Drosophila Experiment Lab Report

Phenotypic Patterns of Drosophila

Introduction
The purpose of this experiment is to show and apply the principles of Mendelian laws by experimenting with Drosophila melanogaster fruit flies. There were different assignments done to observe the evidences of certain traits, including sex-linked traits, inherited to the next generation and so on. All series of fruit fly test are analyzed to find the concluded results. The final results will show the phenotypic ratio of the fruit flies and the expected frequencies of distribution are calculated to see if the expected results are true.
Hypothesis
Monohybrid cross between two parents with specifically chosen traits should result in a 3:1 ratio. Dihybrid cross should demonstrate a 9:3:3:1 ratio after

The female parent has a black body and the male is a wild type. Both flies are unpacked and then transferred into a mating jar. The parent flies mated and laid eggs. In two weeks, the eggs are hatched into larvae, molted into pupae and developed into adults. The flies are then knocked out by using ether, an anesthetic chemical, and placed on a petri dish for examination. Traits of both male and female flies were separated, observed and identified. The process is the same for the other experiments except for the dihybrid cross test where the F1 generation mates to create the F2 generation for further observation. The uncontrolled portion of the experiments were the flies with specific traits chosen for each

A wild-type male and a white eyes female mated to produce the F1 generation. The F1 generation consisted of 602 females with wild-type and 580 males with white eyes. A total of 1182 have been observed and a 1:1 ratio was expected.
Generation Sex Trait Amount Chi-Square
F1 Female Wild-type 602 0.2047
F1 Male White Eyes 580 0.2047

Assignment Bα: The experiment was switched with the female having the wild-type trait and male having the white eyes. The F1 generation of this cross consisted of 582 females with wild-type and 582 males with wild-type. A total of 1164 flies were observed and a 1:1 ratio was expected.
Generation Sex Trait Amount Chi-Square
F1 Female Wild-type 582 0.0000
F1 Male Wild-type 582 0.0000

Assignment C: Assignment C required one fly with two traits on two different chromosomes. A wild-type female and a purple eyes and ebony body male was chosen. The F1 generation consisted of 592 females with wild-type and 614 males with wild-type. A total of 1206 flies were observed and a 1:1 ratio was