Good Laboratory Practices

GOOD LABORATORY PRACTICES

While performing practical in any laboratory we must follow rules strictly and adopt good laboratory practices. Some of the instructions that everyone should follow are discussed below.

SAFETY IN MICROBIOLOGY LABORATORY

The techniques and the micro-organisms involved in the practical must possess minimum risk to the workers. This is therefore necessary that good practices are adopted always when working with mico-organisms because they can be potentially pathogenic.

All the equipment and culture media must be sterilized properly before performing the experiment.

Preparation of stock culture for inoculum preparation.

Prepared culture media inoculated with prepared inoculum.

The inoculated media incubated and sampled.

Safely disposal of cultures after sterilization.

PROTECTION

 No food item should be brought or consumed inside a microbiology lab.

 Avoid contact of items like pencil, labels, pen etc. with mouth in laboratory.

 Hands must be sterilized immediately after performing practical with a sanitizer/disinfectant.

 Cuts and wounds must be protected properly with bandage.

 All experiments must be performed after proper guidance and under supervision of concerned person.

ASEPTIC TECHNIQUES

Equipment should be sterile while culturing and transferring microbes to avoid any contamination.

Equipment used or contaminated should be autoclaved.

ELECTRICAL SAFETY

Many equipment like bioreactor, weighing scale, stirrer etc. works on electricity now-a-days. No contact of such equipment mains with ant type of liquid should be ensured.

GLASSWARES

Proper handling and sterilization of glassware should be done.

In case of any accidents...

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...not fit for drinking.

REFERENCES

Balasubramanyam MK, BiE, Glotzer. M (2004).“ Comparative analysis of cytokinesis in budding yeast”

“Brewer’s yeast “, University of Maryland, medical centre.

Erhard Friedrich Leuchs (1831) “Wirkung des speichels auf starke.Poggendorff’sahnalen der physik und chemie, volume 22, page 623.

Flickers P, Benelti PH, Wache Y, Marty A, Mauersberger S Smith MS, Nicaud, JM (2005) “ Hydrophobic Substrate utilization by the yeast Yarrowialipolytica, and its potential applications”. FEMS yeast research

History of biology 1800-1849.

Huxley A (1871). “Disourses: Biological and geological ( VolumeIII ) : Yeast”. Collected essays.

Kurtzman, C P. 2006

Legras JL, Merdinogln D, Cornnet J-M, Karst F. (2007). “ Bread, beer and wine: Saccharomyces cerevisiae diversity reflects human history.

Robert hill and Joseph Needham.The chemistry of life.