Garbage enzymes were produced based on the formulation of three parts of fruit peels, one part of brown sugar and ten parts of water were mixed together (The Star, 2009) and fermented in 5L plastic bottle at room temperature for three months.
CHARACTERISTICS OF GARBAGE ENZYME.
The progression of fermentation of the fruit discards showed a reduction of pH with times, 3.15 for GE while 3.78 for CGE. Odunfa, (1985) attributed the significant reduction of pH to acid production during fermentation. In our study, the fall of pH could be attributed to the organic acids produced during fermentation by microorganisms. The pH value of GE produced was different with CGE. This might be due to the fruit types used in the production and fermentation practice was different. For instance, the fruit peels collected were cleaned with distilled water while the commercial producer would ferment them right away without washing. Moreover, natural fermentation is spontaneous and uncontrolled thus resulting in a product of variable quality.
Total microbial counts were detected to be about 5 log CFU/ml by the end of fermentation for both GE and CGE. On the other hand, total yeast and mold count was really low for both GE and CGE. From the observation morphology of the colony on the DRBC agar, only yeasts were present in our fermentation products.
Fruit peels collected were cleaned using only distilled water, and were not subjected to further pre-treatment before fermentation. Spontaneous fermentations are generally uncontrolled and the product microbiota is inconsistent. The fermentation mainly relies on the microflora from the fruit surfaces and to some extent from the utensils used during the fermentation process. The diverse microbial flora on fruit ...
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...ewater. World Academy of Science, Engineering and Technology. 60: 1143-1148.
The Star. 2009. http://www.thestar.com.my/story.aspx?file=%2f2008%2f7%2f26%2flifefocus%2f1633121&sec=lifefocus
Village, P.K.S. 1998. Characterization of Municipal Solid Waste in the United Satte. 1997 update. US Environmental Protection Agency Municipal and Industrial Solid Waste Division.
Wang, J.Y., Xu, H.L. & Tay, J.H., 2002. A hybrid two-phase systems for anaerobic digestion of food waste. Water Science and Technology. 45: 159-165.
Wang, Q.H., Ma, H.Z., Xu, W.L., Gong, L.J., Zhang, W.Y. & Zou, D.X. 2008. Ethanol production from kitchen garbage using response surface methodology. Chemical Engineering Journal. 39: 604-610.
Wang, X.Q., Wang, Q.H., Liu, Y.Y. & Ma, H.Z. 2009. On-site production of crude glucoamylase for kitchen waste hydrolysis. Waste Management & Research. 28: 539-544.
After 48 hours of incubation the agar plates were viewed. Individual colonies were tested for successful isolation by gram staining and then viewing the stained bacteria under a microscope. Isolation was successful. One colony of each unknown bacteria was transferred to an agar slant for growth. The agar slants were stored at room temperature over the weekend so that they would not grow too much.
The hypothesis that states that “if pectinase and cellulase are added into different containers of applesauce, then pectinase will produce the most apple juice because pectin, which is very abundant in applesauce, is broken down by pectinase to make apple juice” is supported by the data. According to the data, when water was added to applesauce, the amount of apple juice created on average was 8.2 ml. When cellulase was added to applesauce, the amount of apple juice created on average was 8.26 ml. Both of these substances created less apple juice on average than when pectinase was added to applesauce. The data shows that on average the amount of apple juice created with the assistance of pectinase was 13.16 ml. Therefore, the conclusion can be made that the enzyme, pectinase, extracts the most apple juice out of apple sauce. This conclusion can also be supported by the data found by members of the class. The average amount of apple juice
The purpose of this project was to identify unknown bacteria species from a mixed culture. The two unknown species were initially plated onto Tryptic Soy Agar (TSA), Eosin Methylene Blue (EMB), Mannitol Salt Agar (MSA), and blood agar plates to distinguish between the two different bacteria using colony size, color, shape, and growth characteristics. By identifying and inoculating the differing types of colonies, the two unknown bacteria were purified and able to be tested
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
Fermentation is a form of chemical transformation of organic substances that breaks down simple compounds by exploiting the enzymes with compl...
