Investigating the Concentration of Plant Cell Sap Using Potato Tuber Cells
An experiment to work out the concentration of plant cell sap using
potato tuber cells
First of all and the most important are the safety precautions we will
take throughout this experiment
· Wear safety glasses to protect eyes
· Handling the acids carefully
· Careful while using kitchen knife
(And all the simple things such as not running etc.)
Another important aspect before we start is making the experiment a
fair test. This includes making sure the volume of each acid is the
same using a measuring cylinder. The same temperature for each
individual solution will be the same as they will be done one after
the other and left for the same amount of time in the same place. A
significant temperature change in between each one is unlikely to
occur, especially indoors. Also the 6 potato chips will be the same
size (using a ruler). The only difference between each solution will
be the concentration.
Apparatus -
· Cork borer
· Potato
· Measuring cylinder
· Glass test tubes
· 6 petri dishes with lids
· Kitchen knife
· White tile
· Weighing scales
· Different sugar concentrations/molarities (0.1M, 0.2M etc)
· Washable pen
Useful Science involved -
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Osmosis
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Osmosis is the diffusion of water from a dilute solution to a more
concentrated solution through a semi-permeable membrane. The water
moves from where it's a high concentration to where it's a low
concentration. Concentration of the water is the only thing that
matters; the solute molecules (e.g. sugar) can't pass through the
membrane. The effect of all of this is to gradually dilute the sugar
solution
Example -
Prediction - After taking a close look at my preliminary experiment, I
predict that the molarity of the potato will be 0.3M and the mass will
increase if the solution is less than 0.
For both experiments, data were collected for thirty seconds.
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