Introduction
I am picking this topic because I have an interest in enzymes and how they work and how they help us in the world today. The different reactions that you can get from using enzymes and other substances intrigue me. The fact that it reduces prises levels in fruit Juice Company’s is amazing. It is also a great way to see food that have a high H2O2 level in them and find out what foods are bad for the body. I have found it interesting that from what I have been thought in class my come to help me do my ORT.
Topic
This experiment is to see the reactions of the catalyst from different substances or food groups when mixed with H2O2 and different acids. This will be done by using different substances that have high levels of catalyse enzymes. Substances with high levels of catalyst enzymes are Liver, yeast and Cabbage.
Sub Questions
1. What kind of liver will be used?
2. How long will the experiment take?
3. Why does hydrogen peroxide foam when you put it on a catalase?
4. What happens to H2O2 in the processing of the catalase?
5. What acids have the most effect on the Catalyse?
Hypothesis: liver will have the height’s amount of Catalase and the most amount of Foam for all 3 experiments, Catalase + H2O2
Aim: in this experiment is to use this reaction to see what substances have high levels of the Catalase enzymes and if different acids and basses are added would affect the reaction and the enzyme.
Apparatus:
Scale
Blender
Steering Rod
Ruler in cm
6 X 10 ml measuring cylinder
Forceps
Fresh liver
Red cabbage
Yeast
9 X 100 mL measuring cylinder
Spoon
A Black Pen Marker
Sodium Hydroxide
Hydrochloric acid
Hydrogen peroxide
The Method
1. Label the 100 ml measuring cylinder A, B, C, D, E, F, G, H, I wit...
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...e reaction.
Conclusion
The Liver has the highest amount of Catalase and the most amount of Foam for all 3 test tubes and all 3 experiments. With all three of the Catalase the reaction made bubbles the bubbles you see in the foam are pure oxygen bubbles being created by the catalase. From the experiment you can see the Sodium Hydroxide had a big effect on the three different catalysts. It broke down the Yeast and Cabbage so much that hardly any reaction happened, the yeast was broken down so bad that no reaction happened so if you increased the amount or time with the Sodium Hydroxide with the liver it may do the same thing. But with the liver a slow but a big reaction happened. With the bubbles being water (H2O) and oxygen gas (O2). With all my findings it shows that the liver out of all the three catalyst holds the most catalyst and has the biggest reaction.
The purpose of this study is to analyze the activity of the enzyme, catalase, through our understanding
Catalase is a common enzyme that is produced in all living organisms. All living organisms are made up of cells and within the cells, enzymes function to increase the rate of chemical reactions. Enzymes function to create the same reactions using a lower amount of energy. The reactions of catalase play an important role to life, for example, it breaks down hydrogen peroxide into oxygen and water. Our group developed an experiment to test the rate of reaction of catalase in whole carrots and pinto beans with various concentrations of hydrogen peroxide. Almost all enzymes are proteins and proteins are made up of amino acids. The areas within an enzyme speed up the chemical reactions which are known as the active sites, and are also where the
Acting as the controlled group to lessen the effects of all variables except the independent variable, at 0% concentration, the height of foam produced is 0 mm. Attributions to these results is because at 0% substrate concentration, no molecules were present to occupy all the available active sites. As an outcome, the final volume of oxygen is none since there were no collisions taken place between the enzymes and substrate. Therefore, prevented the number of collisions to reach the activation energy.
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
Three flat-bottomed vials were obtained and labeled one through three. 3 mL of hydrogen peroxide was distributed to each of the vials. One drop of liquid soap was then added to each of the vials. The contents of the vials were gently swirled to ensure mixture of the hydrogen peroxide and soap. In each vial, a pH buffer was added; vial one received pH 2 buffer, vial two received pH 7 buffer, and vial three received pH 12 buffer. 1mL of catalase was then placed into each vial and the reaction was timed for 2 minutes. At the end of two minutes, the bubble column produced was measured and recorded into Table 3. The results were then graphed, as shown in Figure
again to try and get as accurate results as possible. Diagram Apparatus Measuring cylinders. Stopwatch.com - a stopwatch. Beaker of water (Heat shield). Beaker.
This enzyme speeds up the break down of hydrogen peroxide into water and oxygen, as enzymes are biological catalysts. [IMAGE]The reaction: Hydrogen peroxide Water + Oxygen Catalase -------- [IMAGE] 2H2O2 2H2O + O2 Apparatus: Hydrogen Peroxide, Several sticks of celery, Stand, boss and clamp, 100ml conical flask, 25cm3 burette, 1800cm3 beaker, Rubber bung with delivery tube, Distilled water, Large container filled with water, 10cm3 measuring cylinder, 10cm3 syringe, 20cm3 syringe, Blender, Knife, Ceramic tile, Electronic balance (correct to 2 decimal places), Sieve, Stopwatch/timer. The variables: There are many possible variables in this investigation, such as pH, temperature, the concentration of substrate and the concentration of the enzyme.
With this information we were able to identify any patterns and similarities. Hypothesis: The higher the temperature of water, potato and H²O², the rate at which the Enzyme will work will be faster therefore producing more oxygen. The reaction will be the same without the catalase (potato). Therefore in both experiments the Enzyme will work more rapidly and produce more oxygen. Aim: To test the hypothesis.
I shall be measuring how much gas is given off. This will be done by measuring the amount of froth on the surface of the liquid. The oxygen released is collected in the form of these bubbles. The equation for the reaction is: (catalase) [IMAGE] H2O2 2H2O + O2 (hydrogen peroxide) (2 part water) (oxygen) I will change the concentration of H2O2 and O2 (making sure the volume stay the same, when one part of a H2O2 particle is taken, an O2 particle is added. Prediction
An error that occurred in the experiment was during the ceric nitrate test because solution 4 should have produced a color change. During a base hydrolysis of aspartame, aspartic acid, phenylalanine and methanol are produced, therefore the ceric nitrate test should have been a positive for alcohol. A reason that this could have shown a negative result is because methanol is a volatile substance and it could have evaporated out, which would have caused a negative ceric nitrate test
In this lab, it was determined how the rate of an enzyme-catalyzed reaction is affected by physical factors such as enzyme concentration, temperature, and substrate concentration affect. The question of what factors influence enzyme activity can be answered by the results of peroxidase activity and its relation to temperature and whether or not hydroxylamine causes a reaction change with enzyme activity. An enzyme is a protein produced by a living organism that serves as a biological catalyst. A catalyst is a substance that speeds up the rate of a chemical reaction and does so by lowering the activation energy of a reaction. With that energy reactants are brought together so that products can be formed.
Researchers then hypothesized that the results would indicate the greatest amount of potato enzyme activity level will take place at room temperature. In this experiment, researchers used potato extract and different temperature levels to test the hypothesis. Moreover, researchers wanted to test the color intensity scale and how specific catechol oxidase is for catechol. In this experiment, researchers used dH2O, catechol solution, hydroquinone, and potato extract. Lastly, researchers tested the substrate concentration and how it has an effect on enzyme activity.
We left these cups sit for twenty- four hours and then we observed them. The second experiment we set up involved dialysis tubing which was acting like a membrane. In the dialysis tubing we put a liquid that was made of starches and sugars. We then put the dialysis tubing into a beaker of water wh... ... middle of paper ... ...