Despite of general properties of enzymes, the properties also varies from where it comes from and how it been produced. For instance, the enzymatic saccharification method in lignocellulosic bioethanol is generated by hydrolyzing cellulose and hemicelluloses. This method gets high attention because of its higher theoretical yield compared to other methods (Taneda et al., 2012). Acremonium cellulolyticus with high activities of cellulase, amylase and pectinase enzymes allow it for the easy separation of solids/liquids in potato pulp, resulting in high saccharification efficiency and a high recovery rate of products (Gao et al., 2014). On the other hand, Enzyme-modified carboxymethyl starch (ECMS) is beneficial in enhancing water holding capacity, emulsion stability and improving sensory characteristics of sausages with a declined fat content (Luo and Xu, 2011). Lipases and phospholipases of dormant cotton seeds have stability in heat, various media and nature of the hydrolysis of the lipids properties (Rakhi...
By taking a Carbon Dioxide, rich substance and mixing it with a yeast, solution fermentation will occur, and then it could be determined if it is a good energy-producer. In this study glacatose, sucrose, glycine, glucose, and water were used to indicate how fast fermentation occurred. The overall result shows that monosaccharides in particular galactose and glucose were the best energy source for a cell.
The freshly squeezed citrus juices of Lemon,lime and orange were analysed through titration over the period of 2 weeks. Initially the lime juice was found to be slightly more acidic than the other juices followed by lemon juice and then orange juice since it is known that usually lime is more acidic than lemon and orange. It was hypothesised that the acidity of each juice will increase or decrease after the period of two weeks depending upon the sugar content of that citrus fruit that the juice is extracted from. The acidity of the juice with the highest sugar level will gradually increase over the period of time since sugar contribute to the the fermentation of the citrus juice to lactic acid. The hypothesis was shown to be correct;however
Yang, S.-S., Guo, W.-Q., Zhou, X.-J., Meng, Z.-H., Liu, B., Ren, N.-Q., 2011. Optimization of operating parameters for sludge process reduction under alternating aerobic/oxygen-limited conditions by response surface methodology. Bioresour. Technol. 102, 9843–9851.
Law, Abu Bakar, Mat Hashim, and Abdul Hamid (2011) concluded that fermentation is one of the oldest and widely used food preservation methods in households, small-scale food industries as well as in large enterprise. Fermented foods generally preserved pleasant flavor, aroma, texture, enhanced nutritive values and good keeping quality under ambient conditions. (p.1)
We were required to conduct an experiment that explores and analyses the fermentation of the fruits of the Marula tree. We produced two bottles (750ml each) of high quality Marula wine. Our first bottle of wine (Valley Wine) was our control and our second bottle of wine (Maritchi) contained our changed variable.After reading up on various wine recipes, we noticed that many suggested the use of pectinase in the fermentation process. We thus decided to use the addition of litchi pulp and juice as our changed variable in our second bottle of wine because we wanted to test if it would act like a natural pectic enzyme halfway through the fermentation process of the Marula wine. Both wines were assessed by a panel of judges on colour, clarity, bouquet, taste and presentation.
Culture plates of yeasts strains: S41, a pet 1 and M240, conical flasks containing Yeast Extract Potassium Acetate (YEPA), Yeast Extract Peptone Dextrose (YEPD) and Yeast Extract Palm Olein (YEPPO) media, pH indicator, inoculation loop, microscope, methylene blue, Bunsen burner and incubator.
The process of alcoholic fermentation begins with the use of enzymes. The enzymes begin to break down the long chains in starch molecules, a polysaccharide that consists of a large quantity of glucose molecules (C6H12O6) joined by glycosidic bonds as seen in figure 1, into single glucose molecules, a monosaccharide with six carbons and five hydroxyl groups. After the starch has become sugar, the enzymes are used once again, this time to convert the sugars into ethyl alcohol and carbon dioxide, CO2, as seen in figure 2 (World of Scientific Discovery, 2007). The carbon dioxide produced is released into the atmosphere, leaving water and ethanol, the alcohol, behind. Ethanol is a colorless flammable liquid with a molecular formula of C2H6O, giving it a molar mass of 46.07 grams per mole. Ethanol is also characterized by a melting point of -114°C or 159 K.
Nowadays the soft drinks industry is booming, alcohols are not as successful as before and sodas are pointed out for their high sugar content. This is profitable for bio fruit beverages and other fruit based alternative beverages. This market is full of innovative ideas and opportunities.
NREL (National Renewable Energy Laboratory), USA reported that in 2010 there was approximately 5 billion gallons/ annum ethanol was produced using food grains and production of 8 billion gallons/ annum ethanol is expected in 2015 with the use of lignocellulosic biomass. Major constituents of biomass containing polymers of sugars are cellulose and hemicelluloses whereas lignin forms a protective covering against various in...