was peroxidase.
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Enzyme Catalyzed Reaction Lab Report
514 Words | 2 Pages
In this lab, it was determined how the rate of an enzyme-catalyzed reaction is affected by physical factors such as enzyme concentration, temperature, and substrate concentration affect. The question of what factors influence enzyme activity can be answered by the results of peroxidase activity and its relation to temperature and whether or not hydroxylamine causes a reaction change with enzyme activity. An enzyme is a protein produced by a living organism that serves as a biological catalyst. A catalyst is a substance that speeds up the rate of a chemical reaction and does so by lowering the activation energy of a reaction. With that energy reactants are brought together so that products can be formed.
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The Industrial Application of Enzymes
1386 Words | 3 Pages
The Industrial Application of Enzymes Enzymes are naturally occurring biological molecules found in all living organisms, plant, animal and microorganisms such as bacteria. All enzymes are proteins and, as with all proteins, are made up of a chain or polymer of amino acids held together by peptide bonds. This chain coils to form a specific three-dimensional globular shape, which, typically, means an enzyme will only work with one specific substrate. The purpose of an enzyme is to lower the activation energy required for biochemical reactions to take place. As a result a reaction catalysed by an enzyme will be much more efficient at breaking down a substrate into its products and consequently are used in industry for just this purpose.
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Enzyme Peroxidase Lab Report

Horseradish Peroxidase Lab Report