Efficacy of Echinacea on the Action of Cyproterone Acetate in Male Rats

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ABSTRACT

The study aimed to evaluate the effect Echinacea extract (E) on the testicular antioxidants function in normal rats or that subjected to anti-androgenic compound, cyproterone acetate (CA). Rats were divided into 5 groups treated daily via an oral tube for two intervals 2 and 4 weeks, 1st control, 2nd E (63mg/kg), 3rd CA (25mg/kg), 4th E+CA and 5th E as prophylactic one week before E+CA treatment. So CA used as a positive control compound with antiandrogenic activity with proposed testicular oxidant. The body, testes, epididymis and vas deferens weights were recorded. Sperm count, nitric oxide (NO), calcium ion (Ca2+) and malondialdhyde (MDA) contents in addition to superoxide dismutase (SOD), glutathione S-transferase (GST) activities were determined in testicular tissues. CA exhibited direct negative effect on reproductive organs weight and significant reducing effect on sperm count and Ca2+ contents. SOD and GST activities significantly decreased in addition to significant increase in NO, MDA contents reflecting the oxidative status of testis in CA treated rats.

The prophylactic effect of E treatment, in time related manner, showed significant improvement in the antioxidant status of the testicular tissue which is more pronounced as compared to E+CA treatment.

Key wards: Echinacea, cyproterone acetate, reproductive system, antioxidants

INTRODUCTION

The antioxidant system plays an effective role in protecting testes and other biological tissues below a critical threshold of reactive oxygen species (ROS) thus preventing testicular dysfunction (Oschsendorf, 1999). ROS mediated oxidative stress is one of crucial reasons of infertility and decreased sperm viability and increased level of free radicals may cause degener...

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...d local levels such as in the testis, when nuclear receptors are (over) saturated. Androgens can also induce rapid calcium fluxes in a variety of cell types, including human prostatic cancer cells and rat sertoli cells (Steinsapir et al., 1991; Gorczynska and Handelsman, 1995; Gorczyńska-Fjälling, 2004). This suggestion explains the effect of CA treatment on decreasing the testicular Ca ion content.

The study suggests that Echinacea supplementation especially a week before cyproterone acetate shows improvement in the oxidative stress induced by cyproterone acetate treatment. That may be due to the Echinacea antioxidant activity. The anti-androgenic effect of E may in addition to the anti-inflammatory and immune enhancing activity add valuable for using it as a co-treatment for male contraceptives or in treatment of prostate cancer which needs more investigations.

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