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Investigating Osmosis in Potato Cells
Planning:
Aim:-
To investigate the effect of different concentrations of sucrose
solution, on potato cells
Osmosis:-
Osmosis is the passage of high water concentration through a
semi-permeable membrane to a region of low water concentration, i.e.
the passage of water.
Explanation of osmosis
For osmosis to occur there must be;
semi-permeable membranes are very thin layers of material (cell
membranes are semi-permeable) which allow some things to pass through
them but prevent other things from passing through. Cell membranes
will allow small molecules like Oxygen, water, Carbon Dioxide, etc. to
pass through. Cell membranes will not allow larger molecules like
Sucrose, etc. to pass through.
Regions of high concentrated water are either a very dilute solution
of something like sucrose or pure water. In each case there is a lot
of water, there is a high concentration of water.
Regions of low concentrated water are a concentrated solution of
something like sucrose. In this case there is much less water.
If the solution surrounding the cell has a higher water concentration
than the cell, the cell will gain water by osmosis. Water molecules
are free to pass across the cell membrane in both directions, but more
water will come into the cell than will leave. The cell is likely to
swell up and become 'turgid'.
If the solution is exactly the same water concentration as the cell
there will be no movement of water across the cell membrane. Water
crosses the cell membrane in both directions, but the amount going in
is the same as the amount going out. The cell will stay the same size.
If the solution has a lower concentration of water than the cell the
cell will lose water by osmosis. Again, water crosses the cell
membrane in both directions, but this time more water leaves the cell
than enters it. Therefore the cell will shrink and become 'flaccid'
Potato plant cells:-
Plant cells always have a strong cell wall covering them.
This process can also be defined as osmosis, which is the diffusion of water molecules across a membrane. (Unknown, 2) When we first got the egg it would be an isotonic solution meaning that it had equal tension (Unknown,1) which would mean that the same amount was inside the cell as outside of it. The purpose of the experiment was to learn about diffusion, concentration gradient, passive transport, equilibrium, osmosis, tonicity, hypertonic solutions, hypotonic solutions, isotonic solutions, and osmoregulation. If I put the egg in corn syrup, then the egg will deform because it has been placed in a hypertonic solution.
I also predict that solution concentration 0.3 will be isotonic because of the pressure potential. As the water moves into the cell it pushes up against the cell wall this is called the pressure potential and it increases the water potential until an equilibrium is reached, and because the cell is so inelastic it takes very little water to achieve this. That's why I believe in-between 0.3 and 0.4 will be isotonic as it is very close to pure water. Background Information Osmosis - The net movement of water molecules from a region of high water potential to a low water potential, through a selectively
In the diagram below on the right, we see two solutions divided by a partially/selectively permeable membrane (i.e. one that is porous, but allows water molecules through faster than dissolved substances). Text Box: Originally, the two solutions were; pure water, on the left, and sugar solution with a high sugar concentration, on the right. The pure water solution is said to have higher water potential than the concentrated sugar solution, because the water will flow from the area of high concentration of free water molecules (the dilute solution), to the area of low concentration of free water molecules (the concentrated solution). In other words, to the sugar solution.
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
There will be a net movement in these types of solution. The molecules will move from the hypotonic solution into the hypertonic solution. The third way a substance can cross the cell membrane is through facilitated diffusion.
In osmosis, water can travel in three different ways. If the molecules outside the cell are lower than the concentration in the cytosol, the solution is said to be hypotonic to the cytosol, in this process, water diffuses into the cell until equilibrium is established. If the molecules outside the cell are higher than the concentration in the cytosol, the solution is said to be hypertonic to the cytosol, in this process, water diffuses out of the cell until equilibrium exists. If the molecules outside and inside the cell are equal, the solution is said to be isotonic to the cytosol, in this process, water diffuses into and out of the cell at equal rates, causing no net movement of water. In osmosis the cell is selectively permeable, meaning that it only allows certain substances to be transferred into and out of the cell.
potato. To make it a fair test I will make sure that the tests will be
I am going to carry out an experiment to measure the change in mass of
Type of tissue- this will be controlled by using only one type of tissue, which is the potato
At point C (highest sucrose - lowest water concentration), there is no indication that the cell is decreasing further in mass. This is because the potato has become flaccid and no more water can leave the cell. The potato is said to be plasmolysed.
Osmosis in Carrots Background Osmosis is the diffusion of water from a dilute solution to a more concentrated solution through a partially permeable membrane, which allows the pass of water molecules but not solute molecules. [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE]If a cell is placed in a less concentrated solution water enters because the less concentrated solution will have a high concentration of water than the inside of the cell. Once the cell takes in maximum water the cell becomes turgid. If the cell was to be placed in a high concentrated solution, water would leave the cell because the cell would contain a low concentrated solution. So in the low concentrated solution there will be a high concentration of water and in the high concentrated solution there will be a low concentration of water.
-The cells in the body will increase because osmosis causes molecules to move from a less concentrated solution to a more concentrated solution.
When I am not using them I will place them away from my experiment and
The potato tubes in higher concentration sugar solution will shrink
Osmosis is the passage of water molecules from a weaker solution to a stronger solution through a partially permeable membrane. A partially permeable membrane only allows small molecules to pass through, so the larger molecules remain in the solution they originated in. Solute molecule [IMAGE] [IMAGE] Water molecule [IMAGE] The water molecules move into the more concentrated solution. When water enters a plant cell it swells up. The water pushes against the cell wall and the cell eventually contains all that it can hold.