Find the concentration of sucrose in a potato cell - Plan

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Find the concentration of sucrose in a potato cell - Plan

Plan For Osmosis/Plasmolysis Experiment

AIM

In this experiment, I am trying to find the concentration of sucrose

in a potato cell. Also I can observe when a cell is totally

plasmolyised.

PLAN

I plan to complete this experiment within an hour. Firstly, I will

cut 15 potato chips, 3 each for 5 different solutions, each weighing

1.2grams, cut with a 4mm corer to approx (full word) 4cm. I will measure

weight, as length is less accurate. The weight of the chip may vary as

much as 0.02 g each way, which will make it a lot easier to do, but may

provide me with less accurate results. I will use 5 different molar

solutions of 20 cm3 each, shown to the right. 0M (water), 0.5M, 1M, 1.5M

and 2M. I will create 0.5 molar solutions by mixing 10cm3 of water and

10cm3 of 1 molar solution. I will use 3 of each solution to ensure that

my results are correct, and to prevent any anomalies disturbing my result

graphs. One of each solution will be dropped into the solution at 10-second

intervals. They will be taken out 30 minutes after precisely, to

ensure a reliable result. They will then be dried on a paper towel,

and quickly weighed, and recorded in a pre-written table.

RELIABILITY/ FAIR TEST

I must control many variables in this test. I will control the time

that they are left in the solution, as this will greatly affect

results if they are left in for different amounts of time. Also I must

control how much I dry the chips before weighing them, because if I

leave some excess water on one of them, it will be an unfair test, as

ill be weighing the water as well as the chip. Another point is

simple, but just to make sure that the scales I am weighing the chip

on are at 0 before weighing the chip, as it may add some mass to the

chip. I will use 5 different solutions, starting at 0 molar and going

up in 0.5 molar concentrations. I have chosen this because they are

equidistant, allowing for easy graph drawing, and also because from

the preliminary experiment, I know that this will give me results that

can show me the concentration of sucrose in the cells sap, and also go

on to show me the concentration that the cell will plasmolyise at. I

will also control the variety of potato- I will only use 1 brand, as

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