Enzyme Lab Report

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The effects of temperature in enzymes ABSTRACT This report explains enzymes and how their activity can change depending on different factors. We explored this by breaking the experiment into two parts; in the first half we measured peroxidase activity at the different given temperatures and the second half we repeated this but let it sit at room temperature before measuring in the spectrophotometer again. The results showed that temperature does affect the ability of peroxidase. The effects were not reversible. (Enzyme Lab Handout) INTRODUCTION An Enzyme is a biological catalyst, a protein, and is used for many different reactions. Enzymes all have specific shapes that allow them to do different jobs, their shape determines their ability. The …show more content…

Step one of the cycle is the active site, in the active site a particular substrate is permitted to bind to the enzyme, the second step is cofactors or coenzymes that change shape to help it to fit into the active site, the third step is the enzyme-substrate complex when a substrate and an enzyme bind together, and the fourth and final step is where products are released and ready for more reactions. (Enzyme Lab Handout) Our experiment is to find the optimum temperature for Peroxidase, and then see what temperatures the enzyme can be exposed to and if they can recover or not recover normal activity. The purpose of this is to show that enzymes from different organisms may function best at different …show more content…

In order to do this we had to get peroxidase, to obtain it we extracted about 5-7g of potato tissue by blending it with 50 ml of 0.2 M peroxidase; We labeled four 50 ml beakers: peroxidase;; 10 mM H20 2 (substrate); and 25 mM guaiacol (McGraw-Hill Education 2016)which is the dye, so we can see the color change. Then filled each about half full with the appropriate solution. Next we labeled four pipettes to correspond correctly with the beakers. In the first part we determined the effect of temperature on peroxidase activity. To do this activity we measured the temperature after placing the pipettes in 4 degrees Celsius in the ice bath, 22 degrees Celsius, 32 degrees Celsius, and 60 degrees Celsius, at room temperature. Then we checked it every 15 minutes and recorded the data onto one chart. In the second part we repeat the same procedure, but this time we let the solutions return to room temperature which was 60 degrees Celsius after being exposed to each of these temperatures for 15 minutes. We then took them out and let them sit for 15 minutes and measured the activity and recorded the data on the second data chart. The instrument used to measure the activity changes was the spectrophotometer, we recorded the data every twenty seconds. The class all put their data together and found the slope at the different temperatures and at recovery. (McGraw-Hill Education

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